Ubiquitin antibody

Details for Product No. ABIN152476, Supplier: Log in to see
Antigen
  • CG11624
  • DmUb
  • DmUbi-p63E
  • Dmel\\CG11624
  • Ub
  • Ubi-P63e
  • Ubi-p
  • Ubi63E
  • Ubi63p
  • Ubip
  • Ubiq
  • Ubp
  • ubi
  • ubi-p63E
  • ubi63E
  • CG18282
  • CG32744
  • CR32744
  • DmUbi-p5E
  • Dmel\\CG32744
  • ubiquitin
  • si:dz180g5.5
  • si:busm1-180g5.5
  • LOC100194618
  • UBIQ
  • Ubiquitin
  • LOC100282068
  • polyubiquitin-B-like
  • Ubiquitin-63E
  • Ubiquitin-5E
  • transmembrane and ubiquitin-like domain containing 2
  • polyubiquitin
  • polyubiquitin containing 7 ubiquitin monomers
  • Ubiquitin
  • ubiquitin
  • ubiquitin B
  • LOC100716362
  • Ubi-p63E
  • Ubi-p5E
  • tmub2
  • LOC100194618
  • LOC100282068
  • LOC100190766
  • LOC100135798
  • UBB
Reactivity
Caenorhabditis elegans (C. elegans), Chicken, Cow (Bovine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Plant, Rat (Rattus)
323
195
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183
59
47
38
35
30
28
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26
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24
13
8
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Host
Mouse
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207
5
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
18
15
13
13
13
12
8
8
8
8
8
8
8
8
8
8
6
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
367
238
185
134
122
118
67
32
22
13
13
12
7
3
1
1
1
Options
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Immunogen Purified Ubiquitin conjugated to KLH with glutaraldehyde.
Clone Ubi-1
Isotype IgG1 kappa
Specificity Recognizes polyubiquitin chains much more strongly than monoubiquitinated molecules or free ubiquitin. Specifically recognizes ubiquitinated cytoplasmic inclusion bodies.
Purification Unpurified
Background Gene Symbol: RPS27A
Molecular Weight Theoretical MW: 8.5 kDa
Gene ID 6233
UniProt P0CG48, P0CG47
Research Area Neurology, Proteolysis / Ubiquitin, Alzheimer's Disease, Proteases, Amino Acids
Pathways Mitotic G1-G1/S Phases
Application Notes Western Blot 1:1000-1:5000, ELISA 1:100-1:2000, Immunohistochemistry 1:500-1:1000, Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry-Paraffin 1:500-1:1000, Immunohistochemistry-Frozen 1:500-1:1000This Ubiquitin Antibody (Ubi-1) is useful for Western blot, ELISA, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on both paraffin-embedded and frozen sections. In Western blot a band can be seen at 8.5 kDa. This antibody is excellent for the detection of ubiquitinated inclusions seen in human neurodegenerative diseases such as the neurofibrillary tangles of Alzheimer's disease. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry-Paraffin Protocol Specific for NB300-130: Ubiquitin Antibody (Ubi-1)Materials1) 1 Phosphate buffered saline (pH 7.6): NaCl 137mmol/L, KCl 2.7mmol/L, Na2HPO4 4.3mmol/L, KH2PO4 1.4 mmol/L2) Citrate buffer, 0.01 M, pH6.0, Sodium Citrate 3g, Citric acid 0.4g3) 3 % Hydrogen peroxide4) Primary antibody5) Blocking serum (normal serum)6) Biotinylated secondary antibody7) DAB staining kitMethods
1. Dewax and hydration of slides using xylene and EtOH:Dry slides for 20 min in a 60 C ovenAdd Xylene, 2 x 10 min100 %, 95 %, 80 %, and 70 % EtOH, 5 min each EtOH concentration Rinse in PBS, 5'2 Antigen retrieval method (only for paraffin slides)1a. High-pressure antigen retrieval procedure (recommended method)Place slides in a glass slide holder (ensure that the slide holder is completely filled with slides, slides without sections if necessary, to ensure even heating. The entire slide holder is immersed in 1000 mL of Citrate buffer (0.01M, pH6.
0) within a pressure cookerOnce steam is produced, and ONLY when steam is visible, from the pressure cooker (usually 15-20 min), the required high-pressure will have been reached, and slides will be incubated for 2 min.Turn off heat, and allow buffer and slides to cool to room temperatureSlides are then rinsed in PBS for 5 minutes
. Add 3 % hydrogen peroxide solution, 10'at RT, then PBS, 3X5'
. Normal blocking serum, 20'at RT
. Incubate with Primary Ab, 4C overnight or 1.5 hours at 37C
. Rinse with PBS, 3 X 5' each rinse
. Add Biotin-conjugated second antibody, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Add Streptavidin-Peroxidase, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Staining with DAB solution, 2-5'under microscope
. Stop the reaction by washing in tap water
. Counterstain in Haematoxylin for 3-5 minutes
. 75 %, 80 %, 95 % and 100 % ethanol, 5x2', xylene 2 x 10'
Restrictions For Research Use only
Format Liquid
Buffer Ascites
Buffer contains: 10 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Ubiquitin antibody (ABIN152476) Immunohistochemical staining of Ubiquitin in hippocampal tissue from an Alzheimer pat...
Western Blotting (WB) image for anti-Ubiquitin antibody (ABIN152476) Staining of ubiquitin using ABIN152476. Lane 1 is mono-ubiquitin and polyubiquitin c...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Ubiquitin antibody (ABIN152476) Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [ABIN152476] - IHC staining...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Ubiquitin antibody (ABIN152476) Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [ABIN152476] - IHC-P staini...
Product cited in: Pruneda, Durkin, Geurink, Ovaa, Santhanam, Holden, Komander: "The Molecular Basis for Ubiquitin and Ubiquitin-like Specificities in Bacterial Effector Proteases." in: Molecular cell, Vol. 63, Issue 2, pp. 261-76, 2016 (PubMed). Further details: Western Blotting

