Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Antigen: Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A)

Synonyms: HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A, HIF-1a, HIF1a, hif-1a, hif1a

Clonality: Polyclonal

Host: Please enquire

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN152192

Additional Information

Alternative name: HIF1A / HIF1 alpha (Sample Pack)

Immunogen: NB 100-654 = Human HIF-1 alpha amino acids 432-528.NB 100-105 = Human HIF-1 alpha amino acids 432-528.NB 100-131 = Human HIF-1 alpha amino acids 329-530.NB 100-449 = Human HIF-1 alpha amino acids 775-826.ABIN151926 = Mouse HIF-1 alpha amino acids 530-825.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein that activates gene transcription in response to reducedcellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, hemeoxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is aheterodimer composed of HIF-1a and HIF-1b subunits. Both subunits are induced byhypoxia and rapidly decay upon return to normoxia and are basic-helix-loop-helix-PASproteins. Recent research indicates the ability to regulate hypoxia-inducible factors maybe related to tumor-related angiogenesis in certain cancers.

Specificity: Species Reactivity: NB 100-654 recognizes human, mouse and rat.NB 100-105 recognizes human, sheep, mouse, rat, porcine, rabbit, bovine and ferretHIF-1 alpha.NB 100-131 recognizes human, mouse and rat HIF-1 alpha.NB 100-449 recognizes human and mouse HIF-1 alpha . Prelimary results with mousetissue have been positive for IHC using mouse eye sections at 1:100 with high pHretrieval.ABIN151926 recognizes human and mouse HIF-1 alpha.

Application Details

Application Notes: Suggested working dilutions: Western Blot 1:500-1:2000,Immunohistochemistry-Paraffin 1:50-1:5000,Please See Datasheet

Buffer: NB 100-105: 0.05 ml Protein G purified mouse ascites. Protein concentration is 1.5mg/ml.NB 100-654: 0.025 ml affinity purified rabbit antisera Protein concentration is 1.2mg/ml.NB 100-131: 0.05 ml Protein G purified mouse ascites in PBS. Protein concentra

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Immunohistochemical staining of human glioblastoma multiforme utilizing ABIN151032.

Western analysis of 50ug cobalt chloride induced COS-7 nuclear extracts (NB800-PC26) using ABIN151032.

Publications

Su, Arakawa-Hoyt, Kan: "Adeno-associated viral vector-mediated hypoxia response element-regulated gene expression in mouse ischemic heart model." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 14, pp. 9480-5, 2002 (PubMed).

Winner, Koong, Rendon et al.: "Amplification of tumor hypoxic responses by macrophage migration inhibitory factor-dependent hypoxia-inducible factor stabilization." in: Cancer research, Vol. 67, Issue 1, pp. 186-93, 2007 (PubMed).

Jeon, Kim, Choi et al.: "Quercetin activates an angiogenic pathway, hypoxia inducible factor (HIF)-1-vascular endothelial growth factor, by inhibiting HIF-prolyl hydroxylase: a structural analysis of quercetin for inhibiting HIF-prolyl hydroxylase." in: Molecular pharmacology, Vol. 71, Issue 6, pp. 1676-84, 2007 (PubMed).