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Tachykinin Receptor 1 (TACR1) (AA 1-50), (N-Term) antibody

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N-Term, AA 1-50
(27), (16), (13), (12), (12), (6), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Guinea Pig, Human, Mouse (Murine), Rat (Rattus)
(121), (41), (35), (5), (2), (2), (2), (2), (1), (1), (1)
(110), (15), (7)
(7), (7), (6), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(80), (48), (34), (17), (11), (10), (8), (5), (4), (2)
Pubmed 16 references available
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Quantity 0.1 mL
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Immunogen A peptide derived from the N-terminus region between amino acids 1-50 of human Neurokinin 1 Receptor Immunogen has 94% sequence identity with guinea pig. Mouse reactivity is from a customer review
Purification affinity purified
Background The tachykinins belong to an evolutionary conserved family of peptide neurotransmittersthat share the c-terminal sequence Phe-X-Gly-Leu-Met-NH2 and have an establishedrole in neurotransmission. The mammalian tachykinins include substance P, neurokininA (NKA) and neurokinin B (NKB) which exert their effects by binding to specificreceptors. Tachykinin peptides are important in the mediation of many physiologicaland pathological processes including inflammation, pain, migraine headache and allergyinduced asthma. Three tachykinin receptor types have been characterized, NK-1, NK-2 and NK-3 whichhave preferential affinities for SP, NKA and NKB respectively. All three receptors sharea high degree of sequence homology, have seven transmembrane spanning domainsand similar signal transduction mechanisms (e.g. G-protein coupled activation ofphospholipase C). Alternate Names: anti-NK1 receptor antibody, anti-NK 1 receptor antibody, anti-NK-1 receptor antibody,anti-NK1R antibody, anti-NK 1R antibody, anti-SPR antibody, anti-Substance P receptorantibody, anti-TAC1R antibody, anti-TAC 1R antibody, anti-Tachykinin receptor 1antibody, anti-tachykinin 1 receptor, anti-TACR1 antibody, anti-NK-1r antibody,anti-neurokinin 1 receptor antibody.
Gene Symbol: TACR1
Gene ID 6869, 24807
UniProt P25103
Research Area Hormones, Receptors
Application Notes This Neurokinin 1 Receptor Antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western blot.
Recommended dilutions: Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry 10 µg/mL, Immunohistochemistry-Paraffin 10 µg/mL, Western Blot 1-2 µg/mL
Protocol Protocol specific for Neurokinin 1 Receptor Antibody Western Analysis1) Separate 50 µg of whole cell or membrane protein on a 4-20 % Tris-glycine gel.
2) Transfer the proteins from the gel onto a nitrocellulose membrane.
) Incubate the nitrocellulose membrane in Blocking solution [5 % non-fat dry milk / PBS] overnight at 4 degrees C.
) Incubate the membrane with a 1:1,000 dilution of anti-NK-1 antibody (Novus Biologicals, Inc, #NB300-119) in PBST-NFDM [PBS / 0.1 % Tween-20 / 5 % non-fat dry milk] for 2 hours at RT.
) Wash the membrane with PBST-NFDM.
) Incubate the membrane with HRP-conjugated goat anti-rabbit IgG diluted with PBST-NFDM for 1 hour at RT.
) Detect antibody with an enhanced chemiluminescence reaction (ECL Western Blotting detection kit, Pharmacia).FACS Analysis
1. Seed cells into small (T-25) flask. Add 5-10 mL media. Allow cells to grow to 80-100 % confluence.
. When flasks are confluent, empty media and add new media containing appropriate drug, hormone, or cytokine dose.
. Allow flasks to incubate for 24 hours. After incubation period, remove 2 mL of media and place 1 mL into one of two eppendorf tubes.
. Empty off remaining media. Wash with 5 mL 1X PBS.
. Add 2 mL PBS with azide (1 % Goat serum).
. Scrape cells using a cell scraper.
. Transfer cells to appropriate labeled FACSCAN tubes. Vortex all tubes
. Incubate on ice for - hour in the PBS with azide (1 % Goat serum). Vortex all tubes after incubation.
. Centrifuge at 1400 RPM for 5 minutes. Empty off media.
. Resuspend in 1 mL of a 1:3,000 primary antibody solution [1 µL antibody per 3 mL PBS with azide (1 % Goat serum)].
. Incubate on ice for 1 hour. Vortex all tubes after incubation.
. Centrifuge at 1400 RPM for 5 minutes. Aspirate (or decant) supernatant.
. Wash twice with 2 mL ice-cold PBS with azide (1 % Goat serum).
. Aspirate (or decant) supernatant and place tubes on ice.
. Resuspend in 1 mL of a 1:500 secondary antibody solution [2 µL antibody per 1 mL PBS with azide (1 % Goat serum)].
. Vortex all tubes.
. Cover with foil. Incubate for 30 minutes on ice.
. Vortex after incubation. Centrifuge at 1400 RPM for 5 minutes.
. Aspirate (or decant) supernatant. Wash twice with 2 mL ice-cold PBS with azide (1 % Goat serum).
. Aspirate (or decant) supernatant. Resuspend in 2 % paraformaldehyde.
. Read on flow.FACS Analysis procedure courtesy of: Rosalyn Blumenthal, Ph.D. Director Tumor Biology Garden State Cancer Center Belleville, NJ 07109IHC-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, 30 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-Tachykinin Receptor 1 (TACR1) (AA 1-50), (N-Term) antibody (ABIN152468) Staining of brain, basal nucleus of meynert, neurons using ABIN152468.
Product cited in: Nayate, Moore, Weiss et al.: "Cardiac damage after lesions of the nucleus tractus solitarii." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 296, Issue 2, pp. R272-9, 2009 (PubMed).

