Profilin 1 (PFN1) antibody

Details for Product No. ABIN152302, Supplier: Log in to see
Antigen
  • GmPRO1
  • GmPRO2
  • PRO1
  • Profilin-1
  • profilin-1
  • profilin
  • MGC130883
  • Profilin1
  • XProfilin1
  • sb:cb813
  • wu:fa91a03
  • ALS18
  • Pfn
  • F6F22.21
  • F6F22_21
  • PFN1
  • PROFILIN 1
  • profilin 1
  • profilin
  • profilin 1
  • profilin 1-like
  • profilin homolog1
  • profilin-1-like
  • Protein PFN-1
  • PRO2
  • PFN1
  • pfn1
  • Pfn1
  • PRF1
  • LOC100350198
  • prf1
  • LOC607397
  • pfn-1
Alternatives
anti-Human Profilin 1 antibody for Immunofluorescence (fixed cells)
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
92
58
55
25
4
3
3
3
2
1
1
1
1
1
1
1
Host
Rabbit
92
12
Clonality
Polyclonal
Conjugate
Un-conjugated
10
9
6
2
2
2
2
2
2
1
1
1
1
1
Application
Western Blotting (WB)
86
47
31
26
12
12
12
4
4
3
2
1
1
Options
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Immunogen Porcine and human platelet Profilin 1
Specificity This antibody is specific for profilin I protein but also reacts with profilin II.
Purification Immunogen affinity purified
Alternative Name Profilin 1 (PFN1 Antibody Abstract)
Background Gene Symbol: PFN1
Gene ID 5216
Research Area Neurology, Microfilaments, Cytoskeleton
Pathways Regulation of Actin Filament Polymerization, Tube Formation, Maintenance of Protein Location
Application Notes Western Blot 0.1-0.2 μg/mLA band should be seen at ~12-15 kDa, in Western blotting. Use tricine gels (13 % acrylamide) or 15 % Laemmli gels. **The given dilutions refer to the analysis of mammalian cells and tissues with intermediate levels of profilin expression.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Profilin 1 Antibody Western Blot Protocol:
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk and 1 % BSA in TBS for 1 hour.
. Dilute the rabbit anti-profilin primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Profilin 1 (PFN1) antibody (ABIN152302) anti-Profilin 1 (PFN1) antibody
Product cited in: Stoeckelhuber, Scherer, Janssen, Slotta-Huspenina, Loeffelbein, Rohleder, Nieberler, Hasler, Kesting: "The human submandibular gland: immunohistochemical analysis of SNAREs and cytoskeletal proteins." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 60, Issue 2, pp. 110-20, 2012 (PubMed).

Evans, Walker: "Endothelin-1 mobilizes profilin-1-bound PIP2 in cardiac muscle." in: Experimental biology and medicine (Maywood, N.J.), Vol. 231, Issue 6, pp. 882-7, 2006 (PubMed).

Giesemann, Rathke-Hartlieb, Rothkegel, Bartsch, Buchmeier, Jockusch, Jockusch: "A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems." in: The Journal of biological chemistry, Vol. 274, Issue 53, pp. 37908-14, 2000 (PubMed).

Salazar, Bell, Davis: "Coordinate induction of the actin cytoskeletal regulatory proteins gelsolin, vasodilator-stimulated phosphoprotein, and profilin during capillary morphogenesis in vitro." in: Experimental cell research, Vol. 249, Issue 1, pp. 22-32, 1999 (PubMed).

Hauser, Knobeloch, Eigenthaler, Gambaryan, Krenn, Geiger, Glazova, Rohde, Horak, Walter, Zimmer: "Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 14, pp. 8120-5, 1999 (PubMed).