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Synapsin I (SYN1) antibody

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Synonyms Synapsin-1, SYN1a, SYN1b, SYNI, Syn-1, si:dkey-90n12.3, SYN I
Human, Mouse (Murine), Rat (Rattus)
(243), (194), (175), (44), (11), (10), (6), (4), (4), (2), (2), (1)
(283), (13)
(8), (8), (8), (6), (5), (5), (5), (5), (5), (5), (5), (5)
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(225), (72), (57), (49), (43), (26), (18), (12), (9), (3), (3), (1), (1)
Pubmed 7 references available
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Quantity 10 μg
Shipping to United States ( )
Immunogen Native protein purified from bovine brain
Specificity Specific for the ~78k synapsin I doublet in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.
Purification affinity purified
Alternative Name Synapsin-1 (SYN1 Antibody Abstract)
Background Synapsin I (synapsin Ia and Ib, MW ~ 80,000 and 77,000 are collectively referred to assynapsin I) is a neuron specific protein that is localized to nerve terminals. Thesynapsin protein is an excellent marker for synaptic terminals and it can be used toestimate synaptic density and or synaptogenesis. It is thought that synapsin I cross-links synaptic vesicles to the cytoskeleton and therebylimits the ability of these synaptic vesicles to move to active zones and fuse with theplasma membrane during exocytosis. In addition to their role in neurotransmission, the synapsins are also thought to play arole in synapse formation. The appearance of synapsin I immunoreactivity correlatesprecisely with the development of synapses in the CNS. The synapsin protein is anexcellent marker for synaptic terminals and it can be used to estimate synaptic densityand or synaptogenesis. The appearance of synapsin I was a precise indicator ofsynapse formation and that synapsin I immunocytochemistry provides a valuable tool forthe study of synaptogenesis. (2-4). Alternate Names: anti-SYNI antibody, anti-SYN I antibody, anti-SYN1 antibody, anti-SYN1a antibody,anti-SYN 1a antibody, anti-SYN1b antibody, anti-SYN 1b antibody, anti-SynapsinIantibody, anti-Synapsin1 antibody, anti-Synapsin 1 antibody, anti-Brain protein 4.1antibody.
Gene Symbol: SYN1
Gene ID 6853
Application Notes NB 300-104 can be used in Western blot where a doublet is seen at ~ 80 kDa representing synapsin Ia and Ib. Immunofluoresence, Immunoprecipitation (1 µg will quantitatively immunoprecipitate all synapsin in 200 µg of rat brain.) May work in IHC-Paraffin but this has not been tested. Differential distribution of the synapsins in the rat olfactory bulb. Has been reported in the literature to work in WB (rat cortex), IHC (cryosections) on rat olfacotry bulb. (PMID: 8283238)
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, Immunoprecipitation 1 µg per 200µg lysate, Western Blot 1:1000
Protocol Protocol specific for Synapsin I Antibody Protocol for Immunoprecipitation Reactions:
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 50 µL PBS (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
Buffer Lyophilized from 5 mM ammonium bicarbonate.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Synapsin I (SYN1) antibody (ABIN152453) Detection of Synapsin1 in rat hippocampal lysate using ABIN152453
Product cited in: Yang, Yan, Wood: "Kaposi's sarcoma-associated herpesvirus transactivator RTA promotes degradation of the repressors to regulate viral lytic replication." in: Journal of virology, Vol. 82, Issue 7, pp. 3590-603, 2008 (PubMed).

Kennedy, Martin, Ehrhardt et al.: "Requirement of JIP scaffold proteins for NMDA-mediated signal transduction." in: Genes & development, Vol. 21, Issue 18, pp. 2336-46, 2007 (PubMed).

Hanus, Ehrensperger, Triller: "Activity-dependent movements of postsynaptic scaffolds at inhibitory synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 17, pp. 4586-95, 2006 (PubMed).

Danglot, Triller, Bessis: "Association of gephyrin with synaptic and extrasynaptic GABAA receptors varies during development in cultured hippocampal neurons." in: Molecular and cellular neurosciences, Vol. 23, Issue 2, pp. 264-78, 2003 (PubMed).

Background publications Stone, Browning, Finger: "Differential distribution of the synapsins in the rat olfactory bulb." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 14, Issue 1, pp. 301-9, 1994 (PubMed).

Moore, Bernstein: "Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron microscopy and synapsin I immunoreactivity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 9, Issue 6, pp. 2151-62, 1989 (PubMed).

Fletcher, Cameron, De Camilli et al.: "The distribution of synapsin I and synaptophysin in hippocampal neurons developing in culture." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 11, Issue 6, pp. 1617-26, 1991 (PubMed).

Catalog No. ABIN152453
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