Schmidtko, Gao, Koenig et al.: "cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase I." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 34, pp. 8568-76, 2008 (PubMed).

Hazlett, McClellan, Barrett et al.: "Spantide I decreases type I cytokines, enhances IL-10, and reduces corneal perforation in susceptible mice after Pseudomonas aeruginosa infection." in: Investigative ophthalmology & visual science, Vol. 48, Issue 2, pp. 797-807, 2007 (PubMed).

Borghese, Werner, Topf et al.: "An isoflurane- and alcohol-insensitive mutant GABA(A) receptor alpha(1) subunit with near-normal apparent affinity for GABA: characterization in heterologous systems and production of knockin mice." in: The Journal of pharmacology and experimental therapeutics, Vol. 319, Issue 1, pp. 208-18, 2006 (PubMed).

Lai, Ho, Kilpatrick et al.: "Full-length and truncated neurokinin-1 receptor expression and function during monocyte/macrophage differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 20, pp. 7771-6, 2006 (PubMed).

Tooney, Au, Chahl: "Localisation of tachykinin NK1 and NK3 receptors in the human prefrontal and visual cortex." in: Neuroscience letters, Vol. 283, Issue 3, pp. 185-8, 2000 (PubMed).

Background publications Fong, Potts: "Neurokinin-1 receptor activation in Botzinger complex evokes bradypnoea." in: The Journal of physiology, Vol. 575, Issue Pt 3, pp. 869-85, 2006 (PubMed).

Benarroch, Schmeichel, Low et al.: "Depletion of ventromedullary NK-1 receptor-immunoreactive neurons in multiple system atrophy." in: Brain : a journal of neurology, Vol. 126, Issue Pt 10, pp. 2183-90, 2003 (PubMed).

Tooney, Crawter, Chahl: "Increased tachykinin NK(1) receptor immunoreactivity in the prefrontal cortex in schizophrenia." in: Biological psychiatry, Vol. 49, Issue 6, pp. 523-7, 2001 (PubMed).

Mantyh, Rogers, Honore et al.: "Inhibition of hyperalgesia by ablation of lamina I spinal neurons expressing the substance P receptor." in: Science (New York, N.Y.), Vol. 278, Issue 5336, pp. 275-9, 1997 (PubMed).

Allen, Rogers, Ghilardi et al.: "Noxious cutaneous thermal stimuli induce a graded release of endogenous substance P in the spinal cord: imaging peptide action in vivo." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 17, Issue 15, pp. 5921-7, 1997 (PubMed).

Mantyh, Pappas, Lapp et al.: "Substance P activation of enteric neurons in response to intraluminal Clostridium difficile toxin A in the rat ileum." in: Gastroenterology, Vol. 111, Issue 5, pp. 1272-80, 1996 (PubMed).

Mantyh, Rogers, Ghilardi et al.: "Differential expression of two isoforms of the neurokinin-1 (substance P) receptor in vivo." in: Brain research, Vol. 719, Issue 1-2, pp. 8-13, 1997 (PubMed).

Mantyh, DeMaster, Malhotra et al.: "Receptor endocytosis and dendrite reshaping in spinal neurons after somatosensory stimulation." in: Science (New York, N.Y.), Vol. 268, Issue 5217, pp. 1629-32, 1995 (PubMed).

Mantyh, Allen, Ghilardi et al.: "Rapid endocytosis of a G protein-coupled receptor: substance P evoked internalization of its receptor in the rat striatum in vivo." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 7, pp. 2622-6, 1995 (PubMed).

Vigna, Bowden, McDonald et al.: "Characterization of antibodies to the rat substance P (NK-1) receptor and to a chimeric substance P receptor expressed in mammalian cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 14, Issue 2, pp. 834-45, 1994 (PubMed).

Catalog No. ABIN152468

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