DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6) antibody

Details for Product No. ABIN152327
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Antigen
Synonyms HLR2, P54, RCK, RGD1564560, 1110001P04Rik, E230023J21Rik, mRCK/P54, p54, rck, p54h, si:ch211-147p17.1, P54H, Xp54, hlr2, me31b
Reactivity
Human
(29), (10), (9), (3), (1), (1), (1)
Host
Rabbit
(24), (5), (3)
Clonality
Polyclonal
Application
Immunoprecipitation (IP), Western Blotting (WB)
(31), (11), (7), (5), (2), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN152327
Quantity 20 µg
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Immunogen The epitope recognized by this antibody maps to a region between residues 1 and 50 ofhuman DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering givenSwiss-Prot entry P26196 (GeneID 1656).
Specificity ABIN152327 is specific for human DDX6 protein. Species Reactivity: Human.
Cross-Reactivity Human
Purification Aff - Purified
Alternative Name DEAD box protein 6
Background DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), areputative RNA helicases. They are implicated in a number of cellular processes involvingalteration of RNA secondary structure such as translation initiation, nuclear andmitochondrial splicing, and ribosome and spliceosome assembly. Based on theirdistribution patterns, some members of this family are believed to be involved inembryogenesis, spermatogenesis, and cellular growth and division. This gene encodesa DEAD box protein. It may contribute to the cell proliferation and carcinogenesis. Alternate Names: anti-DDX6 antibody, anti-DDX-6 antibody, anti-DEAD (Asp-Glu-Ala-Asp) boxpolypeptide 6 antibody, anti-HLR2 antibody, anti-HLR-2 Helicase- RNA-Nuclear 2antibody, anti-P54 RNA Helicase antibody, anti-RCK antibody, anti-Breakpoint in RC-K8B-cell lymphoma cell line antibody.
Gene ID 1656
UniProt P26196
Application Notes Optimal working dilutions should be determined experimentally by the investigator.Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:5,000-1:15,000, Immunoprecipitation: 2-5 ug/ml Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Restrictions For Research Use only
Concentration 0.2 mg/ml
Buffer Tris-buffered Saline containing 0.1% BSA. Preservative: 0.09% Sodium Azide.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 6 (DDX6) antibody Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody ABIN152327 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody ABIN152328, but not with DDX6 Ab, which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).
General Nonhoff, Ralser, Welzel et al.: "Ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6 and interferes with P-bodies and stress granules." in: Molecular biology of the cell, Vol. 18, Issue 4, pp. 1385-96, 2007 (PubMed).

Hosts (24), (5), (3)
Reactivities (29), (10), (9), (3), (1), (1), (1)
Applications (31), (11), (7), (5), (2), (1), (1), (1)
Epitopes (8), (4), (2), (2), (1), (1), (1), (1), (1)
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