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Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (C-Term) antibody

Details for Product No. ABIN152454
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Antigen
Synonyms GluN2A, NMDAR2A, NR2A, EPND, GRIN2A, nr2a
Epitope
C-Term
(40), (38), (19), (18), (13), (7), (7), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(88), (88), (52), (46), (32), (31), (30), (3), (1)
Host
Rabbit
(107), (41), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (7), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(105), (66), (38), (33), (23), (17), (12), (8), (3), (2), (1)
Pubmed 5 references available
Quantity 0.01 mg
Shipping to United States (Change)
Catalog No. ABIN152454
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  • +1 404 474 4654
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Immunogen Fusion protein from the C-terminal region of the NR2A subunit
Specificity Specific for the ~180k NR2A subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2A subunit of NMDAR. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
Purification Affinity purified
Alternative Name NMDA Receptor 2A
Background The ion channels activated by glutamate are divided into two classes. Those that aresensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR)while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionicacid (AMPA) are known as kainate/AMPA receptors (K/AMPAR).NMDA receptors are among the most studied receptors in neuroscience because theyare involved in neuronal cell development and plasticity, a cellular correlate for learning.NMDA receptors are also implicated in several disorders of the central nervous systemincluding epilepsy and ischemic neuronal cell death. NMDA receptors also appear to bea target for ethanol at physiological concentrations and therefore may play a significantrole in alcoholism. Alternate Names: anti-Glutamate receptor antibody, anti-GRIN2A antibody, anti-HNR2A antibody, anti-NMethyl D Aspartate Receptor Subtype 2A antibody, anti-NMDA Receptor Type 2Aantibody, anti-NR2A antibody, anti-GRIN 2A antibody, anti-GRIN2A antibody,anti-Glutamate [NMDA] receptor subunit epsilon 1 antibody, anti-Glutamate receptorantibody, anti-Glutamate receptor ionotropic N methyl D aspartate 2A antibody,anti-HNR2A antibody, anti-N Methyl D Aspartate Receptor Subtype 2A antibody, anti-Nmethyl D aspartate receptor subunit 2A antibody, anti-NMDA Receptor Type 2Aantibody, anti-NMDA receptor subtype 2A antibody, anti-NMDAR 2A antibody, anti-NR2A antibody, anti-NR2A antibody, anti-NMDAR2A antibody,.
Gene Symbol: GRIN2A
Gene ID 2903, 24409
Application Notes NB 300-105 can be used in Western blot where a band is seen at ~ 180 kDa representing the NMDAR2A. Immunohistochemical analysis can be performed using frozen or fixed tissue sections. NB 300-105 can also be used to quantitatively immunoprecipitate the NMDAR2A where 3 ul will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2A in 200 mg of rat brain.
Recommended dilutions: Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:1000, Immunoprecipitation 3 µL per 200 µg lysate, Western Blot 1:1000
Protocol Western Blot Protocol specific for NMDAR2A Antibody Western Blot:**For brain tissue homogenize in hot 1 % SDS then run 7.5 % (as 2A is ~ 180 kDa) standard SDS gels and blot.
1. Thoroughly sonicate cell lysates or tissue homogenates to be loaded onto gel then dilute in appropriate sample buffer and boil for 5 minutes at 100C. Let samples cool to room temperature then load onto gel.
. Run SDS-PAGE per gel apparatus manufacturer'??s instructions.
. Transfer proteins to nitrocellulose or PVDF membrane (if using PVDF, be sure to activate membrane in Methanol prior to use).
. After transfer, air-dry blot to more stably fix proteins onto membrane.
. Block non-specific sites on membrane in 5 % NFDM (Non-fat dry milk) or 3 % BSA-TTBS (Tris-
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 50 µL phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4).
Buffer Lyophilized from 5 mM ammonium bicarbonate.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (C-Term) antibody anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (C-Term) antibody
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (C-Term) antibody (2) anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (C-Term) antibody (Image 2)
General Monyer, Sprengel, Schoepfer et al.: "Heteromeric NMDA receptors: molecular and functional distinction of subtypes." in: Science (New York, N.Y.), Vol. 256, Issue 5060, pp. 1217-21, 1992 (PubMed).

Moriyoshi, Masu, Ishii et al.: "Molecular cloning and characterization of the rat NMDA receptor." in: Nature, Vol. 354, Issue 6348, pp. 31-7, 1991 (PubMed).

Monyer, Burnashev, Laurie et al.: "Developmental and regional expression in the rat brain and functional properties of four NMDA receptors." in: Neuron, Vol. 12, Issue 3, pp. 529-40, 1994 (PubMed).

Laurie, Seeburg: "Ligand affinities at recombinant N-methyl-D-aspartate receptors depend on subunit composition." in: European journal of pharmacology, Vol. 268, Issue 3, pp. 335-45, 1995 (PubMed).

Ning, Pei, Liao et al.: "Dual neuroprotective signaling mediated by downregulating two distinct phosphatase activities of PTEN." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 16, pp. 4052-60, 2004 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN152454
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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