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Profilin 1 (PFN1) antibody

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Synonyms GmPRO1, GmPRO2, PRO1, Profilin-1, profilin-1, profilin, MGC130883, Profilin1, XProfilin1, sb:cb813, wu:fa91a03, ALS18, Pfn, F6F22.21, F6F22_21, PFN1, PROFILIN 1, profilin 1
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(71), (32), (28), (9), (1), (1), (1), (1)
(71), (6)
(8), (7), (6), (1), (1), (1), (1)
Western Blotting (WB)
(61), (35), (23), (17), (7), (5), (4), (2), (2), (2)
Pubmed 3 references available
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Quantity 0.1 mL
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Immunogen Porcine and human platelet profili
Specificity NB200-162 is specific for profilin I protein but also reacts with profilin II.
Purification affinity purified
Alternative Name Profilin-1 / PFN1 (PFN1 Antibody Abstract)
Background Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline bindingprotein that plays a role both in signal transduction pathways and actin filamentdynamics. There are two mammalian profilins with similar biochemical properties butdifferent expression patterns. Whereasprofilin I appears to be highly expressed in most tissues except for skeletal muscle,profilin II ispredominantly expressed in brain and at lower levels also in skeletal muscle, uterus andkidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamicmembrane areas like the leading edge and ruffling membranes. Alternate Names: anti-Pfn antibody, anti-PFN1 antibody.
Gene Symbol: PFN1
Gene ID 5216, 18643, 64303
Application Notes A band should be seen at ~12-15 kDa, in Western blotting. Use tricine gels (13% acrylamide) or 15% Laemmli gels. **The given dilutions refer to the analysis of mammalian cells and tissues with intermediate levels of profilin expression.
Recommended dilutions: Western Blot 0.1-0.2 µg/mL
Protocol Protocol specific for Profilin 1 Antibody Western Blot Protocol:
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk and 1 % BSA in TBS for 1 hour.
. Dilute the rabbit anti-profilin primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS, containing 1 mg/ml BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Western Blotting (WB) image for anti-Profilin 1 (PFN1) antibody (ABIN152302) anti-Profilin 1 (PFN1) antibody
Product cited in: Giesemann, Rathke-Hartlieb, Rothkegel et al.: "A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems." in: The Journal of biological chemistry, Vol. 274, Issue 53, pp. 37908-14, 2000 (PubMed).

Background publications Hauser, Knobeloch, Eigenthaler et al.: "Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 14, pp. 8120-5, 1999 (PubMed).

Salazar, Bell, Davis: "Coordinate induction of the actin cytoskeletal regulatory proteins gelsolin, vasodilator-stimulated phosphoprotein, and profilin during capillary morphogenesis in vitro." in: Experimental cell research, Vol. 249, Issue 1, pp. 22-32, 1999 (PubMed).

Catalog No. ABIN152302
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