Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody

Antigen: Egl Nine Homolog 1 (C. Elegans) (EGLN1)

Synonyms: HPH2, PHD2, SM20, ECYT3, HIFPH2, ZMYND6, C1orf12, DKFZp761F179, Phd2, ORF13, SM-20, AI503754, zgc:92314, HPH-2, PHD-2, HIF-PH2, EGLN1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN152190

Additional Information

Alternative name: EGLN1 / PHD2 (Sample Pack)

Gene ID: 54583

Immunogen: The exact amino acid range is proprietary.
General Immunogen area:
NB 100-137 (LocusLink - 54583 Region - Internal)
NB 100-138 (LocusLink - 54583 Region - c-terminus)
NB 100-139 (LocusLink - 112399 Region - Internal)
NB 100-295 (LocusLink - 54681 Region - c-terminus)
NB 100-303 (LocusLink - 112399 Region - c-terminus)
NB 100-310 (LocusLink - 111298 Region - c-terminus)
ABIN250635 (mouse amino acid range 300-400).

Specificity: NB 100-137 - PHD2/EGLN1NB 100-138 - PHD2/EGLN1NB 100-139 - PHD3/EGLN3NB 100-295 - PHD4NB 100-303 - PHD3/EGLN3ABIN151776 - PHD1/EGLN2NB 100-2219 - PHD2/EGLN1. Species Reactivity: All: Homo sapiens. NB 100-137 does not react with mouse.NB 100-138 does not react with mouse and mixed results in rat.NB 100-139 does not react with mouse but reacts with rat.NB 100-295 does not react with mouse and is unknown in rat.NB 100-303 reacts with mouse.ABIN151776 reacts with mouse and rat.Other species have not been tested.

Application Details

Application Notes: These antibodies can be used for Western Blot at a dilution range of 1:500~1:1,000. Other applications, such as immunofluorescence, are not confirmed.

Purification: Affinity Purified

Buffer: 0.025 ml antibody per vial.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

Western Blot analysis of human PHD2, using ABIN151071. Samples: Recombinant FLAG-His-PHD1, PHD2 or PHD3 (10ng/lane) and whole cell lysate from HeLa cells.

Staining of lung, vascular endothelium using ABIN151071.

Western blot analysis of human PHD2, using ABIN151072. Samples: Recombinant FLAG-His-PHD1, PHD2 and PHD3 (10 ng/lane), HeLa whole cell lysate and MEFs.

Publications

Lu, Dalgard, Mohyeldin et al.: "Reversible inactivation of HIF-1 prolyl hydroxylases allows cell metabolism to control basal HIF-1." in: The Journal of biological chemistry, Vol. 280, Issue 51, pp. 41928-39, 2005 (PubMed).

Stiehl, Wirthner, Koeditz et al.: "Increased prolyl 4-hydroxylase domain proteins compensate for decreased oxygen levels. Evidence for an autoregulatory oxygen-sensing system." in: The Journal of biological chemistry, Vol. 281, Issue 33, pp. 23482-91, 2006 (PubMed).

McMahon, Charbonneau, Grandmont et al.: "Transforming growth factor beta1 induces hypoxia-inducible factor-1 stabilization through selective inhibition of PHD2 expression." in: The Journal of biological chemistry, Vol. 281, Issue 34, pp. 24171-81, 2006 (PubMed).

Kondo, Seo, Yoshizaki et al.: "EBV latent membrane protein 1 up-regulates hypoxia-inducible factor 1alpha through Siah1-mediated down-regulation of prolyl hydroxylases 1 and 3 in nasopharyngeal epithelial cells." in: Cancer research, Vol. 66, Issue 20, pp. 9870-7, 2006 (PubMed).

Choi, Kim, Kim et al.: "AKAP12 regulates human blood-retinal barrier formation by downregulation of hypoxia-inducible factor-1alpha." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 16, pp. 4472-81, 2007 (PubMed).

Baek, Liu, McDonald et al.: "Spermidine/spermine-N1-acetyltransferase 2 is an essential component of the ubiquitin ligase complex that regulates hypoxia-inducible factor 1alpha." in: The Journal of biological chemistry, Vol. 282, Issue 32, pp. 23572-80, 2007 (PubMed).

Colla, Tagliaferri, Morandi et al.: "The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesi" in: Blood, Vol. 110, Issue 13, pp. 4464-75, 2007 (PubMed).

Bex, Knauth, Dambacher et al.: "A yeast two-hybrid system reconstituting substrate recognition of the von Hippel-Lindau tumor suppressor protein." in: Nucleic acids research, Vol. 35, Issue 21, pp. e142, 2007 (PubMed).

Chang, Loh, Ceradini et al.: "Age decreases endothelial progenitor cell recruitment through decreases in hypoxia-inducible factor 1alpha stabilization during ischemia." in: Circulation, Vol. 116, Issue 24, pp. 2818-29, 2007 (PubMed).