Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (C-Term) antibody

Details for Product No. ABIN152455
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Antigen
Synonyms NMDAR2B, GluN2B, MRD6, NR2B, hNR3, nmdar2b, nr2b, AW490526, Nmdar2b
Epitope
C-Term
(50), (43), (41), (38), (36), (27), (22), (22), (17), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(314), (293), (257), (138), (136), (135), (70), (17), (8), (7), (4), (3), (3), (2), (2)
Host
Rabbit
(302), (57), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(11), (11), (11), (9), (9), (9), (9), (9), (9), (9), (9), (6), (6), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(231), (94), (86), (79), (71), (70), (52), (38), (18), (11), (4), (1)
Pubmed 8 references available
Quantity 0.01 mg
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Catalog No. ABIN152455
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Immunogen Fusion protein from the C-terminus of the NR2B subunit of rat NMDA receptor.
Specificity Specific for the ~180kDa NR2B subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2B subunits of NMDAR. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits.
Purification Affinity purified
Alternative Name NMDAR2B
Background The ion channels activated by glutamate are divided into two classes. Those that aresensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR)while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionicacid (AMPA) are known as kainate/AMPA receptors (K/AMPAR).NMDA receptors are among the most studied receptors in neuroscience because theyare involved in neuronal cell development and plasticity, a cellular correlate for learning.NMDA receptors are also implicated in several disorders of the central nervous systemincluding epilepsy and ischemic neuronal cell death. NMDA receptors also appear to bea target for ethanol at physiological concentrations and therefore may play a significantrole in alcoholism. Alternate Names: anti-NMDAR2B antibody, anti-Glutamate Receptor Ionotropic N Methyl D Aspartate 2Bantibody, anti-Glutamate [NMDA] receptor subunit epsilon 2 antibody, anti-Glutamatereceptor ionotropic NMDA2B antibody, anti-MGC142178 antibody, anti-MGC142180antibody, anti-N METHYL D ASPARTATE RECEPTOR CHANNEL SUBUNIT EPSILON2 antibody, anti-N methyl D aspartate receptor subtype 2B antibody, anti-N methyl Daspartate receptor subunit 2B antibody, anti-N methyl D aspartate receptor subunit 3antibody, anti-NMDA NR2B antibody, anti-NMDA R2B antibody, anti-NR2B antibody,anti-NR3 antibody, anti-Nmdar2b antibody, anti-hNR 3 antibody, anti-hNR3 antibody,anti-GRIN 2B antibody, anti-GRIN2B antibody, anti-hNR 3 antibody.
Gene Symbol: GRIN2B
Gene ID 2904, 14812, 24410
Research Area Neurology, Neurotransmitters, Receptors
Application Notes NB300-106 can be used in Western blot where a band is seen at ~ 180 kDa representing the NMDAR2B. Immunohistochemical analysis can be performed using frozen or fixed tissue sections. NB300-106 can also be used to quantitatively immunoprecipitate NMDAR2B where 3 ul will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200 mg of rat brain. NB300-106 has also been used successfully in immunofluoresence as reported in the literature (PMID: 20082293) and reported by an anonymous customer review. After reconstitution the antibody should be aliquoted and stored at -20C. Adequate amount of material to conduct 10-mini Western Blots.
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Frozen 1:1000-1:2000, Immunoprecipitation 3 µL per 200 µg lysate, Western Blot 1:1000
Protocol Protocol specific for NMDA receptor 2B Antibody Western Blot ProtocolThoroughly sonicate tissue in the following buffer:1 % SDS (hot, but not boiling, ~ 90C )10 mM Tris pH 8.01 mM EDTA
1. Dilute in appropriate SDS PAGE sample buffer and boil for 5 minutes at 100 C. Let samples cool to room temperature then load onto gel.
. Run SDS-PAGE per gel apparatus manufacturer?s instructions.
. Transfer proteins to nitrocellulose or PVDF membrane (if using PVDF, be sure to activate membrane in Methanol prior to use).
. After transfer, air-dry blot (~15 min.) to more stably fix proteins onto membrane.
. Block non-specific sites on membrane in 5 % NFDM (Non-fat dry milk) -TTBS(Tris-
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute with 50 µL of PBS.
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (C-Term) antibody NR2B in rat hippocampus stained with ABIN152455.
anti-Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) (C-Term) antibody (2) Lane 1:20 ug of rat brain hippocampus was loaded and ABIN152455 labels a band at 180 kDa. Lanes 2-3: HEK 293 cells expressing NR2A or NR2B respectively. The antibody labels only NR2B and shows no reactivity with NR2A. Non-specific staining of additional bands is seen in HEK293 cells but not in rat brain.
General Monyer, Sprengel, Schoepfer et al.: "Heteromeric NMDA receptors: molecular and functional distinction of subtypes." in: Science (New York, N.Y.), Vol. 256, Issue 5060, pp. 1217-21, 1992 (PubMed).

Moriyoshi, Masu, Ishii et al.: "Molecular cloning and characterization of the rat NMDA receptor." in: Nature, Vol. 354, Issue 6348, pp. 31-7, 1991 (PubMed).

Monyer, Burnashev, Laurie et al.: "Developmental and regional expression in the rat brain and functional properties of four NMDA receptors." in: Neuron, Vol. 12, Issue 3, pp. 529-40, 1994 (PubMed).

Laurie, Seeburg: "Ligand affinities at recombinant N-methyl-D-aspartate receptors depend on subunit composition." in: European journal of pharmacology, Vol. 268, Issue 3, pp. 335-45, 1995 (PubMed).

Gore: "Gonadotropin-releasing hormone neurons, NMDA receptors, and their regulation by steroid hormones across the reproductive life cycle." in: Brain research. Brain research reviews, Vol. 37, Issue 1-3, pp. 235-48, 2001 (PubMed).

Ning, Pei, Liao et al.: "Dual neuroprotective signaling mediated by downregulating two distinct phosphatase activities of PTEN." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 16, pp. 4052-60, 2004 (PubMed).

Gabra, Kessler, Ritter et al.: "Decrease in N-methyl-D-aspartic acid receptor-NR2B subunit levels by intrathecal short-hairpin RNA blocks group I metabotropic glutamate receptor-mediated hyperalgesia." in: The Journal of pharmacology and experimental therapeutics, Vol. 322, Issue 1, pp. 186-94, 2007 (PubMed).

Bai, Zeng, Hu et al.: "Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants." in: Biotechnology letters, Vol. 29, Issue 12, pp. 2007-12, 2007 (PubMed).

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