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HPGD antibody (Hydroxyprostaglandin Dehydrogenase 15-(NAD))

Details for Product anti-HPGD Antibody No. ABIN152315, Supplier: Log in to see
Antigen
  • MGC80546
  • MGC79454
  • 15-PGDH
  • AV026552
  • PGDH
  • PGDH1
  • PHOAR1
  • SDR36C1
  • hydroxyprostaglandin dehydrogenase 15-(NAD)
  • 15-hydroxyprostaglandin dehydrogenase
  • hydroxyprostaglandin dehydrogenase 15 (NAD)
  • HPGD
  • hpgd
  • pgdh
  • PTRG_03214
  • VDBG_09957
  • Hpgd
Reactivity
Human, Mouse (Murine), Rat (Rattus)
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22
6
4
4
4
3
3
2
1
1
1
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1
Host
Rabbit
72
32
23
Clonality
Polyclonal
Conjugate
This HPGD antibody is un-conjugated
6
6
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
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43
34
32
18
13
13
7
7
5
3
2
1
1
1
Options
Supplier
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Immunogen Purified type I human placental 15-PGDH protein [UniProt# P15428]
Purification Protein A purified
Alternative Name 15-PGDH/HPGD (HPGD Antibody Abstract)
Background Gene Symbol: HPGD
Gene ID 3248
UniProt P15428
Application Notes Western Blot 1:5000-1:6000, Simple Western 1:500, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:200, Immunohistochemistry-FrozenThis 15-PGDH antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot. In Western blot bands may be seen at 29 kDa and 58 kDa (homodimer) depending on the lysate used. In ICC/IF cytoplasmic staining was observed in HeLa cells. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot Protocols specific for 15-PGDH Antibody Western Blot I (LoVo lysates)
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 20ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS for 1 hour.
. Dilute the rabbit anti-15-PGDH primary antibody in blocking buffer and incubate overnight at 4C.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Amersham's ECL is the standard reagent used).**Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.Western Blot II (A549 lysates)
. Cell lysates are prepared in RIPA buffer (50 mM 50 mM Tris-HCL/1 % Nonidet P-40/0.25 % Na-deoxycholate/150 mM NaCl/1 mM EDTA/1 mM PMSF) supplemented with a protease inhibitor mixture (Roche Applied Sciences).
. They are then separated on 10 % or 12 % SDS/PAGE (30-150 µg per lane) and transferred to a Immobilon PVDF membrane (Millipore).
. The membrane is blocked with 5 % NFDM in TBS-T (TBS + 0.1 % Tween-20).
. The membrane is then probed with the diluted anti-PGDH antibody , diluted in blocking buffer at RT for 1 hour.
. The membrane is washed 3 times with TBS-T.
. The membrane is then incubated with a biotinylated goat anti-rabbit IgG (diluted as per manufacturer's guidelines in blocking buffer) at RT for 1 hour.
. The membrane was washed extensively.
. The membrane is then incubated with an HRP-conjugated streptavidin (1:2,000) complex at RT for 1 hour.
. The membrane is washed extensively.
. The membrane is developed using and ECL detection system.
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-HPGD antibody (Hydroxyprostaglandin Dehydrogenase 15-(NAD)) (ABIN152315) anti-Hydroxyprostaglandin Dehydrogenase 15-(NAD) (HPGD) antibody
Simple Western (SimWes) image for anti-HPGD antibody (Hydroxyprostaglandin Dehydrogenase 15-(NAD)) (ABIN152315) Simple Western: 15-PGDH/HPGD Antibody [ABIN152315] - Simple Western lane view shows a...
Western Blotting (WB) image for anti-HPGD antibody (Hydroxyprostaglandin Dehydrogenase 15-(NAD)) (ABIN152315) Western Blot: 15-PGDH/HPGD Antibody [ABIN152315] - Western blot analysis of 15-PGDH i...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-HPGD antibody (Hydroxyprostaglandin Dehydrogenase 15-(NAD)) (ABIN152315) Immunohistochemistry-Paraffin: 15-PGDH/HPGD Antibody [ABIN152315] - IHC analysis of 1...
Immunofluorescence (IF) image for anti-HPGD antibody (Hydroxyprostaglandin Dehydrogenase 15-(NAD)) (ABIN152315) Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody [ABIN152315] - 15-PGDH ...
Product cited in: Miyagishi, Kosuge, Takano, Endo, Nango, Yamagata-Murayama, Hirose, Kano, Tanaka, Ishige, Ito: "Increased Expression of 15-Hydroxyprostaglandin Dehydrogenase in Spinal Astrocytes During Disease Progression in a Model of Amyotrophic Lateral Sclerosis." in: Cellular and molecular neurobiology, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry (Frozen Sections)

Mehdawi, Prasad, Ehrnström, Andersson, Sjölander: "Non-canonical WNT5A signaling up-regulates the expression of the tumor suppressor 15-PGDH and induces differentiation of colon cancer cells." in: Molecular oncology, Vol. 10, Issue 9, pp. 1415-1429, 2016 (PubMed). Further details: Western Blotting,Immunohistochemistry

de Hair, Leclerc, Newsum, Maijer, van de Sande, Ramwadhdoebe, van Schaardenburg, van Baarsen, Korotkova, Gerlag, Tak, Jakobsson: "Expression of Prostaglandin E2 Enzymes in the Synovium of Arthralgia Patients at Risk of Developing Rheumatoid Arthritis and in Early Arthritis Patients." in: PLoS ONE, Vol. 10, Issue 7, pp. e0133669, 2015 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Hsiao, Thatcher, Colas, Serhan, Phipps, Sime: "Resolvin D1 Reduces Emphysema and Chronic Inflammation." in: The American journal of pathology, Vol. 185, Issue 12, pp. 3189-201, 2015 (PubMed).

Ryu, Myung, Park, Yang, Song, Jeong, Lee, Song, Kim, Lee, Park, Fink, Markowitz, Jung, Kim, Ye, Byeon, Jung, Yang, Kim: "Inhibition of 15-hydroxyprostaglandin dehydrogenase by Helicobacter pylori in human gastric carcinogenesis." in: Cancer prevention research (Philadelphia, Pa.), Vol. 6, Issue 4, pp. 349-59, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Chung, Lim, Kim, Shin, Chung, Choi, Kim, Kim, Park, Chang: "High-fat diet-induced renal cell apoptosis and oxidative stress in spontaneously hypertensive rat are ameliorated by fenofibrate through the PPARα-FoxO3a-PGC-1α pathway." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 27, Issue 6, pp. 2213-25, 2012 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Gheorghe, Sadique, Leclerc, Idborg, Wobst, Catrina, Jakobsson, Korotkova: "Limited effect of anti-rheumatic treatment on 15-prostaglandin dehydrogenase in rheumatoid arthritis synovial tissue." in: Arthritis research & therapy, Vol. 14, Issue 3, pp. R121, 2012 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Subbaramaiah, Howe, Zhou, Yang, Hudis, Kopelovich, Dannenberg: "Pioglitazone, a PPARγ agonist, suppresses CYP19 transcription: evidence for involvement of 15-hydroxyprostaglandin dehydrogenase and BRCA1." in: Cancer prevention research (Philadelphia, Pa.), Vol. 5, Issue 10, pp. 1183-94, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Kang, Kim, Moon, Lim, Kim: "Prognostic implication of 15-hydroxyprostaglandin dehydrogenase down-regulation in patients with colorectal cancer." in: Journal of the Korean Society of Coloproctology, Vol. 28, Issue 5, pp. 253-8, 2012 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Judson, Miyaki, Kekatpure, Du, Gilleaudeau, Sullivan-Whalen, Mohebati, Nair, Boyle, Granstein, Subbaramaiah, Krueger, Dannenberg: "UV radiation inhibits 15-hydroxyprostaglandin dehydrogenase levels in human skin: evidence of transcriptional suppression." in: Cancer prevention research (Philadelphia, Pa.), Vol. 3, Issue 9, pp. 1104-11, 2010 (PubMed). (Sample species: Human). Further details: Western Blotting

Hughes, Otani, Yang, Newman, Yantiss, Altorki, Port, Yan, Markowitz, Mazumdar, Tai, Subbaramaiah, Dannenberg: "NAD+-dependent 15-hydroxyprostaglandin dehydrogenase regulates levels of bioactive lipids in non-small cell lung cancer." in: Cancer prevention research (Philadelphia, Pa.), Vol. 1, Issue 4, pp. 241-9, 2009 (PubMed).

Thirumangalakudi, Rao, Grammas: "Involvement of PGE2 and PGDH but not COX-2 in thrombin-induced cortical neuron apoptosis." in: Neuroscience letters, Vol. 452, Issue 2, pp. 172-5, 2009 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Miyaki, Yang, Tai, Subbaramaiah, Dannenberg: "Bile acids inhibit NAD+-dependent 15-hydroxyprostaglandin dehydrogenase transcription in colonocytes." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 297, Issue 3, pp. G559-66, 2009 (PubMed).

Backlund, Mann, Holla, Shi, Daikoku, Dey, DuBois: "Repression of 15-hydroxyprostaglandin dehydrogenase involves histone deacetylase 2 and snail in colorectal cancer." in: Cancer research, Vol. 68, Issue 22, pp. 9331-7, 2008 (PubMed).

Michelet, Colombe, Gautier, Gaillard, Benech, Pereira, Boulle, Dalko-Csiba, Rozot, Neuwels, Bernard: "Expression of NAD+ dependent 15-hydroxyprostaglandin dehydrogenase and protection of prostaglandins in human hair follicle." in: Experimental dermatology, Vol. 17, Issue 10, pp. 821-8, 2008 (PubMed). (Sample species: Human). Further details: Western Blotting

Mann, Backlund, Buchanan, Daikoku, Holla, Rosenberg, Dey, DuBois: "Repression of prostaglandin dehydrogenase by epidermal growth factor and snail increases prostaglandin E2 and promotes cancer progression." in: Cancer research, Vol. 66, Issue 13, pp. 6649-56, 2006 (PubMed).

Backlund, Mann, Holla, Buchanan, Tai, Musiek, Milne, Katkuri, DuBois: "15-Hydroxyprostaglandin dehydrogenase is down-regulated in colorectal cancer." in: The Journal of biological chemistry, Vol. 280, Issue 5, pp. 3217-23, 2005 (PubMed).

Ding, Tong, Liu, Moscow, Tai: "NAD+-linked 15-hydroxyprostaglandin dehydrogenase (15-PGDH) behaves as a tumor suppressor in lung cancer." in: Carcinogenesis, Vol. 26, Issue 1, pp. 65-72, 2004 (PubMed).

Tong, Tai: "Synergistic induction of the nicotinamide adenine dinucleotide-linked 15-hydroxyprostaglandin dehydrogenase by an androgen and interleukin-6 or forskolin in human prostate cancer cells." in: Endocrinology, Vol. 145, Issue 5, pp. 2141-7, 2004 (PubMed).

Yan, Rerko, Platzer, Dawson, Willis, Tong, Lawrence, Lutterbaugh, Lu, Willson, Luo, Hensold, Tai, Wilson, Markowitz: "15-Hydroxyprostaglandin dehydrogenase, a COX-2 oncogene antagonist, is a TGF-beta-induced suppressor of human gastrointestinal cancers." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 50, pp. 17468-73, 2004 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry