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HIF2A / HIF2 alpha (Sample Pack) antibody
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA
|11 references available|
|Price||Product not available in this region.|
NB 100-122 = Proprietary peptide derived from the mouse/human HIF-2a
NB 100-132 = Human HIF-2a amino acids 535-631
ABIN151927 = A fusion protein from mouse HIF-2 alpha, residues 355-438.
|Cross-Reactivity||Human, Mouse (Murine), Rat (Rattus)|
|Description||Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humansand endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functionsprimarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2gene, which is known to be selectively expressed in endothelial cells.|
|Specificity||These antibodies are specific for HIF-2a. Species Reactivity: NB 100-122 recognizes human, mouse, and rat HIF-2a. Other species have not beentested.NB 100-132 reacts with human and rat HIF-2 alpha. Results in mouse havebeen mixed (some + and some -). Other species have not been tested.ABIN151927reacts with both human and mouse proteins. Other species have not been tested.|
|Application Notes||By Western blot, these antibodies recognize a band ~118 kDa, representing HIF-2a ininduced tissues and cells. The monoclonal has been used for immunohistochemistry. The polyclonal has also been used, but success has varied.Suggested working dilutions: *Western Blot - 1:500 -1:1,000+ **Immunohistochemistry - Varies (try 1:100~1,000)**Nuclear extracts should be used for Western analysis, if possible|
|Buffer||1 vial each of NB 200-340 Rabbit anti-CARM1NB 200-341 Rabbit anti-CARM1 and NB 200-342 Rabbit anti-CARM1 Tris-citrate/phosphate pH 7-8 & 0.1% Sodium Azide|
|Storage||Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.|
|Restrictions||For Research Use only|
|Western Blot analysis on normoxic and hypoxic nuclear rat cell lysates using ABIN151051, anti-HIF-2 alpha anti-HIF2A / HIF2 alpha (Sample Pack) antibody (Image 2) anti-HIF2A / HIF2 alpha (Sample Pack) antibody (Image 3)|
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