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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Cow (Bovine), Caenorhabditis elegans (C. elegans), Chicken, Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Plant
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|14 references available|
|Quantity||50 µL (5 mg/ml)|
|Price||Product not available in this region.|
|Gene ID||6233, 22190|
|Immunogen||Purified ubiquitin conjugated to keyhole limpet hemocyanin with glutaraldehyde.|
|Description||Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has anormal role in the targeting of proteins for proteolytic degradation. To perform thisfunction, the protein to be degraded is first covalently attached to the C-terminus ofubiquitin, and the ubiquitinated complex is then recognized by a complex of degradativeenzymes. Interestingly, ubiquitin also becomes covalently bonded to many types ofpathological inclusions, which appear to be resistant to normal degradation. Therefore,ubiquitin antibodies are very useful for studies of these inclusions. For example, theneurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease,Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease areall heavily ubiquitinated and can be readily visualized with ubiquitin antibodies. Alternate Names: anti-60S ribosomal protein L40 antibody, anti-HUBCEP52 antibody, anti-RPL40antibody, anti-Ubiquitin 52 amino acid fusion protein antibody, anti-Ubiquitin A52 residueribosomal protein fusion product 1 antibody, anti-Ubiquitin carboxyl extension protein 52antibody, anti-Ubiquitin CEP52 antibody.|
|Specificity||ABIN152476 specifically recognizes ubiquitinated cytoplasmic inclusion bodies. Species Reactivity: ABIN152476 reacts with human, bovine, mouse, chicken, Drosophila, C. elegans, andArabidopsis thaliana (plant) ubiquitin and ubiquitinated proteins in ELISA reactions. Thisantibody recognizes polyubiquitin chains much more strongly than monoubiquitinatedmolecules or free ubiquitin.|
|Application Notes||Immunohistochemistry 1:1000,Western Blot 1:5000,Immunohistochemistry-Paraffin 1:1000,Immunohistochemistry-Frozen 1:1000|
|Buffer||Preservative: 5 mM sodium azide.|
|Storage||Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.|
|Research Area||Neurology, Proteolysis / Ubiquitin, Alzheimer's Disease, Proteases, Amino Acids|
|Restrictions||For Research Use only|
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|Reactivities||Caenorhabditis elegans (C. elegans) (1), Chicken (1), Cow (Bovine) (1), Fruit Fly (Drosophila melanogaster) (1), Human (1), Mouse (Murine) (1)|
|Applications||ELISA (1), Immunocytochemistry (ICC) (1), Immunofluorescence (IF) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1), Western Blotting (WB) (1)|