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Details for Product No. ABIN152189

HIF2A / HIF2 alpha (Sample Pack) antibody

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Antigen
Reactivity
Human
Host
Clonality Polyclonal
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA
Pubmed 11 references available
Catalog no. ABIN152189
Quantity 2 vials
Price
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Immunogen NB 100-122 = Proprietary peptide derived from the mouse/human HIF-2a
NB 100-132 = Human HIF-2a amino acids 535-631
ABIN151927 = A fusion protein from mouse HIF-2 alpha, residues 355-438.
Specificity These antibodies are specific for HIF-2a. Species Reactivity: NB 100-122 recognizes human, mouse, and rat HIF-2a. Other species have not beentested.NB 100-132 reacts with human and rat HIF-2 alpha. Results in mouse havebeen mixed (some + and some -). Other species have not been tested.ABIN151927reacts with both human and mouse proteins. Other species have not been tested.
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Background Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humansand endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functionsprimarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2gene, which is known to be selectively expressed in endothelial cells.
Gene ID 2034
Application Notes By Western blot, these antibodies recognize a band ~118 kDa, representing HIF-2a ininduced tissues and cells. The monoclonal has been used for immunohistochemistry. The polyclonal has also been used, but success has varied.Suggested working dilutions: *Western Blot - 1:500 -1:1,000+ **Immunohistochemistry - Varies (try 1:100~1,000)**Nuclear extracts should be used for Western analysis, if possible
Restrictions For Research Use only
Buffer 1 vial each of NB 200-340 Rabbit anti-CARM1NB 200-341 Rabbit anti-CARM1 and NB 200-342 Rabbit anti-CARM1 Tris-citrate/phosphate pH 7-8 & 0.1% Sodium Azide
Preservative Sodium azide
Storage 4 °C
Kuznetsova, Meller, Schnell et al.: "von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif and targets it for ubiquitination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2706-11, 2003 (PubMed).

Mascio, Olison, Ralphe et al.: "Myocardial vascular and metabolic adaptations in chronically anemic fetal sheep." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 289, Issue 6, pp. R1736-45, 2005 (PubMed).

Calvani, Rapisarda, Uranchimeg et al.: "Hypoxic induction of an HIF-1alpha-dependent bFGF autocrine loop drives angiogenesis in human endothelial cells." in: Blood, Vol. 107, Issue 7, pp. 2705-12, 2006 (PubMed).

Schultz, Fanburg, Beasley: "Hypoxia and hypoxia-inducible factor-1alpha promote growth factor-induced proliferation of human vascular smooth muscle cells." in: American journal of physiology. Heart and circulatory physiology, Vol. 290, Issue 6, pp. H2528-34, 2006 (PubMed).

Hui, Bauer, Striet et al.: "Calcium signaling stimulates translation of HIF-alpha during hypoxia." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 466-75, 2006 (PubMed).

Mizukami, Fujiki, Duerr et al.: "Hypoxic regulation of vascular endothelial growth factor through the induction of phosphatidylinositol 3-kinase/Rho/ROCK and c-Myc." in: The Journal of biological chemistry, Vol. 281, Issue 20, pp. 13957-63, 2006 (PubMed).

Nakamura, Martin, Jackson et al.: "Brain-derived neurotrophic factor activation of TrkB induces vascular endothelial growth factor expression via hypoxia-inducible factor-1alpha in neuroblastoma cells." in: Cancer research, Vol. 66, Issue 8, pp. 4249-55, 2006 (PubMed).

Shinojima, Oya, Takayanagi et al.: "Renal cancer cells lacking hypoxia inducible factor (HIF)-1alpha expression maintain vascular endothelial growth factor expression through HIF-2alpha." in: Carcinogenesis, Vol. 28, Issue 3, pp. 529-36, 2007 (PubMed).

Khacho, Mekhail, Pilon-Larose et al.: "Cancer-causing mutations in a novel transcription-dependent nuclear export motif of VHL abrogate oxygen-dependent degradation of hypoxia-inducible factor." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 302-14, 2007 (PubMed).

Inoue, Yanagi, Matsuura et al.: "Expression of hypoxia-inducible factor 1alpha and 2alpha in choroidal neovascular membranes associated with age-related macular degeneration." in: The British journal of ophthalmology, Vol. 91, Issue 12, pp. 1720-1, 2007 (PubMed).

Hill, Shukla, Tran et al.: "Inhibition of hypoxia inducible factor hydroxylases protects against renal ischemia-reperfusion injury." in: Journal of the American Society of Nephrology : JASN, Vol. 19, Issue 1, pp. 39-46, 2008 (PubMed).

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