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Podocalyxin-Like (PODXL) antibody

Antigen

Podocalyxin-Like (PODXL)

Synonyms PC, PCLP, Gp200, MGC138240, Ly102, Pclp1, PCLP-1, AW121214, podocalyxin, PODXL, podxl, fc18d02, wu:fc18d02
Clonality Monoclonal (TRA-1-60)
Host
Alternatives

Mouse

Reactivity
Alternatives

Human

Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
2 references available
Catalog no. ABIN152150
Quantity 0.1 ml  (4.1 mg/ml)  (Variants)
Price Product not available in this region.
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Additional Information

Alternative name TRA-1-60
Gene ID 5420
Immunogen Human embryonal carcinoma cell line 2102Ep.
Format Ascites
Isotype IgM
Clone TRA-1-60
Description TRA-1-60 is a cell-surface protein involved in cell differentiation. Cell surface antigensare powerful tools for characterizing the function and expression of developmentallyregulated cells during both embryogenesis and oncogenesis. Alternate Names: anti-TRA160 antibody.
Specificity TRA-1-60 recognizes antigens that are associated with a pericellular matrixproteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope. Species Reactivity: This antibody reacts with the neuraminidase resistant form of the TRA-1-60 antigen thatis expressed upon the surface of human teratocarcinoma stem cells (EC), humanembryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivityis seen with murine EC, EG or ES cells. Localization: Cell Membrane.

Application Details

Application Notes This antibody may be used in FACS analysis, immunohistochemistry,immunofluorescence, western blot and immunoprecipitation (1,2).
Concentration 4.1 mg/ml
Buffer Preservative: 0.1% sodium azide.
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Restrictions For Research Use only

Publications

Badcock, Pigott, Goepel et al.: "The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan." in: Cancer research, Vol. 59, Issue 18, pp. 4715-9, 1999 (PubMed).

Matin, Walsh, Gokhale et al.: "Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells." in: Stem cells (Dayton, Ohio), Vol. 22, Issue 5, pp. 659-68, 2004 (PubMed).

Alternatives

Alternatives for antigen "Podocalyxin-Like (PODXL)", type "Antibodies"
Hosts Mouse (15), Rabbit (11), Goat (4), Rat (4), Chicken (2)
Reactivities Human (28), Mouse (Murine) (7), Rat (Rattus) (2), Dog (Canine) (1)
Applications Western Blotting (WB) (26), ELISA (12), Flow Cytometry (FACS) (11), Immunohistochemistry (IHC) (6), Immunocytochemistry (ICC) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunofluorescence (IF) (2), Immunoprecipitation (IP) (2), Dot Blot (DB) (1)
Conjugates Biotin (1), FITC (1), PE (1)
Epitopes AA 36-236 (2), C-Term (2), Extracellular Domain (2), Internal Region (1), N-Term (1)