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Estrogen Receptor 2 (ER Beta) (ESR2) antibody

Details for Product No. ABIN152350, Supplier: Log in to see
Antigen
  • ER[b]1
  • zfER-beta1
  • zfER[b]1
  • erb
  • esrb
  • estrb
  • nr3a2
  • ERbeta
  • er-beta
  • esr-beta
  • ESR2
  • er
  • esr2
  • erb2
  • erb1
  • ER-beta
  • ER[b]
  • Estrb
  • ER-BETA
  • ESR-BETA
  • ESRB
  • ESTRB
  • Erb
  • NR3A2
  • Erb2
  • ERB
  • ERBETA
  • CERB
  • ERBeta2
  • erbeta
  • Esr2
  • estrogen receptor 2b
  • estrogen receptor 2 (ER beta)
  • estrogen receptor beta
  • estrogen receptor beta 2
  • estrogen receptor beta 1
  • estrogen receptor type beta
  • estrogen receptor 2 (beta)
  • esr2b
  • esr2
  • ESR2
  • erb2
  • LOC100136443
  • erb1
  • LOC100304489
  • Esr2
  • erbeta
Alternatives
anti-Human Estrogen Receptor 2 (ER Beta) antibody for Immunofluorescence
Reactivity
Human, Monkey, Mouse (Murine)
407
163
136
39
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32
26
8
7
7
4
1
1
1
1
Host
Mouse
268
134
18
5
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
21
19
17
16
9
9
7
7
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
Application
Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
318
150
99
82
77
70
45
40
34
20
8
7
4
4
4
3
2
2
Options
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Immunogen Amino acids 1-153 of human ER-beta expressed in E. coli.
Clone 14C8
Isotype IgG2b
Specificity Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.
Purification Protein G purified
Alternative Name ER beta/nr3a2 (ESR2 Antibody Abstract)
Background Gene Symbol: ESR2
Molecular Weight Theoretical MW: 59 kDa
Gene ID 2100
UniProt Q92731
Pathways Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
Application Notes Western Blot 1-5 μg/mL, Chromatin Immunoprecipitation, Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation, Immunohistochemistry-Paraffin 1:10 - 1:500, Dot BlotIP usage reported in scientific literature. Strong ER beta staining in epithelial cell nuclei. Occassional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also detected ER beta in colon and ovarian tumors. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry protocol specific for Estrogen Receptor beta Antibody Immunohistochemistry Procedure
1. Cut 4-5 micron sections and mount onto Superfrost plus slides.
. Dewax in xylene and rehydrate through graded alcohols.
. Block with 3 % H2O2 in methanol for 10 minutes.
. Wash in running tap water for 5 minutes then block non-specific binding sites with normal horse serum.
. To unmask the antigen, heat-mediated retrieval is required using either a microwave or pressure cooker. For microwave-mediated retrieval immerse sections in 1 mM citrate buffer, pH 6, and microwaved at full power (600W) for 27 minutes . Alternatively, sections can be pressure cooked for 4 minutes, immersed in 1 mM citrate buffer, pH
. Allow sections to cool for 20-30 minutes and wash in running tap water for 5 minutes.
. Incubation sections with 5ug/ml primary antibody at 4C overnight. Incubation time and temperature are critical.
. Incubate with appropriate biotinylated secondary antibody for 30 minutes at room temperature, followed by streptavidin ABC kit (DAKO) according to the manufacturer's instructions.
. Prepare 3,3'-diaminobenzidine substrate and apply for 5-10 minutes.
. Counterstain with haematoxylin,dehydrate and coverslip.Using this protocol, we detect consistent, strong ERb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumours using the same antibody.
Restrictions For Research Use only
Format Liquid
Concentration 1.23 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Estrogen Receptor 2 (ER Beta) (ESR2) antibody (ABIN152350) Endocervical epithelium
Immunohistochemistry (IHC) image for anti-Estrogen Receptor 2 (ER Beta) (ESR2) antibody (ABIN152350) Infiltrating lobular carcinoma of the breast.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Estrogen Receptor 2 (ER Beta) (ESR2) antibody (ABIN152350) Immunohistochemistry-Paraffin: Estrogen Receptor beta Antibody (14C8) [ABIN152350] - ...
Western Blotting (WB) image for anti-Estrogen Receptor 2 (ER Beta) (ESR2) antibody (ABIN152350) Western Blot: ER beta/NR3A2 Antibody (14C8) [ABIN152350] - Non-transfected (-) and tr...
Product cited in: Kauffman, Robinson, Downes, Marcinkiewicz, Vourganti, Scherr, Gudas, Mongan: "Estrogen receptor-β expression and pharmacological targeting in bladder cancer." in: Oncology reports, Vol. 30, Issue 1, pp. 131-8, 2013 (PubMed).

