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DDX6 antibody (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6) (AA 1-50)

Details for Product anti-DDX6 Antibody No. ABIN152327, Supplier: Log in to see
Antigen
  • si:ch211-147p17.1
  • P54H
  • Xp54
  • hlr2
  • me31b
  • p54
  • rck
  • HLR2
  • P54
  • RCK
  • p54h
  • RGD1564560
  • 1110001P04Rik
  • E230023J21Rik
  • mRCK/P54
  • DEAD (Asp-Glu-Ala-Asp) box helicase 6
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
  • DDX6
  • ddx6
  • Ddx6
Epitope
AA 1-50
37
16
15
13
9
6
5
3
2
2
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
103
21
18
6
3
2
1
1
1
1
Host
Rabbit
74
17
12
Clonality
Polyclonal
Conjugate
This DDX6 antibody is un-conjugated
6
5
5
5
5
5
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
95
54
29
13
7
7
4
2
1
1
Options
Supplier
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Immunogen The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry P26196 (GeneID 1656).
Specificity This antibody is specific for human DDX6 protein.
Cross-Reactivity (Details) Based on 100% sequence identity, this antibody is predicted to react with Rat, Chicken, Turkey, Bovine, Dog, Horse, Rabbit, Guinea pig_10141, Pig, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Green anole, White-tufted-ear marmoset, Chinese hamster,Naked mole rat.
Purification Immunogen affinity purified
Alternative Name DDX6 (DDX6 Antibody Abstract)
Background Gene Symbol: DDX6
Gene ID 1656
UniProt P26196
Research Area DNA/RNA, Chromatin and Nuclear Signaling
Application Notes Western Blot 1:5000-1:15000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:50-1:500, Immunoprecipitation 2-5 μg/mg lysate, Immunohistochemistry-Paraffin 1:100-1:500Immunocytochemistry/Immunofluorescence was reported in scientific literature. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer TBS and 0.1 % BSA
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-DDX6 antibody (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6) (AA 1-50) (ABIN152327) Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DDX6 antibody (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6) (AA 1-50) (ABIN152327) Immunohistochemistry-Paraffin: DDX6 Antibody [ABIN152327] - Human colon carcinoma (le...
Product cited in: Somasekharan, El-Naggar, Leprivier, Cheng, Hajee, Grunewald, Zhang, Ng, Delattre, Evdokimova, Wang, Gleave, Sorensen: "YB-1 regulates stress granule formation and tumor progression by translationally activating G3BP1." in: The Journal of cell biology, Vol. 208, Issue 7, pp. 913-29, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

de Vries, Naarmann-de Vries, Urlaub, Lue, Bernhagen, Ostareck, Ostareck-Lederer: "Identification of DEAD-box RNA helicase 6 (DDX6) as a cellular modulator of vascular endothelial growth factor expression under hypoxia." in: The Journal of biological chemistry, Vol. 288, Issue 8, pp. 5815-27, 2013 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Marnef, Weil, Standart: "RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor." in: Molecular biology of the cell, Vol. 23, Issue 1, pp. 213-24, 2012 (PubMed).

Crist, Montarras, Buckingham: "Muscle satellite cells are primed for myogenesis but maintain quiescence with sequestration of Myf5 mRNA targeted by microRNA-31 in mRNP granules." in: Cell stem cell, Vol. 11, Issue 1, pp. 118-26, 2012 (PubMed).

Ernoult-Lange, Baconnais, Harper, Minshall, Souquere, Boudier, Bénard, Andrey, Pierron, Kress, Standart, le Cam, Weil: "Multiple binding of repressed mRNAs by the P-body protein Rck/p54." in: RNA (New York, N.Y.), Vol. 18, Issue 9, pp. 1702-15, 2012 (PubMed).

Novotny, Podolská, Blazíková, Valásek, Svoboda, Stanek: "Nuclear LSm8 affects number of cytoplasmic processing bodies via controlling cellular distribution of Like-Sm proteins." in: Molecular biology of the cell, Vol. 23, Issue 19, pp. 3776-85, 2012 (PubMed).

