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HIF1A / HIF1 alpha Sampler (AA 329-530), (AA 432-528) antibody

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AA 432-528, AA 329-530
Human, Sheep (Ovine), Mouse (Murine), Rat (Rattus), Rabbit, Ferret, Monkey, Cow (Bovine)
Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
Pubmed 12 references available
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Quantity 5 vials
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Immunogen NB100-105, NB100-134 = Fusion protein containing amino acids 432-528 of human HIF-1 alpha. NB100-296 = Fusion protein containing amino acids 329-530 of human HIF-1 alpha. NB100-449 = A synthetic peptide mapping to a region between residues 775 and the C
Kit Components NB100-105: HIF-1 alpha Antibody (H1alpha67), NB100-449: HIF-1 alpha Antibody, NB100-479: HIF-1 alpha Antibody, NB100-134: HIF-1 alpha Antibody, NB100-296: HIF-1 alpha Antibody (HA111)
Isotype IgG
Specificity These antibodies are specific for HIF-1 alpha.
Background Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein that activates gene transcription in response to reducedcellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, hemeoxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is aheterodimer composed of HIF-1a and HIF-1b subunits. Both subunits are induced byhypoxia and rapidly decay upon return to normoxia and are basic-helix-loop-helix-PASproteins. Recent research indicates the ability to regulate hypoxia-inducible factors maybe related to tumor-related angiogenesis in certain cancers.
Gene Symbol: HIF1A
Gene ID 3091
Application Notes By Western blot, these antibodies recognize a band ~120 kDa, representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 120 kDa representing post-translational modification of HIF-1 alpha. Nuclear extracts should be used for Western analysis.
Recommended dilutions: Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot 1:500 -1:1000
Restrictions For Research Use only
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-HIF1A / HIF1 alpha Sampler (AA 329-530), (AA 432-528) antibody (ABIN152187) Western Blot analysis of ABIN151066, anti-HIF-1 alpha, on normoxic and hypoxic nuclea...
Western Blotting (WB) image for anti-HIF1A / HIF1 alpha Sampler (AA 329-530), (AA 432-528) antibody (ABIN152187) anti-HIF1A / HIF1 alpha Sampler (AA 329-530), (AA 432-528) antibody (Image 2)
Western Blotting (WB) image for anti-HIF1A / HIF1 alpha Sampler (AA 329-530), (AA 432-528) antibody (ABIN152187) Detection of mouse HIF1-alpha unsing ABIN151900
Product cited in: Wang, Kiss, Franklin et al.: "Different cellular traffic of LDL-cholesterol and acetylated LDL-cholesterol leads to distinct reverse cholesterol transport pathways." in: Journal of lipid research, Vol. 48, Issue 3, pp. 633-45, 2007 (PubMed).

Grimmer, Balbus, Lang et al.: "Regulation of type II collagen synthesis during osteoarthritis by prolyl-4-hydroxylases: possible influence of low oxygen levels." in: The American journal of pathology, Vol. 169, Issue 2, pp. 491-502, 2006 (PubMed).

Villanueva, Cespedes, Gonzalez et al.: "bFGF induces an earlier expression of nephrogenic proteins after ischemic acute renal failure." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 291, Issue 6, pp. R1677-87, 2006 (PubMed).

Chade, Zhu, Mushin et al.: "Simvastatin promotes angiogenesis and prevents microvascular remodeling in chronic renal ischemia." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 10, pp. 1706-8, 2006 (PubMed).

Galabova-Kovacs, Matzen, Piazzolla et al.: "Essential role of B-Raf in ERK activation during extraembryonic development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 5, pp. 1325-30, 2006 (PubMed).

Boisvert, Rose, Boullier et al.: "Leukocyte transglutaminase 2 expression limits atherosclerotic lesion size." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 26, Issue 3, pp. 563-9, 2006 (PubMed).

Karlsson, Edsjoe, Puahlman et al.: "Multidrug-resistant neuroblastoma cells are responsive to arsenic trioxide at both normoxia and hypoxia." in: Molecular cancer therapeutics, Vol. 4, Issue 7, pp. 1128-35, 2005 (PubMed).

Forcheron, Legedz, Chinetti et al.: "Genes of cholesterol metabolism in human atheroma: overexpression of perilipin and genes promoting cholesterol storage and repression of ABCA1 expression." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 25, Issue 8, pp. 1711-7, 2005 (PubMed).

Covello, Simon, Keith: "Targeted replacement of hypoxia-inducible factor-1alpha by a hypoxia-inducible factor-2alpha knock-in allele promotes tumor growth." in: Cancer research, Vol. 65, Issue 6, pp. 2277-86, 2005 (PubMed).

Kong, Eltzschig, Karhausen et al.: "Leukocyte adhesion during hypoxia is mediated by HIF-1-dependent induction of beta2 integrin gene expression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 28, pp. 10440-5, 2004 (PubMed).

Joegi, uora, Nilsson et al.: "Hypoxia alters gene expression in human neuroblastoma cells toward an immature and neural crest-like phenotype." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 10, pp. 7021-6, 2002 (PubMed).

Background publications Haase, Glickman, Socolovsky et al.: "Vascular tumors in livers with targeted inactivation of the von Hippel-Lindau tumor suppressor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 4, pp. 1583-8, 2001 (PubMed).

Catalog No. ABIN152187
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