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HIF1A / HIF1 alpha Sampler (AA 329-530), (AA 432-528) antibody

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AA 432-528, AA 329-530
Human, Sheep (Ovine), Mouse (Murine), Rat (Rattus), Rabbit, Ferret, Monkey, Cow (Bovine)
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen NB100-105, NB100-134 = Fusion protein containing amino acids 432-528 of human HIF-1 alpha. NB100-296 = Fusion protein containing amino acids 329-530 of human HIF-1 alpha. NB100-449 = A synthetic peptide mapping to a region between residues 775 and the C
Kit Components NB100-105: HIF-1 alpha Antibody (H1alpha67), NB100-449: HIF-1 alpha Antibody, NB100-479: HIF-1 alpha Antibody, NB100-134: HIF-1 alpha Antibody, NB100-296: HIF-1 alpha Antibody (HA111)
Isotype IgG
Specificity These antibodies are specific for HIF-1 alpha.
Background Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein that activates gene transcription in response to reducedcellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, hemeoxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is aheterodimer composed of HIF-1a and HIF-1b subunits. Both subunits are induced byhypoxia and rapidly decay upon return to normoxia and are basic-helix-loop-helix-PASproteins. Recent research indicates the ability to regulate hypoxia-inducible factors maybe related to tumor-related angiogenesis in certain cancers.
Gene Symbol: HIF1A
Gene ID 3091
Application Notes By Western blot, these antibodies recognize a band ~120 kDa, representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 120 kDa representing post-translational modification of HIF-1 alpha. Nuclear extracts should be used for Western analysis.
Recommended dilutions: Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot 1:500 -1:1000
Restrictions For Research Use only
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-HIF1A / HIF1 alpha Sampler (AA 329-530), (AA 432-528) antibody (ABIN152187) Western Blot analysis of ABIN151066, anti-HIF-1 alpha, on normoxic and hypoxic nuclea...
Western Blotting (WB) image for anti-HIF1A / HIF1 alpha Sampler (AA 329-530), (AA 432-528) antibody (ABIN152187) anti-HIF1A / HIF1 alpha Sampler (AA 329-530), (AA 432-528) antibody (Image 2)
Western Blotting (WB) image for anti-HIF1A / HIF1 alpha Sampler (AA 329-530), (AA 432-528) antibody (ABIN152187) Detection of mouse HIF1-alpha unsing ABIN151900
Product cited in: Wang, Kiss, Franklin, McBride, Whitman, Marcel: "Different cellular traffic of LDL-cholesterol and acetylated LDL-cholesterol leads to distinct reverse cholesterol transport pathways." in: Journal of lipid research, Vol. 48, Issue 3, pp. 633-45, 2007

Galabova-Kovacs, Matzen, Piazzolla, Meissl, Plyushch, Chen, Silva, Baccarini: "Essential role of B-Raf in ERK activation during extraembryonic development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 5, pp. 1325-30, 2006

Grimmer, Balbus, Lang, Aigner, Cramer, Müller, Swoboda, Pfander: "Regulation of type II collagen synthesis during osteoarthritis by prolyl-4-hydroxylases: possible influence of low oxygen levels." in: The American journal of pathology, Vol. 169, Issue 2, pp. 491-502, 2006

Chade, Zhu, Mushin, Napoli, Lerman, Lerman: "Simvastatin promotes angiogenesis and prevents microvascular remodeling in chronic renal ischemia." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 10, pp. 1706-8, 2006

Boisvert, Rose, Boullier, Quehenberger, Sydlaske, Johnson, Curtiss, Terkeltaub: "Leukocyte transglutaminase 2 expression limits atherosclerotic lesion size." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 26, Issue 3, pp. 563-9, 2006

Villanueva, Cespedes, Gonzalez, Vio: "bFGF induces an earlier expression of nephrogenic proteins after ischemic acute renal failure." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 291, Issue 6, pp. R1677-87, 2006

Covello, Simon, Keith: "Targeted replacement of hypoxia-inducible factor-1alpha by a hypoxia-inducible factor-2alpha knock-in allele promotes tumor growth." in: Cancer research, Vol. 65, Issue 6, pp. 2277-86, 2005

Forcheron, Legedz, Chinetti, Feugier, Letexier, Bricca, Beylot: "Genes of cholesterol metabolism in human atheroma: overexpression of perilipin and genes promoting cholesterol storage and repression of ABCA1 expression." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 25, Issue 8, pp. 1711-7, 2005

Karlsson, Edsjoe, Puahlman, Pettersson: "Multidrug-resistant neuroblastoma cells are responsive to arsenic trioxide at both normoxia and hypoxia." in: Molecular cancer therapeutics, Vol. 4, Issue 7, pp. 1128-35, 2005

Kong, Eltzschig, Karhausen, Colgan, Shelley: "Leukocyte adhesion during hypoxia is mediated by HIF-1-dependent induction of beta2 integrin gene expression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 28, pp. 10440-5, 2004

Joegi, uora, Nilsson, Lindeheim, Makino, Poellinger, Axelson, Puahlman: "Hypoxia alters gene expression in human neuroblastoma cells toward an immature and neural crest-like phenotype." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 10, pp. 7021-6, 2002

Background publications Haase, Glickman, Socolovsky, Jaenisch: "Vascular tumors in livers with targeted inactivation of the von Hippel-Lindau tumor suppressor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 4, pp. 1583-8, 2001