APEX1 / REF-1 (2 set) antibody

Antigen: APEX1 / REF-1 (2 set)

Clonality: Polyclonal

Host: Please enquire

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN152196

Additional Information

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: We know how important it is to your research to have access to antibodies specific foryour model. As it is often impossible to know which antibody will work best for a specificsample and application, we now offer SuperNovus Packs. These packs include differentantibodies to the same protein to give you the ability to decide for yourself. TheSuperNovus Packs are all offered at a discount price compared to purchasing eachantibody individually.The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for therepair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitatingthe DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and othertranscription factors. APE/ref-1 has also been shown to control p53 activity throughredox alteration. APE is linked to apoptosis, associated with thioredoxin, and alteredlevels of APE/ref-1 have been found in some cancers. APE appears to form a uniquelink between the DNA base excision pathway, oxidative signaling, transcriptionregulation, cancer and cell-cycle control.

Specificity: Species Reactivity: See individual datasheets.

Application Details

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

Immunohistochemical staining of APE-ref-1 in prostate cancer, using ABIN151028

Western analysis of APE-ref-1 in HeLa and 3T3 cell lysates, using ABIN151028

Publications

Evans, Limp-Foster, Kelley: "Going APE over ref-1." in: Mutation research, Vol. 461, Issue 2, pp. 83-108, 2001 (PubMed).

Zou, Luo, Reed et al.: "Ape1 regulates hematopoietic differentiation of embryonic stem cells through its redox functional domain." in: Blood, Vol. 109, Issue 5, pp. 1917-22, 2007 (PubMed).

Briegert, Kaina: "Human monocytes, but not dendritic cells derived from them, are defective in base excision repair and hypersensitive to methylating agents." in: Cancer research, Vol. 67, Issue 1, pp. 26-31, 2007 (PubMed).