Nihei, Ito, Suzuki: "Roles of ataxin-2 in pathological cascades mediated by TAR DNA-binding protein 43 (TDP-43) and Fused in Sarcoma (FUS)." in: The Journal of biological chemistry, Vol. 287, Issue 49, pp. 41310-23, 2012 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Cougot, Cavalier, Thomas, Gillet: "The dual organization of P-bodies revealed by immunoelectron microscopy and electron tomography." in: Journal of molecular biology, Vol. 420, Issue 1-2, pp. 17-28, 2012 (PubMed).

Totaro, Renzi, La Fata, Mattioli, Raabe, Urlaub, Achsel: "The human Pat1b protein: a novel mRNA deadenylation factor identified by a new immunoprecipitation technique." in: Nucleic acids research, Vol. 39, Issue 2, pp. 635-47, 2011 (PubMed).

Saito, Kondo, Matsushita: "MicroRNA 130 family regulates the hypoxia response signal through the P-body protein DDX6." in: Nucleic acids research, Vol. 39, Issue 14, pp. 6086-99, 2011 (PubMed).

Hölzel, Orban, Hochstatter, Rohrmoser, Harasim, Malamoussi, Kremmer, Längst, Eick: "Defects in 18 S or 28 S rRNA processing activate the p53 pathway." in: The Journal of biological chemistry, Vol. 285, Issue 9, pp. 6364-70, 2010 (PubMed). (Sample species: Human).

Emara, Ivanov, Hickman, Dawra, Tisdale, Kedersha, Hu, Anderson: "Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly." in: The Journal of biological chemistry, Vol. 285, Issue 14, pp. 10959-68, 2010 (PubMed).

Savas, Ma, Deinhardt, Culver, Restituito, Wu, Belasco, Chao, Tanese: "A role for huntington disease protein in dendritic RNA granules." in: The Journal of biological chemistry, Vol. 285, Issue 17, pp. 13142-53, 2010 (PubMed).

Kemler, Meehan, Poeschla: "Live-cell coimaging of the genomic RNAs and Gag proteins of two lentiviruses." in: Journal of virology, Vol. 84, Issue 13, pp. 6352-66, 2010 (PubMed).

Jangra, Yi, Lemon: "DDX6 (Rck/p54) is required for efficient hepatitis C virus replication but not for internal ribosome entry site-directed translation." in: Journal of virology, Vol. 84, Issue 13, pp. 6810-24, 2010 (PubMed).

Marnef, Maldonado, Bugaut, Balasubramanian, Kress, Weil, Standart: "Distinct functions of maternal and somatic Pat1 protein paralogs." in: RNA (New York, N.Y.), Vol. 16, Issue 11, pp. 2094-107, 2010 (PubMed).

Otsuka, Kedersha, Schoenberg: "Identification of a cytoplasmic complex that adds a cap onto 5'-monophosphate RNA." in: Molecular and cellular biology, Vol. 29, Issue 8, pp. 2155-67, 2009 (PubMed).

Taddeo, Zhang, Roizman: "The virion-packaged endoribonuclease of herpes simplex virus 1 cleaves mRNA in polyribosomes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 29, pp. 12139-44, 2009 (PubMed).

Souquere, Mollet, Kress, Dautry, Pierron, Weil: "Unravelling the ultrastructure of stress granules and associated P-bodies in human cells." in: Journal of cell science, Vol. 122, Issue Pt 20, pp. 3619-26, 2009 (PubMed).

Ernoult-Lange, Wilczynska, Harper, Aigueperse, Dautry, Kress, Weil: "Nucleocytoplasmic traffic of CPEB1 and accumulation in Crm1 nucleolar bodies." in: Molecular biology of the cell, Vol. 20, Issue 1, pp. 176-87, 2009 (PubMed).