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APEX1 / REF-1 (2 Set) antibody

Details for Product No. ABIN152196, Supplier: Log in to see
Human, Mouse (Murine), Rat (Rattus)
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Specificity These antibodies are specific for APE1.
Cross-Reactivity (Details) Other species have not been tested.
Background The mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is responsible for therepair of AP sites in DNA. In addition, this enzyme functions as a redox factor facilitatingthe DNA binding capability of FOS, JUN, NfkB, HIF-1 alpha, Pax-5, Pax-8 and othertranscription factors. APE/ref-1 has also been shown to control p53 activity throughredox alteration. APE is linked to apoptosis, associated with thioredoxin, and alteredlevels of APE/ref-1 have been found in some cancers. APE appears to form a uniquelink between the DNA base excision pathway, oxidative signaling, transcriptionregulation, cancer and cell-cycle control.
Gene Symbol: APEX1
Gene ID 328
Application Notes The antibodies included in this pack are useful for Western blot, immunohistochemistry and immunoprecipitation.
Restrictions For Research Use only
Buffer Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-APEX1 / REF-1 (2 Set) antibody (ABIN152196) Immunohistochemical staining of APE-ref-1 in prostate cancer, using ABIN151028
Western Blotting (WB) image for anti-APEX1 / REF-1 (2 Set) antibody (ABIN152196) Western analysis of APE-ref-1 in HeLa and 3T3 cell lysates, using ABIN151028
Product cited in: Briegert, Kaina: "Human monocytes, but not dendritic cells derived from them, are defective in base excision repair and hypersensitive to methylating agents." in: Cancer research, Vol. 67, Issue 1, pp. 26-31, 2007

Zou, Luo, Reed, Kelley, Yoder: "Ape1 regulates hematopoietic differentiation of embryonic stem cells through its redox functional domain." in: Blood, Vol. 109, Issue 5, pp. 1917-22, 2007

Background publications Evans, Limp-Foster, Kelley: "Going APE over ref-1." in: Mutation research, Vol. 461, Issue 2, pp. 83-108, 2001