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Estrogen Receptor 2 (ER Beta) (ESR2) (AA 1-153) antibody

Details for Product No. ABIN152350, Supplier: Login to see
Antigen
  • ER[b]1
  • zfER-beta1
  • zfER[b]1
  • erb
  • esrb
  • estrb
  • nr3a2
  • ERbeta
  • er-beta
  • esr-beta
  • ESR2
  • er
  • esr2
  • erb2
  • erb1
  • ER-beta
  • ER[b]
  • Estrb
  • ER-BETA
  • ESR-BETA
  • ESRB
  • ESTRB
  • Erb
  • NR3A2
  • Erb2
  • ERB
  • ERBETA
  • CERB
  • ERBeta2
  • erbeta
  • Esr2
Alternatives
anti-Human Estrogen Receptor 2 (ER Beta) antibody for Immunofluorescence
Epitope
AA 1-153
67
33
27
22
18
18
17
15
14
14
13
13
11
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
330
97
81
9
9
8
8
4
2
2
1
1
Host
Mouse
265
69
15
5
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
19
18
15
11
9
9
3
3
3
3
3
3
3
3
3
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
255
132
50
42
40
34
25
18
12
11
8
4
3
3
3
3
1
Supplier
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Immunogen Amino acids 1-153 of human ER-Beta expressed in E. coli.
Clone 14C8
Isotype IgG2b
Specificity Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.
Cross-Reactivity (Details) Mouse reported in literature (PMID: 15750629)
Purification Protein G purified
Alternative Name Estrogen receptor beta (ESR2 Antibody Abstract)
Background The human ER-beta is a newly discovered estrogen receptor initially cloned andcharacterized from testis. The size and structure of ER-beta is very similar to ER-alphawith the ligand and DNA binding domains being highly conserved, while the aminoterminus which serves as their transactivation domains have diverged significantly.Similar in function to ER-alpha, ER-beta binds to estrogen with a high affinity andregulates estrogen specific gene activation through direct interaction with estrogenresponse elements (ERE',s). Alternate Names: anti-ER beta antibody.
Gene Symbol: ESR2
Gene ID 2100
Pathways Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway
Application Notes Strong ER beta staining in epithelial cell nuclei. Occassional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also detected ER beta in colon and ovarian tumors. Immunoprecipitation was reported in scientific literature.
Recommended dilutions: Chromatin Immunoprecipitation 1:10-1:500, Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 5 µg/mL, Immunoprecipitation 1:10-1:500, Western Blot 1-5 µg/mL
Protocol Immunohistochemistry protocol specific for Estrogen Receptor beta Antibody Immunohistochemistry Procedure
1. Cut 4-5 micron sections and mount onto Superfrost plus slides.
. Dewax in xylene and rehydrate through graded alcohols.
. Block with 3 % H2O2 in methanol for 10 minutes.
. Wash in running tap water for 5 minutes then block non-specific binding sites with normal horse serum.
. To unmask the antigen, heat-mediated retrieval is required using either a microwave or pressure cooker. For microwave-mediated retrieval immerse sections in 1 mM citrate buffer, pH 6, and microwaved at full power (600W) for 27 minutes . Alternatively, sections can be pressure cooked for 4 minutes, immersed in 1 mM citrate buffer, pH
. Allow sections to cool for 20-30 minutes and wash in running tap water for 5 minutes.
. Incubation sections with 5ug/ml primary antibody at 4C overnight. Incubation time and temperature are critical.
. Incubate with appropriate biotinylated secondary antibody for 30 minutes at room temperature, followed by streptavidin ABC kit (DAKO) according to the manufacturer's instructions.
. Prepare 3,3'-diaminobenzidine substrate and apply for 5-10 minutes.
. Counterstain with haematoxylin,dehydrate and coverslip.Using this protocol, we detect consistent, strong ERb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumours using the same antibody.
Restrictions For Research Use only
Buffer PBS, pH 7.4
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Immunohistochemistry (IHC) image for anti-Estrogen Receptor 2 (ER Beta) (ESR2) (AA 1-153) antibody (ABIN152350) Endocervical epithelium
Immunohistochemistry (IHC) image for anti-Estrogen Receptor 2 (ER Beta) (ESR2) (AA 1-153) antibody (ABIN152350) Infiltrating lobular carcinoma of the breast.
Background publications Leygue, Dotzlaw, Watson et al.: "Altered expression of estrogen receptor-alpha variant messenger RNAs between adjacent normal breast and breast tumor tissues." in: Breast cancer research : BCR, Vol. 2, Issue 1, pp. 64-72, 2001 (PubMed).

Skliris, Carder, Lansdown et al.: "Immunohistochemical detection of ERbeta in breast cancer: towards more detailed receptor profiling?" in: British journal of cancer, Vol. 84, Issue 8, pp. 1095-8, 2001 (PubMed).

Kuiper, Carlsson, Grandien et al.: "Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta." in: Endocrinology, Vol. 138, Issue 3, pp. 863-70, 1997 (PubMed).