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TACR1 antibody (Tachykinin Receptor 1) (N-Term)

Details for Product anti-TACR1 Antibody No. ABIN152468, Supplier: Log in to see
Antigen
  • NK1R
  • NKIR
  • SPR
  • TAC1R
  • Nk1r
  • Spr
  • Tac1r
  • nk1R
  • Tacr1
  • ASPR
  • substance-P receptor-like
  • tachykinin receptor 1
  • LOC100053491
  • TACR1
  • Tacr1
Alternatives
anti-Human TACR1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
N-Term
22
18
17
15
12
11
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
141
71
62
13
10
6
6
5
5
4
2
2
1
1
1
1
1
Host
Rabbit
129
16
8
Clonality
Polyclonal
Conjugate
This TACR1 antibody is un-conjugated
8
8
7
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
98
48
47
30
24
19
16
13
7
2
1
1
Options
Supplier
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Immunogen A peptide derived from the N-terminus region between amino acids 1-50 of human Neurokinin 1 Receptor [UniProt# P25103]
Purification Immunogen affinity purified
Alternative Name NK1R (TACR1 Antibody Abstract)
Background Gene Symbol: TACR1
Molecular Weight Theoretical MW: 46 kDa
Gene ID 6869
UniProt P25103
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones
Application Notes Western Blot 1-2 μg/mL, Flow Cytometry 1:10-1:1000, Immunohistochemistry 10 μg/mL, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 10 μg/mLThis Neurokinin 1 Receptor Antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Western blot. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Neurokinin 1 Receptor Antibody Western Analysis1) Separate 50 µg of whole cell or membrane protein on a 4-20 % Tris-glycine gel.
2) Transfer the proteins from the gel onto a nitrocellulose membrane.
) Incubate the nitrocellulose membrane in Blocking solution [5 % non-fat dry milk / PBS] overnight at 4 degrees C.
) Incubate the membrane with a 1:1,000 dilution of anti-NK-1 antibody (Novus Biologicals, Inc, #NB300-119) in PBST-NFDM [PBS / 0.1 % Tween-20 / 5 % non-fat dry milk] for 2 hours at RT.
) Wash the membrane with PBST-NFDM.
) Incubate the membrane with HRP-conjugated goat anti-rabbit IgG diluted with PBST-NFDM for 1 hour at RT.
) Detect antibody with an enhanced chemiluminescence reaction (ECL Western Blotting detection kit, Pharmacia).FACS Analysis
1. Seed cells into small (T-25) flask. Add 5-10 mL media. Allow cells to grow to 80-100 % confluence.
. When flasks are confluent, empty media and add new media containing appropriate drug, hormone, or cytokine dose.
. Allow flasks to incubate for 24 hours. After incubation period, remove 2 mL of media and place 1 mL into one of two eppendorf tubes.
. Empty off remaining media. Wash with 5 mL 1X PBS.
. Add 2 mL PBS with azide (1 % Goat serum).
. Scrape cells using a cell scraper.
. Transfer cells to appropriate labeled FACSCAN tubes. Vortex all tubes
. Incubate on ice for - hour in the PBS with azide (1 % Goat serum). Vortex all tubes after incubation.
. Centrifuge at 1400 RPM for 5 minutes. Empty off media.
. Resuspend in 1 mL of a 1:3,000 primary antibody solution [1 µL antibody per 3 mL PBS with azide (1 % Goat serum)].
. Incubate on ice for 1 hour. Vortex all tubes after incubation.
. Centrifuge at 1400 RPM for 5 minutes. Aspirate (or decant) supernatant.
. Wash twice with 2 mL ice-cold PBS with azide (1 % Goat serum).
. Aspirate (or decant) supernatant and place tubes on ice.
. Resuspend in 1 mL of a 1:500 secondary antibody solution [2 µL antibody per 1 mL PBS with azide (1 % Goat serum)].
. Vortex all tubes.
. Cover with foil. Incubate for 30 minutes on ice.
. Vortex after incubation. Centrifuge at 1400 RPM for 5 minutes.
. Aspirate (or decant) supernatant. Wash twice with 2 mL ice-cold PBS with azide (1 % Goat serum).
. Aspirate (or decant) supernatant. Resuspend in 2 % paraformaldehyde.
. Read on flow.FACS Analysis procedure courtesy of: Rosalyn Blumenthal, Ph.D. Director Tumor Biology Garden State Cancer Center Belleville, NJ 07109IHC-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-TACR1 antibody (Tachykinin Receptor 1) (N-Term) (ABIN152468) Staining of brain, basal nucleus of meynert, neurons using ABIN152468.
Western Blotting (WB) image for anti-TACR1 antibody (Tachykinin Receptor 1) (N-Term) (ABIN152468) Western Blot: Neurokinin 1 Receptor Antibody [ABIN152468] - Analysis of Neurokinin 1 ...
Western Blotting (WB) image for anti-TACR1 antibody (Tachykinin Receptor 1) (N-Term) (ABIN152468) Western Blot: NK1R Antibody [ABIN152468] - Total protein from human, mouse and rat br...
Immunofluorescence (IF) image for anti-TACR1 antibody (Tachykinin Receptor 1) (N-Term) (ABIN152468) Immunocytochemistry/Immunofluorescence: Neurokinin 1 Receptor Antibody [ABIN152468] -...
Product cited in: Zhou, Zuo, Wang, Zhang, Yu, Yang, Yao: "Effect of truncated neurokinin-1 receptor expression changes on the interaction between human breast cancer and bone marrow-derived mesenchymal stem cells." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 19, Issue 9, pp. 676-91, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Fujisawa, Kashiwakura, Kita, Kikukawa, Fujitani, Sasaki-Sakamoto, Kuroda, Nunomura, Hayama, Terui, Ra, Okayama: "Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria." in: The Journal of allergy and clinical immunology, Vol. 134, Issue 3, pp. 622-633.e9, 2014 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Schwartz, Spitsin, Meshki, Tuluc, Douglas, Wolfe: "Substance P enhances HIV-1 infection in human fetal brain cell cultures expressing full-length neurokinin-1 receptor." in: Journal of neurovirology, Vol. 19, Issue 3, pp. 219-27, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Zhou, Zhao, Xiong, Chen, Zhang, Yu, Yang, Yao: "Roles of full-length and truncated neurokinin-1 receptors on tumor progression and distant metastasis in human breast cancer." in: Breast cancer research and treatment, Vol. 140, Issue 1, pp. 49-61, 2013 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Altawil, Lyström, El-Nour: "Kinetics of neuronal contribution during the development of a contact allergic reaction." in: Archives of dermatological research, Vol. 304, Issue 4, pp. 273-81, 2012 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Humes, Simpson, Smith, Sutton, Zaitoun, Bush, Bennett, Scholefield, Spiller: "Visceral hypersensitivity in symptomatic diverticular disease and the role of neuropeptides and low grade inflammation." in: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, Vol. 24, Issue 4, pp. 318-e163, 2012 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Janssen, Yasuda, Vicini: "GABA(A) Receptor β3 Subunit Expression Regulates Tonic Current in Developing Striatopallidal Medium Spiny Neurons." in: Frontiers in cellular neuroscience, Vol. 5, pp. 15, 2011 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Nayate, Moore, Weiss, Taktakishvili, Lin, Talman: "Cardiac damage after lesions of the nucleus tractus solitarii." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 296, Issue 2, pp. R272-9, 2009 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

