Tumor Protein P53 Binding Protein 1 (TP53BP1) antibody

Details for Product No. ABIN152191
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Antigen
Synonyms TP53BP1, MGC160112, 53BP1, Tp53bp1, m53BP1, p202, Trp53bp1
Reactivity
Human, Mouse (Murine)
(140), (86), (49), (36), (12), (12), (1)
Host
Rabbit
(128), (12), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(91), (56), (42), (32), (30), (28), (23), (12), (5), (3), (2), (1)
Pubmed 4 references available
Quantity 0.1 mL
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Catalog No. ABIN152191
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Immunogen A synthetic peptide corresponding to a portion of human 53BP1 encoded in exon 11, 12 and 19. This antibody is a cocktail hybrid.
Cross-Reactivity (Details) Human has been tested in WB, IHC,ICC/IF, mouse has only been tested in ICC/IF.
Purification affinity purified
Alternative Name TP53BP1 (Cocktail)
Background P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putativesubstrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes tothe presumptive sites of damage, specifically, double-strand breaks. This also suggestsa role in DNA repair, maintaining genomic stability. Alternate Names: anti-53BP1 antibody, anti-53BP-1 antibody, anti-TP53BP1 antibody, anti-Tumor Proteinp53 Binding Protein 1 antibody.
Gene Symbol: TP53BP1
Gene ID 7158
UniProt Q12888
Application Notes This 53BP1 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot and Immunohistochemistry paraffin embedded sections. In Western blot a band was observed at ~220 kD. Irradiated lysates demonstrate a gel shift. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunohistochemistry 1:400, Immunohistochemistry-Paraffin 1:400, Western Blot 1:1000-1:10000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) antibody Figure 1 illustrates Western blot detection of 53BP1 (220 kDa) from untreated U2OS cell lysate using ABIN152191.
General Yanagawa, Funasaka, Tsutsumi et al.: "Regulation of phosphoglucose isomerase/autocrine motility factor activities by the poly(ADP-ribose) polymerase family-14." in: Cancer research, Vol. 67, Issue 18, pp. 8682-9, 2007 (PubMed).

Murga, Jaco, Fan et al.: "Global chromatin compaction limits the strength of the DNA damage response." in: The Journal of cell biology, Vol. 178, Issue 7, pp. 1101-8, 2007 (PubMed).

Toledo, Murga, Gutierrez-Martinez et al.: "ATR signaling can drive cells into senescence in the absence of DNA breaks." in: Genes & development, Vol. 22, Issue 3, pp. 297-302, 2008 (PubMed).

Pennarun, Granotier, Hoffschir et al.: "Role of ATM in the telomere response to the G-quadruplex ligand 360A." in: Nucleic acids research, Vol. 36, Issue 5, pp. 1741-54, 2008 (PubMed).

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