Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Antigen: Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A)

Synonyms: HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A, HIF-1a, HIF1a, hif-1a, hif1a

Clonality: Monoclonal (2 clones)

Host: Mouse

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN152188

Additional Information

Alternative name: HIF1A / HIF1 alpha sampler

Gene ID: 3091

Immunogen: NB 100-131: Human HIF-1 alpha amino acids 329-530.NB100-123: Fusion protein containing amino acids 432-528 of human HIF-1alpha.NB100-449: peptide between residues 775 and the C-terminus (residue 826) of humanHIF-1 alpha.ABIN151926: A fusion protein including residues 530-825 of the mouse HIF-1 alphaprotein.

Isotype: IgG

Clone: 2 clones

Description: Our HIF-1 alpha sample pack provides a convenient way to determine the optimal HIF-1alpha antibody for your specific species and tissues. Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein that activates gene transcription in response to reducedcellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, hemeoxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is aheterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits areinduced by hypoxia and rapidly decay upon return to normoxia and arebasic-helix-loop-helix-PAS proteins. Recent research indicates the ability to regulatehypoxia-inducible factors may be related to tumor-related angiogenesis in certaincancers.

Specificity: These antibodies are specific for HIF-1alpha.

Application Details

Application Notes: Immunohistochemical staining of paraffin-embedded and frozen sections (only NB100-131 has been used on frozen sections). Antigen retrieval is strongly recommended.With the exception of NB 100-449 and ABIN151926 (which are also recommended forWB), these antibodies work for Western blot analysis but are not specificallyrecommended for this application. Suggested working dilutions: *Immunohistochemistry - 1:100 -1:5,000 ****A starting dilution of 1:1,000 is recommended with titration up or down dependingupon the signal and background.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

HIF-1 Alpha staining in hypoxia-induced human placenta using ABIN151061.

Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section.

Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using ABIN151061. Blue dye is DAPI nuclear staining. Photos courtesy of Susan Alexander and Hattie Gresham, PhD.

Publications

Tanaka, Kojima, Ohse et al.: "Hypoxia-inducible factor modulates tubular cell survival in cisplatin nephrotoxicity." in: American journal of physiology. Renal physiology, Vol. 289, Issue 5, pp. F1123-33, 2005 (PubMed).

Quintero, Brennan, Thomas et al.: "Nitric oxide is a factor in the stabilization of hypoxia-inducible factor-1alpha in cancer: role of free radical formation." in: Cancer research, Vol. 66, Issue 2, pp. 770-4, 2006 (PubMed).

Higami, Barger, Page et al.: "Energy restriction lowers the expression of genes linked to inflammation, the cytoskeleton, the extracellular matrix, and angiogenesis in mouse adipose tissue." in: The Journal of nutrition, Vol. 136, Issue 2, pp. 343-52, 2006 (PubMed).

Hill, Shukla, Tran et al.: "Inhibition of hypoxia inducible factor hydroxylases protects against renal ischemia-reperfusion injury." in: Journal of the American Society of Nephrology : JASN, Vol. 19, Issue 1, pp. 39-46, 2008 (PubMed).