HIF2A / HIF2 alpha (Sample Pack) antibody

Antigen: HIF2A / HIF2 alpha (Sample Pack)

Clonality: Polyclonal

Host: Please enquire

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA

Catalog no.: ABIN152189

Additional Information

Gene ID: 2034

Immunogen: NB 100-122 = Proprietary peptide derived from the mouse/human HIF-2a
NB 100-132 = Human HIF-2a amino acids 535-631
ABIN151927 = A fusion protein from mouse HIF-2 alpha, residues 355-438.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humansand endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functionsprimarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2gene, which is known to be selectively expressed in endothelial cells.

Specificity: These antibodies are specific for HIF-2a. Species Reactivity: NB 100-122 recognizes human, mouse, and rat HIF-2a. Other species have not beentested.NB 100-132 reacts with human and rat HIF-2 alpha. Results in mouse havebeen mixed (some + and some -). Other species have not been tested.ABIN151927reacts with both human and mouse proteins. Other species have not been tested.

Application Details

Application Notes: By Western blot, these antibodies recognize a band ~118 kDa, representing HIF-2a ininduced tissues and cells. The monoclonal has been used for immunohistochemistry. The polyclonal has also been used, but success has varied.Suggested working dilutions: *Western Blot - 1:500 -1:1,000+ **Immunohistochemistry - Varies (try 1:100~1,000)**Nuclear extracts should be used for Western analysis, if possible

Buffer: 1 vial each of NB 200-340 Rabbit anti-CARM1NB 200-341 Rabbit anti-CARM1 and NB 200-342 Rabbit anti-CARM1 Tris-citrate/phosphate pH 7-8 & 0.1% Sodium Azide

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Images

Western Blot analysis on normoxic and hypoxic nuclear rat cell lysates using ABIN151051, anti-HIF-2 alpha

anti-HIF2A / HIF2 alpha (Sample Pack) antibody (Image 2)

anti-HIF2A / HIF2 alpha (Sample Pack) antibody (Image 3)

Publications

Kuznetsova, Meller, Schnell et al.: "von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif and targets it for ubiquitination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2706-11, 2003 (PubMed).

Mascio, Olison, Ralphe et al.: "Myocardial vascular and metabolic adaptations in chronically anemic fetal sheep." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 289, Issue 6, pp. R1736-45, 2005 (PubMed).

Calvani, Rapisarda, Uranchimeg et al.: "Hypoxic induction of an HIF-1alpha-dependent bFGF autocrine loop drives angiogenesis in human endothelial cells." in: Blood, Vol. 107, Issue 7, pp. 2705-12, 2006 (PubMed).

Schultz, Fanburg, Beasley: "Hypoxia and hypoxia-inducible factor-1alpha promote growth factor-induced proliferation of human vascular smooth muscle cells." in: American journal of physiology. Heart and circulatory physiology, Vol. 290, Issue 6, pp. H2528-34, 2006 (PubMed).

Hui, Bauer, Striet et al.: "Calcium signaling stimulates translation of HIF-alpha during hypoxia." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 466-75, 2006 (PubMed).

Mizukami, Fujiki, Duerr et al.: "Hypoxic regulation of vascular endothelial growth factor through the induction of phosphatidylinositol 3-kinase/Rho/ROCK and c-Myc." in: The Journal of biological chemistry, Vol. 281, Issue 20, pp. 13957-63, 2006 (PubMed).

Nakamura, Martin, Jackson et al.: "Brain-derived neurotrophic factor activation of TrkB induces vascular endothelial growth factor expression via hypoxia-inducible factor-1alpha in neuroblastoma cells." in: Cancer research, Vol. 66, Issue 8, pp. 4249-55, 2006 (PubMed).

Shinojima, Oya, Takayanagi et al.: "Renal cancer cells lacking hypoxia inducible factor (HIF)-1alpha expression maintain vascular endothelial growth factor expression through HIF-2alpha." in: Carcinogenesis, Vol. 28, Issue 3, pp. 529-36, 2007 (PubMed).

Khacho, Mekhail, Pilon-Larose et al.: "Cancer-causing mutations in a novel transcription-dependent nuclear export motif of VHL abrogate oxygen-dependent degradation of hypoxia-inducible factor." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 302-14, 2007 (PubMed).

Inoue, Yanagi, Matsuura et al.: "Expression of hypoxia-inducible factor 1alpha and 2alpha in choroidal neovascular membranes associated with age-related macular degeneration." in: The British journal of ophthalmology, Vol. 91, Issue 12, pp. 1720-1, 2007 (PubMed).

Hill, Shukla, Tran et al.: "Inhibition of hypoxia inducible factor hydroxylases protects against renal ischemia-reperfusion injury." in: Journal of the American Society of Nephrology : JASN, Vol. 19, Issue 1, pp. 39-46, 2008 (PubMed).