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TACR1 antibody (Tachykinin Receptor 1) (N-Term)

Details for Product anti-TACR1 Antibody No. ABIN152468, Supplier: Log in to see
Antigen
  • ASPR
  • nk1R
  • NK1R
  • Nk1r
  • NKIR
  • Spr
  • SPR
  • Tac1r
  • TAC1R
  • Tacr1
Epitope
N-Term
26
18
17
14
12
12
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
136
58
49
7
5
3
2
2
1
1
1
1
1
Host
Rabbit
125
15
8
Clonality
Polyclonal
Conjugate
This TACR1 antibody is un-conjugated
8
7
7
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
90
48
45
23
20
19
12
12
7
2
1
Supplier
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Immunogen A peptide derived from the N-terminus region between amino acids 1-50 of human Neurokinin 1 Receptor [UniProt# P25103]
Purification Immunogen affinity purified
Alternative Name NK1R (TACR1 Antibody Abstract)
Background Gene Symbol: TACR1
Molecular Weight Theoretical MW: 46 kDa
Gene ID 6869, 24807
UniProt P25103
Application Notes Western Blot 1-2 μg/mL, Flow Cytometry 1:10-1:1000, Immunohistochemistry 10 μg/mL, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 10 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Neurokinin 1 Receptor Antibody Western Analysis1) Separate 50 µg of whole cell or membrane protein on a 4-20 % Tris-glycine gel.
2) Transfer the proteins from the gel onto a nitrocellulose membrane.
) Incubate the nitrocellulose membrane in Blocking solution [5 % non-fat dry milk / PBS] overnight at 4 degrees C.
) Incubate the membrane with a 1:1,000 dilution of anti-NK-1 antibody (Novus Biologicals, Inc, #NB300-119) in PBST-NFDM [PBS / 0.1 % Tween-20 / 5 % non-fat dry milk] for 2 hours at RT.
) Wash the membrane with PBST-NFDM.
) Incubate the membrane with HRP-conjugated goat anti-rabbit IgG diluted with PBST-NFDM for 1 hour at RT.
) Detect antibody with an enhanced chemiluminescence reaction (ECL Western Blotting detection kit, Pharmacia).FACS Analysis
1. Seed cells into small (T-25) flask. Add 5-10 mL media. Allow cells to grow to 80-100 % confluence.
. When flasks are confluent, empty media and add new media containing appropriate drug, hormone, or cytokine dose.
. Allow flasks to incubate for 24 hours. After incubation period, remove 2 mL of media and place 1 mL into one of two eppendorf tubes.
. Empty off remaining media. Wash with 5 mL 1X PBS.
. Add 2 mL PBS with azide (1 % Goat serum).
. Scrape cells using a cell scraper.
. Transfer cells to appropriate labeled FACSCAN tubes. Vortex all tubes
. Incubate on ice for - hour in the PBS with azide (1 % Goat serum). Vortex all tubes after incubation.
. Centrifuge at 1400 RPM for 5 minutes. Empty off media.
. Resuspend in 1 mL of a 1:3,000 primary antibody solution [1 µL antibody per 3 mL PBS with azide (1 % Goat serum)].
. Incubate on ice for 1 hour. Vortex all tubes after incubation.
. Centrifuge at 1400 RPM for 5 minutes. Aspirate (or decant) supernatant.
. Wash twice with 2 mL ice-cold PBS with azide (1 % Goat serum).
. Aspirate (or decant) supernatant and place tubes on ice.
. Resuspend in 1 mL of a 1:500 secondary antibody solution [2 µL antibody per 1 mL PBS with azide (1 % Goat serum)].
. Vortex all tubes.
. Cover with foil. Incubate for 30 minutes on ice.
. Vortex after incubation. Centrifuge at 1400 RPM for 5 minutes.
. Aspirate (or decant) supernatant. Wash twice with 2 mL ice-cold PBS with azide (1 % Goat serum).
. Aspirate (or decant) supernatant. Resuspend in 2 % paraformaldehyde.
. Read on flow.FACS Analysis procedure courtesy of: Rosalyn Blumenthal, Ph.D. Director Tumor Biology Garden State Cancer Center Belleville, NJ 07109IHC-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-TACR1 antibody (Tachykinin Receptor 1) (N-Term) (ABIN152468) Staining of brain, basal nucleus of meynert, neurons using ABIN152468.
Western Blotting (WB) image for anti-TACR1 antibody (Tachykinin Receptor 1) (N-Term) (ABIN152468) Western Blot: Neurokinin 1 Receptor Antibody [ABIN152468] - Analysis of Neurokinin 1 ...
Western Blotting (WB) image for anti-TACR1 antibody (Tachykinin Receptor 1) (N-Term) (ABIN152468) Western Blot: NK1R Antibody [ABIN152468] - Total protein from human, mouse and rat br...
Immunofluorescence (IF) image for anti-TACR1 antibody (Tachykinin Receptor 1) (N-Term) (ABIN152468) Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [ABIN152468] -...
Product cited in: Zhou, Zuo, Wang, Zhang, Yu, Yang, Yao: "Effect of truncated neurokinin-1 receptor expression changes on the interaction between human breast cancer and bone marrow-derived mesenchymal stem cells." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 19, Issue 9, pp. 676-91, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Fujisawa, Kashiwakura, Kita, Kikukawa, Fujitani, Sasaki-Sakamoto, Kuroda, Nunomura, Hayama, Terui, Ra, Okayama: "Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria." in: The Journal of allergy and clinical immunology, Vol. 134, Issue 3, pp. 622-633.e9, 2014 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Schwartz, Spitsin, Meshki, Tuluc, Douglas, Wolfe: "Substance P enhances HIV-1 infection in human fetal brain cell cultures expressing full-length neurokinin-1 receptor." in: Journal of neurovirology, Vol. 19, Issue 3, pp. 219-27, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Zhou, Zhao, Xiong, Chen, Zhang, Yu, Yang, Yao: "Roles of full-length and truncated neurokinin-1 receptors on tumor progression and distant metastasis in human breast cancer." in: Breast cancer research and treatment, Vol. 140, Issue 1, pp. 49-61, 2013 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Altawil, Lyström, El-Nour: "Kinetics of neuronal contribution during the development of a contact allergic reaction." in: Archives of dermatological research, Vol. 304, Issue 4, pp. 273-81, 2012 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Humes, Simpson, Smith, Sutton, Zaitoun, Bush, Bennett, Scholefield, Spiller: "Visceral hypersensitivity in symptomatic diverticular disease and the role of neuropeptides and low grade inflammation." in: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, Vol. 24, Issue 4, pp. 318-e163, 2012 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Janssen, Yasuda, Vicini: "GABA(A) Receptor β3 Subunit Expression Regulates Tonic Current in Developing Striatopallidal Medium Spiny Neurons." in: Frontiers in cellular neuroscience, Vol. 5, pp. 15, 2011 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Nayate, Moore, Weiss, Taktakishvili, Lin, Talman: "Cardiac damage after lesions of the nucleus tractus solitarii." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 296, Issue 2, pp. R272-9, 2009 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