López-García, E Cuevas, Corona-Quintanilla, Jiménez-Estrada, Martínez-Gómez, Castelán: "Effect of multiparity on morphometry and oestrogen receptor expression of pelvic and perineal striated muscles in rabbits: is serum oestradiol relevant?" in: European journal of obstetrics, gynecology, and reproductive biology, Vol. 169, Issue 1, pp. 113-20, 2013 (PubMed). (Sample species: Rabbit). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Jardel, Debiais, Godet, Irani, Fromont: "Ductal carcinoma of the prostate shows a different immunophenotype from high grade acinar cancer." in: Histopathology, Vol. 63, Issue 1, pp. 57-63, 2013 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Castelán, Xelhuantzi, Hernández-Aragón, Rodríguez-Antolín, Cuevas, Martínez-Gómez: "Morphometry of paravaginal ganglia from the pelvic plexus: impact of multiparity, primiparity, and pregnancy." in: European journal of obstetrics, gynecology, and reproductive biology, Vol. 170, Issue 1, pp. 286-92, 2013 (PubMed). (Sample species: Rabbit). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Honma, Saji, Hirose, Horiguchi, Kuroi, Hayashi, Utsumi, Harada: "Sex steroid hormones in pairs of tumor and serum from breast cancer patients and pathobiological role of androstene-3β, 17β-diol." in: Cancer science, Vol. 102, Issue 10, pp. 1848-54, 2012 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Navaratnam, Skliris, Qing, Banerji, Badiani, Tu, Bradbury, Leighl, Shepherd, Nowatzki, Demers, Murphy: "Differential role of estrogen receptor beta in early versus metastatic non-small cell lung cancer." in: Hormones & cancer, Vol. 3, Issue 3, pp. 93-100, 2012 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Verma, Miki, Abe, Niikawa, Sasano: "Cytoplasmic estrogen receptor β as a potential marker in human non-small cell lung carcinoma." in: Expert opinion on therapeutic targets, Vol. 16 Suppl 1, pp. S91-102, 2012 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Zuguchi, Miki, Onodera, Fujishima, Takeyama, Okamoto, Miyata, Sato, Satomi, Sasano: "Estrogen receptor α and β in esophageal squamous cell carcinoma." in: Cancer science, Vol. 103, Issue 7, pp. 1348-55, 2012 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Tonetti, Gao, Escarzaga, Walters, Szafran, Coon: "PKCα and ERβ Are Associated with Triple-Negative Breast Cancers in African American and Caucasian Patients." in: International journal of breast cancer, Vol. 2012, pp. 740353, 2012 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Karlsson, Helenius, Fernandes, Karlsson: "Oestrogen receptor β in NSCLC - prevalence, proliferative influence, prognostic impact and smoking." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 120, Issue 6, pp. 451-8, 2012 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Cheng, Li, Han, Lin, Niu, Zhou, Yuan, Huang, Li, Jiang, Zhang, Ding, Xu, Ye: "PES1 promotes breast cancer by differentially regulating ERα and ERβ." in: The Journal of clinical investigation, Vol. 122, Issue 8, pp. 2857-70, 2012 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Chromatin Immunoprecipitation

Sato, Suzuki, Katayose, Miura, Shiiba, Miki, Kamogawa, Yamamoto, Takayuki, Egawa, Unno, Sasano: "Aromatase in colon carcinoma." in: Anticancer research, Vol. 32, Issue 8, pp. 3069-75, 2012 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Verma, Miki, Abe, Nagasaki, Niikawa, Suzuki, Kondo, Sasano: "Co-expression of estrogen receptor beta and aromatase in Japanese lung cancer patients: gender-dependent clinical outcome." in: Life sciences, Vol. 91, Issue 15-16, pp. 800-8, 2012 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Sase, Suzuki, Miura, Shiiba, Sato, Nakamura, Takagi, Onodera, Miki, Watanabe, Ishida, Ohnuma, Sasaki, Sato, Karasawa, Shibata, Unno, Sasaki, Sasano: "Runt-related transcription factor 2 in human colon carcinoma: a potent prognostic factor associated with estrogen receptor." in: International journal of cancer. Journal international du cancer, Vol. 131, Issue 10, pp. 2284-93, 2012 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Paruthiyil, Cvoro, Tagliaferri, Cohen, Shtivelman, Leitman: "Estrogen receptor β causes a G2 cell cycle arrest by inhibiting CDK1 activity through the regulation of cyclin B1, GADD45A, and BTG2." in: Breast cancer research and treatment, Vol. 129, Issue 3, pp. 777-84, 2011 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Miki, Abe, Suzuki, Suzuki, Sasano: "Suppression of estrogen actions in human lung cancer." in: Molecular and cellular endocrinology, Vol. 340, Issue 2, pp. 168-74, 2011 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Vouk, Smuc, Guggenberger, Ribič-Pucelj, Sinkovec, Husen, Thole, Houba, Thaete, Adamski, Rižner: "Novel estrogen-related genes and potential biomarkers of ovarian endometriosis identified by differential expression analysis." in: The Journal of steroid biochemistry and molecular biology, Vol. 125, Issue 3-5, pp. 231-42, 2011 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Mehasseb, Panchal, Taylor, Brown, Bell, Habiba: "Estrogen and progesterone receptor isoform distribution through the menstrual cycle in uteri with and without adenomyosis." in: Fertility and sterility, Vol. 95, Issue 7, pp. 2228-35, 2235.e1, 2011 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Choijamts, Jimi, Kondo, Naganuma, Matsumoto, Kuroki, Iwasaki, Emoto: "CD133+ cancer stem cell-like cells derived from uterine carcinosarcoma (malignant mixed Müllerian tumor)." in: Stem cells (Dayton, Ohio), Vol. 29, Issue 10, pp. 1485-95, 2011 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Chakravarty, Gupta, Goda, Srinivasan, Patel, Dhaliwal: "Steroid receptors, HER2/neu and Ki-67, in endometrioid type of endometrial carcinoma: Correlation with conventional histomorphological features of prognosis." in: Acta histochemica, Vol. 112, Issue 4, pp. 355-63, 2010 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation