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NMDAR2A antibody (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a) (C-Term)

Details for Product anti-GRIN2A Antibody No. ABIN152454, Supplier: Log in to see
Antigen
  • NMDAR2A
  • nr2a
  • GRIN2A
  • EPND
  • GluN2A
  • NR2A
Epitope
C-Term
76
69
34
30
15
15
14
12
11
8
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
136
115
85
3
3
3
1
1
Host
Rabbit
135
75
4
Clonality
Polyclonal
Conjugate
This NMDAR2A antibody is un-conjugated
13
13
11
8
8
8
4
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
173
130
57
38
25
22
20
16
10
3
1
1
1
Supplier
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Immunogen Fusion protein from the C-terminal region of the NR2A subunit
Specificity Specific for the ~180k NR2A subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2A subunit of NMDAR. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
Purification Affinity purified
Alternative Name NMDA Receptor 2A (GRIN2A Antibody Abstract)
Background The ion channels activated by glutamate are divided into two classes. Those that aresensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR)while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionicacid (AMPA) are known as kainate/AMPA receptors (K/AMPAR).NMDA receptors are among the most studied receptors in neuroscience because theyare involved in neuronal cell development and plasticity, a cellular correlate for learning.NMDA receptors are also implicated in several disorders of the central nervous systemincluding epilepsy and ischemic neuronal cell death. NMDA receptors also appear to bea target for ethanol at physiological concentrations and therefore may play a significantrole in alcoholism. Alternate Names: anti-Glutamate receptor antibody, anti-GRIN2A antibody, anti-HNR2A antibody, anti-NMethyl D Aspartate Receptor Subtype 2A antibody, anti-NMDA Receptor Type 2Aantibody, anti-NR2A antibody, anti-GRIN 2A antibody, anti-GRIN2A antibody,anti-Glutamate [NMDA] receptor subunit epsilon 1 antibody, anti-Glutamate receptorantibody, anti-Glutamate receptor ionotropic N methyl D aspartate 2A antibody,anti-HNR2A antibody, anti-N Methyl D Aspartate Receptor Subtype 2A antibody, anti-Nmethyl D aspartate receptor subunit 2A antibody, anti-NMDA Receptor Type 2Aantibody, anti-NMDA receptor subtype 2A antibody, anti-NMDAR 2A antibody, anti-NR2A antibody, anti-NR2A antibody, anti-NMDAR2A antibody,.
Gene Symbol: GRIN2A
Gene ID 2903, 24409
Pathways
Application Notes NB 300-105 can be used in Western blot where a band is seen at ~ 180 kDa representing the NMDAR2A. Immunohistochemical analysis can be performed using frozen or fixed tissue sections. NB 300-105 can also be used to quantitatively immunoprecipitate the NMDAR2A where 3 ul will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2A in 200 mg of rat brain.
Recommended dilutions: Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:1000, Immunoprecipitation 3 µL per 200 µg lysate, Western Blot 1:1000
Protocol Western Blot Protocol specific for NMDAR2A Antibody Western Blot:**For brain tissue homogenize in hot 1 % SDS then run 7.5 % (as 2A is ~ 180 kDa) standard SDS gels and blot.
1. Thoroughly sonicate cell lysates or tissue homogenates to be loaded onto gel then dilute in appropriate sample buffer and boil for 5 minutes at 100C. Let samples cool to room temperature then load onto gel.
. Run SDS-PAGE per gel apparatus manufacturer'??s instructions.
. Transfer proteins to nitrocellulose or PVDF membrane (if using PVDF, be sure to activate membrane in Methanol prior to use).
. After transfer, air-dry blot to more stably fix proteins onto membrane.
. Block non-specific sites on membrane in 5 % NFDM (Non-fat dry milk) or 3 % BSA-TTBS (Tris-
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 50 µL phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4).
Buffer Lyophilized from 5 mM ammonium bicarbonate.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
Western Blotting (WB) image for anti-NMDAR2A antibody (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a) (C-Term) (ABIN152454) anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (C-Term) antibody
Western Blotting (WB) image for anti-NMDAR2A antibody (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a) (C-Term) (ABIN152454) anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A) (C-Term) antibody (Image 2)
Product cited in: Ning, Pei, Liao et al.: "Dual neuroprotective signaling mediated by downregulating two distinct phosphatase activities of PTEN." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 16, pp. 4052-60, 2004 (PubMed).

Background publications Laurie, Seeburg: "Ligand affinities at recombinant N-methyl-D-aspartate receptors depend on subunit composition." in: European journal of pharmacology, Vol. 268, Issue 3, pp. 335-45, 1995 (PubMed).

Monyer, Burnashev, Laurie et al.: "Developmental and regional expression in the rat brain and functional properties of four NMDA receptors." in: Neuron, Vol. 12, Issue 3, pp. 529-40, 1994 (PubMed).

Moriyoshi, Masu, Ishii et al.: "Molecular cloning and characterization of the rat NMDA receptor." in: Nature, Vol. 354, Issue 6348, pp. 31-7, 1991 (PubMed).

Monyer, Sprengel, Schoepfer et al.: "Heteromeric NMDA receptors: molecular and functional distinction of subtypes." in: Science (New York, N.Y.), Vol. 256, Issue 5060, pp. 1217-21, 1992 (PubMed).