Schmidtko, Gao, König, Heine, Motterlini, Ruth, Schlossmann, Koesling, Niederberger, Tegeder, Friebe, Geisslinger: "cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase I." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 34, pp. 8568-76, 2008 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Grimsholm, Rantapää-Dahlqvist, Dalén, Forsgren et al.: "Observations favouring the occurrence of local production and marked effects of bombesin/gastrin-releasing peptide in the synovial tissue of the human knee joint--comparisons with substance P and the ..." in: Neuropeptides, Vol. 42, Issue 2, pp. 133-45, 2008 (PubMed). (Sample species: Human).

Andersson, Danielson, Alfredson, Forsgren: "Presence of substance P and the neurokinin-1 receptor in tenocytes of the human Achilles tendon." in: Regulatory peptides, Vol. 150, Issue 1-3, pp. 81-7, 2008 (PubMed). (Sample species: Human).

Massouh, Wallman, Pourcher, Parent: "The fate of the large striatal interneurons expressing calretinin in Huntington's disease." in: Neuroscience research, Vol. 62, Issue 4, pp. 216-24, 2008 (PubMed). (Sample species: Human).

Hazlett, McClellan, Barrett, Liu, Zhang, Lighvani: "Spantide I decreases type I cytokines, enhances IL-10, and reduces corneal perforation in susceptible mice after Pseudomonas aeruginosa infection." in: Investigative ophthalmology & visual science, Vol. 48, Issue 2, pp. 797-807, 2007 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Andersson, Danielson, Alfredson, Forsgren: "Nerve-related characteristics of ventral paratendinous tissue in chronic Achilles tendinosis." in: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, Vol. 15, Issue 10, pp. 1272-9, 2007 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)

Ferguson, Hardy, Werner, Hileman, Delorey, Homanics: "New insight into the role of the beta3 subunit of the GABAA-R in development, behavior, body weight regulation, and anesthesia revealed by conditional gene knockout." in: BMC neuroscience, Vol. 8, pp. 85, 2007 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Lévesque, Wallman, Parent, Sík, Parent: "Neurokinin-1 and neurokinin-3 receptors in primate substantia nigra." in: Neuroscience research, Vol. 57, Issue 3, pp. 362-71, 2007 (PubMed).

Lai, Ho, Kilpatrick, Wang, Tuluc, Korchak, Douglas: "Full-length and truncated neurokinin-1 receptor expression and function during monocyte/macrophage differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 20, pp. 7771-6, 2006 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Borghese, Werner, Topf, Baron, Henderson, Boehm, Blednov, Saad, Dai, Pearce, Harris, Homanics, Harrison: "An isoflurane- and alcohol-insensitive mutant GABA(A) receptor alpha(1) subunit with near-normal apparent affinity for GABA: characterization in heterologous systems and production of knockin mice." in: The Journal of pharmacology and experimental therapeutics, Vol. 319, Issue 1, pp. 208-18, 2006 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Fong, Potts: "Neurokinin-1 receptor activation in Botzinger complex evokes bradypnoea." in: The Journal of physiology, Vol. 575, Issue Pt 3, pp. 869-85, 2006 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

Lévesque, Parent, Parent: "Cellular and subcellular localization of neurokinin-1 and neurokinin-3 receptors in primate globus pallidus." in: The European journal of neuroscience, Vol. 23, Issue 10, pp. 2760-72, 2006 (PubMed).