tyrosine Hydroxylase (TH) antibody

Antigen: tyrosine Hydroxylase (TH)

Synonyms: TYH, DYT14, DYT5b, The, th

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN152458

Additional Information

Alternative name: Tyrosine 3-monooxygenase (TH)

Immunogen: Raised aginst SDS denatured rat tyrosine hydroxylase purified frompheochromocytoma.

Description: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of thecatecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation ofthe TH enzyme represents the central means for controlling the synthesis of theseimportant catecholamines. TH has a large molecular diversity, resulting from differentialsplicing of its mRNA, which is tissue specific and might result in long term changes inactivity of the enzyme and its availability of neurotransmitter at various synapses. The presence of different DNA sequences at the TH locus confers susceptibility tovarious disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are aconsistent neurochemical abnormality. Alternate Names: anti-TH antibody, anti-Tyrosine hydroxylase antibody, anti-Tyrosine 3-monooxygenaseantibody, anti-L-tyrosine hydroxylase antibody, anti-Tyrosine 3-hydroxylase antibody,anti-Neuronal Marker antibody,.

Specificity: ABIN152458 is specific for tyrosine hydroxylase. Species Reactivity: Detects all mammalian forms of TH tested to date and some non-mammalian. Alsodetects some invertebrate forms of TH. Localization: ABIN152458 can be used as a marker for dopaminergic and noradrenergic neurons.

Application Details

Application Notes: Positive Controls: PC-12 cells stimulated by okadaic acid

Buffer: 10 HEPES [pH7.5], 150 mM NaCl, & 100ug/ml BSA. Preservative: 50% glycerol.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Amino Acids, Enzymes

Restrictions: For Research Use only

Images

Immunohistochemical staining of retina tissue during ABIN152458.

Publications

Renfroe, Chronister, Haycock et al.: "The localization of tyrosine hydroxylase-like immunoreactivity in the central nervous system: methodological considerations." in: Brain research bulletin, Vol. 13, Issue 1, pp. 109-26, 1984 (PubMed).

Haycock: "Polyvinylpyrrolidone as a blocking agent in immunochemical studies." in: Analytical biochemistry, Vol. 208, Issue 2, pp. 397-9, 1993 (PubMed).

Kumer, Vrana: "Intricate regulation of tyrosine hydroxylase activity and gene expression." in: Journal of neurochemistry, Vol. 67, Issue 2, pp. 443-62, 1996 (PubMed).

Guo, Lai, Longhurst: "Medullary pathways involved in cardiac sympathoexcitatory reflexes in the cat." in: Brain research, Vol. 925, Issue 1, pp. 55-66, 2001 (PubMed).

Nocjar, Roth, Pehek: "Localization of 5-HT(2A) receptors on dopamine cells in subnuclei of the midbrain A10 cell group." in: Neuroscience, Vol. 111, Issue 1, pp. 163-76, 2002 (PubMed).

Guo, Li, Longhurst: "Central pathways in the pons and midbrain involved in cardiac sympathoexcitatory reflexes in cats." in: Neuroscience, Vol. 113, Issue 2, pp. 435-47, 2002 (PubMed).

Selvage, Lee, Parsons et al.: "A hypothalamic-testicular neural pathway is influenced by brain catecholamines, but not testicular blood flow." in: Endocrinology, Vol. 145, Issue 4, pp. 1750-9, 2004 (PubMed).

Von Coelln, Thomas, Savitt et al.: "Loss of locus coeruleus neurons and reduced startle in parkin null mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 29, pp. 10744-9, 2004 (PubMed).

Kunz, Scott, Ittner et al.: "Calcitonin gene-related peptide-evoked sustained tachycardia in calcitonin receptor-like receptor transgenic mice is mediated by sympathetic activity." in: American journal of physiology. Heart and circulatory physiology, Vol. 293, Issue 4, pp. H2155-60, 2007 (PubMed).