Schmidtko, Gao, König, Heine, Motterlini, Ruth, Schlossmann, Koesling, Niederberger, Tegeder, Friebe, Geisslinger: "cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase I." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 34, pp. 8568-76, 2008 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Grimsholm, Rantapää-Dahlqvist, Dalén, Forsgren et al.: "Observations favouring the occurrence of local production and marked effects of bombesin/gastrin-releasing peptide in the synovial tissue of the human knee joint--comparisons with substance P and the ..." in: Neuropeptides, Vol. 42, Issue 2, pp. 133-45, 2008 (PubMed). (Sample species: Human).

Andersson, Danielson, Alfredson, Forsgren: "Presence of substance P and the neurokinin-1 receptor in tenocytes of the human Achilles tendon." in: Regulatory peptides, Vol. 150, Issue 1-3, pp. 81-7, 2008 (PubMed). (Sample species: Human).

Massouh, Wallman, Pourcher, Parent: "The fate of the large striatal interneurons expressing calretinin in Huntington's disease." in: Neuroscience research, Vol. 62, Issue 4, pp. 216-24, 2008 (PubMed). (Sample species: Human).

Hazlett, McClellan, Barrett, Liu, Zhang, Lighvani: "Spantide I decreases type I cytokines, enhances IL-10, and reduces corneal perforation in susceptible mice after Pseudomonas aeruginosa infection." in: Investigative ophthalmology & visual science, Vol. 48, Issue 2, pp. 797-807, 2007 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Andersson, Danielson, Alfredson, Forsgren: "Nerve-related characteristics of ventral paratendinous tissue in chronic Achilles tendinosis." in: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, Vol. 15, Issue 10, pp. 1272-9, 2007 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)

Ferguson, Hardy, Werner, Hileman, Delorey, Homanics: "New insight into the role of the beta3 subunit of the GABAA-R in development, behavior, body weight regulation, and anesthesia revealed by conditional gene knockout." in: BMC neuroscience, Vol. 8, pp. 85, 2007 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Lévesque, Wallman, Parent, Sík, Parent: "Neurokinin-1 and neurokinin-3 receptors in primate substantia nigra." in: Neuroscience research, Vol. 57, Issue 3, pp. 362-71, 2007 (PubMed).

Lai, Ho, Kilpatrick, Wang, Tuluc, Korchak, Douglas: "Full-length and truncated neurokinin-1 receptor expression and function during monocyte/macrophage differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 20, pp. 7771-6, 2006 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Borghese, Werner, Topf, Baron, Henderson, Boehm, Blednov, Saad, Dai, Pearce, Harris, Homanics, Harrison: "An isoflurane- and alcohol-insensitive mutant GABA(A) receptor alpha(1) subunit with near-normal apparent affinity for GABA: characterization in heterologous systems and production of knockin mice." in: The Journal of pharmacology and experimental therapeutics, Vol. 319, Issue 1, pp. 208-18, 2006 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Fong, Potts: "Neurokinin-1 receptor activation in Botzinger complex evokes bradypnoea." in: The Journal of physiology, Vol. 575, Issue Pt 3, pp. 869-85, 2006 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

Lévesque, Parent, Parent: "Cellular and subcellular localization of neurokinin-1 and neurokinin-3 receptors in primate globus pallidus." in: The European journal of neuroscience, Vol. 23, Issue 10, pp. 2760-72, 2006 (PubMed).