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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody
|Synonyms||HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A, HIF-1a, HIF1a, hif-1a, hif1a|
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Price||Product not available in this region.|
|Alternative name||HIF1A / HIF1 alpha (Sample Pack)|
|Immunogen||NB 100-654 = Human HIF-1 alpha amino acids 432-528.NB 100-105 = Human HIF-1 alpha amino acids 432-528.NB 100-131 = Human HIF-1 alpha amino acids 329-530.NB 100-449 = Human HIF-1 alpha amino acids 775-826.ABIN151926 = Mouse HIF-1 alpha amino acids 530-825.|
|Cross-Reactivity||Human, Mouse (Murine), Rat (Rattus)|
|Description||Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein that activates gene transcription in response to reducedcellular 02 concentration. HIF-1 activates the transcription of EPO, VEGF, iNOS, hemeoxygenase 1 and several other critical intracellular responses to hypoxia. HIF-1 is aheterodimer composed of HIF-1a and HIF-1b subunits. Both subunits are induced byhypoxia and rapidly decay upon return to normoxia and are basic-helix-loop-helix-PASproteins. Recent research indicates the ability to regulate hypoxia-inducible factors maybe related to tumor-related angiogenesis in certain cancers.|
|Specificity||Species Reactivity: NB 100-654 recognizes human, mouse and rat.NB 100-105 recognizes human, sheep, mouse, rat, porcine, rabbit, bovine and ferretHIF-1 alpha.NB 100-131 recognizes human, mouse and rat HIF-1 alpha.NB 100-449 recognizes human and mouse HIF-1 alpha . Prelimary results with mousetissue have been positive for IHC using mouse eye sections at 1:100 with high pHretrieval.ABIN151926 recognizes human and mouse HIF-1 alpha.|
|Application Notes||Suggested working dilutions: Western Blot 1:500-1:2000,Immunohistochemistry-Paraffin 1:50-1:5000,Please See Datasheet|
|Buffer||NB 100-105: 0.05 ml Protein G purified mouse ascites. Protein concentration is 1.5mg/ml.NB 100-654: 0.025 ml affinity purified rabbit antisera Protein concentration is 1.2mg/ml.NB 100-131: 0.05 ml Protein G purified mouse ascites in PBS. Protein concentra|
|Storage||Store at 4C. Do not freeze.|
|Restrictions||For Research Use only|
Su, Arakawa-Hoyt, Kan: "Adeno-associated viral vector-mediated hypoxia response element-regulated gene expression in mouse ischemic heart model." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 14, pp. 9480-5, 2002 (PubMed).
Winner, Koong, Rendon et al.: "Amplification of tumor hypoxic responses by macrophage migration inhibitory factor-dependent hypoxia-inducible factor stabilization." in: Cancer research, Vol. 67, Issue 1, pp. 186-93, 2007 (PubMed).
Jeon, Kim, Choi et al.: "Quercetin activates an angiogenic pathway, hypoxia inducible factor (HIF)-1-vascular endothelial growth factor, by inhibiting HIF-prolyl hydroxylase: a structural analysis of quercetin for inhibiting HIF-prolyl hydroxylase." in: Molecular pharmacology, Vol. 71, Issue 6, pp. 1676-84, 2007 (PubMed).