Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Details for Product No. ABIN152188
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

 
Antigen
Synonyms HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hif1a, HIF1-alpha, HIF-1a, HIF1a, hif-1a
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(245), (101), (80), (56), (38), (34), (20), (19), (17), (16), (12), (2), (2), (2), (1), (1)
Host
Mouse
(174), (112), (2), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(27), (14), (12), (12), (12), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(231), (79), (79), (67), (58), (50), (36), (28), (15), (11), (10), (9), (7), (4), (1), (1)
Pubmed 4 references available
Catalog no. ABIN152188
Quantity 4 vials
Price
Contact our Customer Service for availability and price in your country.
Shipping to United States (Change)
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen NB 100-131: Human HIF-1 alpha amino acids 329-530.NB100-123: Fusion protein containing amino acids 432-528 of human HIF-1alpha.NB100-449: peptide between residues 775 and the C-terminus (residue 826) of humanHIF-1 alpha.ABIN151926: A fusion protein including residues 530-825 of the mouse HIF-1 alphaprotein.
Isotype IgG
Specificity These antibodies are specific for HIF-1alpha.
Alternative Name HIF1A / HIF1 alpha sampler
Background Our HIF-1 alpha sample pack provides a convenient way to determine the optimal HIF-1alpha antibody for your specific species and tissues. Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein that activates gene transcription in response to reducedcellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, hemeoxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is aheterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits areinduced by hypoxia and rapidly decay upon return to normoxia and arebasic-helix-loop-helix-PAS proteins. Recent research indicates the ability to regulatehypoxia-inducible factors may be related to tumor-related angiogenesis in certaincancers.
Gene ID 3091
Application Notes Immunohistochemical staining of paraffin-embedded and frozen sections (only NB100-131 has been used on frozen sections). Antigen retrieval is strongly recommended.With the exception of NB 100-449 and ABIN151926 (which are also recommended forWB), these antibodies work for Western blot analysis but are not specificallyrecommended for this application. Suggested working dilutions: *Immunohistochemistry - 1:100 -1:5,000 ****A starting dilution of 1:1,000 is recommended with titration up or down dependingupon the signal and background.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Supplier Images
anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody HIF-1 Alpha staining in hypoxia-induced human placenta using ABIN151061.
anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody (2) Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section.
anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody (3) Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using ABIN151061. Blue dye is DAPI nuclear staining. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
General Tanaka, Kojima, Ohse et al.: "Hypoxia-inducible factor modulates tubular cell survival in cisplatin nephrotoxicity." in: American journal of physiology. Renal physiology, Vol. 289, Issue 5, pp. F1123-33, 2005 (PubMed).

Quintero, Brennan, Thomas et al.: "Nitric oxide is a factor in the stabilization of hypoxia-inducible factor-1alpha in cancer: role of free radical formation." in: Cancer research, Vol. 66, Issue 2, pp. 770-4, 2006 (PubMed).

Higami, Barger, Page et al.: "Energy restriction lowers the expression of genes linked to inflammation, the cytoskeleton, the extracellular matrix, and angiogenesis in mouse adipose tissue." in: The Journal of nutrition, Vol. 136, Issue 2, pp. 343-52, 2006 (PubMed).

Hill, Shukla, Tran et al.: "Inhibition of hypoxia inducible factor hydroxylases protects against renal ischemia-reperfusion injury." in: Journal of the American Society of Nephrology : JASN, Vol. 19, Issue 1, pp. 39-46, 2008 (PubMed).

Hosts (174), (112), (2), (1)
Reactivities (245), (101), (80), (56), (38), (34), (20), (19), (17), (16), (12), (2), (2), (2), (1), (1)
Applications (231), (79), (79), (67), (58), (50), (36), (28), (15), (11), (10), (9), (7), (4), (1), (1)
Conjugates (27), (14), (12), (12), (12), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (36), (23), (15), (10), (8), (7), (7), (6), (6), (6), (5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
back to top