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HIF2A / HIF2 alpha (Sample Pack) (AA 535-631) antibody

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AA 535-631
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Immunogen NB 100-122 = Proprietary peptide derived from the mouse/human HIF-2a. NB 100-132 = Human HIF-2a amino acids 535-631.
Kit Components NB100-132: HIF-2 alpha Antibody (ep190b), NB100-122: HIF-2 alpha Antibody
Specificity These antibodies are specific for HIF-2alpha.
Background Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humansand endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functionsprimarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2gene, which is known to be selectively expressed in endothelial cells.
Gene Symbol: EPAS1
Gene ID 2034
Application Notes By Western blot, these antibodies recognize a band ~118 kDa, representing HIF-2a in induced tissues and cells. The monoclonal has been used for immunohistochemistry. The polyclonal has also been used, but success has varied. Nuclear extracts should be used for Western analysis, if possible.
Restrictions For Research Use only
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-HIF2A / HIF2 alpha (Sample Pack) (AA 535-631) antibody (ABIN152189) Western Blot analysis on normoxic and hypoxic nuclear rat cell lysates using ABIN1510...
Immunohistochemistry (IHC) image for anti-HIF2A / HIF2 alpha (Sample Pack) (AA 535-631) antibody (ABIN152189) anti-HIF2A / HIF2 alpha (Sample Pack) (AA 535-631) antibody (Image 2)
Western Blotting (WB) image for anti-HIF2A / HIF2 alpha (Sample Pack) (AA 535-631) antibody (ABIN152189) anti-HIF2A / HIF2 alpha (Sample Pack) (AA 535-631) antibody (Image 3)
Product cited in: Hill, Shukla, Tran, Aragones, Cook, Carmeliet, Maxwell: "Inhibition of hypoxia inducible factor hydroxylases protects against renal ischemia-reperfusion injury." in: Journal of the American Society of Nephrology : JASN, Vol. 19, Issue 1, pp. 39-46, 2008

Khacho, Mekhail, Pilon-Larose, Payette, Lee: "Cancer-causing mutations in a novel transcription-dependent nuclear export motif of VHL abrogate oxygen-dependent degradation of hypoxia-inducible factor." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 302-14, 2007

Shinojima, Oya, Takayanagi, Mizuno, Shimizu, Murai: "Renal cancer cells lacking hypoxia inducible factor (HIF)-1alpha expression maintain vascular endothelial growth factor expression through HIF-2alpha." in: Carcinogenesis, Vol. 28, Issue 3, pp. 529-36, 2007

Inoue, Yanagi, Matsuura, Takahashi, Tamaki, Araie: "Expression of hypoxia-inducible factor 1alpha and 2alpha in choroidal neovascular membranes associated with age-related macular degeneration." in: The British journal of ophthalmology, Vol. 91, Issue 12, pp. 1720-1, 2007

Calvani, Rapisarda, Uranchimeg, Shoemaker, Melillo: "Hypoxic induction of an HIF-1alpha-dependent bFGF autocrine loop drives angiogenesis in human endothelial cells." in: Blood, Vol. 107, Issue 7, pp. 2705-12, 2006

Hui, Bauer, Striet, Schnell, Czyzyk-Krzeska: "Calcium signaling stimulates translation of HIF-alpha during hypoxia." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 466-75, 2006

Nakamura, Martin, Jackson, Beppu, Woo, Thiele: "Brain-derived neurotrophic factor activation of TrkB induces vascular endothelial growth factor expression via hypoxia-inducible factor-1alpha in neuroblastoma cells." in: Cancer research, Vol. 66, Issue 8, pp. 4249-55, 2006

Schultz, Fanburg, Beasley: "Hypoxia and hypoxia-inducible factor-1alpha promote growth factor-induced proliferation of human vascular smooth muscle cells." in: American journal of physiology. Heart and circulatory physiology, Vol. 290, Issue 6, pp. H2528-34, 2006

Mizukami, Fujiki, Duerr, Gala, Jo, Zhang, Chung: "Hypoxic regulation of vascular endothelial growth factor through the induction of phosphatidylinositol 3-kinase/Rho/ROCK and c-Myc." in: The Journal of biological chemistry, Vol. 281, Issue 20, pp. 13957-63, 2006

Mascio, Olison, Ralphe, Tomanek, Scholz, Segar: "Myocardial vascular and metabolic adaptations in chronically anemic fetal sheep." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 289, Issue 6, pp. R1736-45, 2005

Kuznetsova, Meller, Schnell, Nash, Ignacak, Sanchez, Conaway, Conaway, Czyzyk-Krzeska: "von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif and targets it for ubiquitination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2706-11, 2003