DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6) antibody

Details for Product No. ABIN152327
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Antigen
Synonyms si:ch211-147p17.1, P54H, Xp54, hlr2, me31b, p54, rck, HLR2, P54, RCK, p54h, RGD1564560, 1110001P04Rik, E230023J21Rik, mRCK/P54
Reactivity
Human
(45), (10), (9), (3), (1), (1), (1)
Host
Rabbit
(39), (5), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(45), (23), (13), (8), (4), (4), (2), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mL
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Catalog No. ABIN152327
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Immunogen The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry P26196 (GeneID 1656).
Specificity NB200-191 is specific for human DDX6 protein.
Cross-Reactivity (Details) Based on 100% sequence identity, this antibody is predicted to react with Rat, Chicken, Turkey, Bovine, Dog, Horse, Rabbit, Guinea pig_10141, Pig, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Green anole, White-tufted-ear marmoset, Chinese hamster,Naked mole rat.
Purification affinity purified
Alternative Name DEAD box protein 6
Background DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), areputative RNA helicases. They are implicated in a number of cellular processes involvingalteration of RNA secondary structure such as translation initiation, nuclear andmitochondrial splicing, and ribosome and spliceosome assembly. Based on theirdistribution patterns, some members of this family are believed to be involved inembryogenesis, spermatogenesis, and cellular growth and division. This gene encodesa DEAD box protein. It may contribute to the cell proliferation and carcinogenesis. Alternate Names: anti-DDX6 antibody, anti-DDX-6 antibody, anti-DEAD (Asp-Glu-Ala-Asp) boxpolypeptide 6 antibody, anti-HLR2 antibody, anti-HLR-2 Helicase- RNA-Nuclear 2antibody, anti-P54 RNA Helicase antibody, anti-RCK antibody, anti-Breakpoint in RC-K8B-cell lymphoma cell line antibody.
Gene Symbol: DDX6
Gene ID 1656
Application Notes Immunocytochemistry/Immunofluorescence was reported in scientific literature. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:50-1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:100-1:500, Immunoprecipitation 2-5 µg/mg lysate, Western Blot 1:5000-1:15000
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer Tris-buffered Saline and 0.1 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6) antibody Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody ABIN152327 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody ABIN152328, but not with DDX6 Ab, which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).
General Nonhoff, Ralser, Welzel et al.: "Ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6 and interferes with P-bodies and stress granules." in: Molecular biology of the cell, Vol. 18, Issue 4, pp. 1385-96, 2007 (PubMed).

Validation Images
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