Damgaard, Walker, Marco-Casanova, Morgan, Titheradge, Elliott, McHale, Maher, McKenzie, Komander: "The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity." in: Cell, Vol. 166, Issue 5, pp. 1215-1230.e20, 2016 (PubMed). Further details: Western Blotting

Schwab, Ebert: "Neurite Aggregation and Calcium Dysfunction in iPSC-Derived Sensory Neurons with Parkinson's Disease-Related LRRK2 G2019S Mutation." in: Stem cell reports, Vol. 5, Issue 6, pp. 1039-52, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Begum, Yan, Li, Singh, Dixon, Sun: "Docosahexaenoic acid reduces ER stress and abnormal protein accumulation and improves neuronal function following traumatic brain injury." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 10, pp. 3743-55, 2014 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Frozen Sections)

Ejlerskov, Rasmussen, Nielsen, Bergström, Tohyama, Jensen, Vilhardt: "Tubulin polymerization-promoting protein (TPPP/p25α) promotes unconventional secretion of α-synuclein through exophagy by impairing autophagosome-lysosome fusion." in: The Journal of biological chemistry, Vol. 288, Issue 24, pp. 17313-35, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Rosenfeldt, Nixon, Liu, Mah, Ryan: "Analysis of macroautophagy by immunohistochemistry." in: Autophagy, Vol. 8, Issue 6, pp. 963-9, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Maroui, Kheddache-Atmane, El Asmi, Dianoux, Aubry, Chelbi-Alix: "Requirement of PML SUMO interacting motif for RNF4- or arsenic trioxide-induced degradation of nuclear PML isoforms." in: PLoS ONE, Vol. 7, Issue 9, pp. e44949, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Dayal, Sparks, Jacob, Allende-Vega, Lane, Saville: "Suppression of the deubiquitinating enzyme USP5 causes the accumulation of unanchored polyubiquitin and the activation of p53." in: The Journal of biological chemistry, Vol. 284, Issue 8, pp. 5030-41, 2009 (PubMed). (Sample species: Human). Further details: Western Blotting

Stuttmann, Lechner, Guérois, Parker, Nussaume, Genschik, Noël: "COP9 signalosome- and 26S proteasome-dependent regulation of SCFTIR1 accumulation in Arabidopsis." in: The Journal of biological chemistry, Vol. 284, Issue 12, pp. 7920-30, 2009 (PubMed). (Sample species: Plant). Further details: Western Blotting

Watson, Lagow, Xu, Zhang, Bonini: "A drosophila model for amyotrophic lateral sclerosis reveals motor neuron damage by human SOD1." in: The Journal of biological chemistry, Vol. 283, Issue 36, pp. 24972-81, 2008 (PubMed). (Sample species: Fruit Fly (Drosophila melanogaster)). Further details: Immunohistochemistry

Cripps, Thomas, Jeng, Yang, Davies, Yang: "Alzheimer disease-specific conformation of hyperphosphorylated paired helical filament-Tau is polyubiquitinated through Lys-48, Lys-11, and Lys-6 ubiquitin conjugation." in: The Journal of biological chemistry, Vol. 281, Issue 16, pp. 10825-38, 2006 (PubMed). (Sample species: Human). Further details: Western Blotting

Chang, Kamata, Solinas, Luo, Maeda, Venuprasad, Liu, Karin: "The E3 ubiquitin ligase itch couples JNK activation to TNFalpha-induced cell death by inducing c-FLIP(L) turnover." in: Cell, Vol. 124, Issue 3, pp. 601-13, 2006 (PubMed). Further details: Western Blotting

Lichtenfels, Kellner, Atkins, Bukur, Ackermann, Beck, Brenner, Melchior, Seliger: "Identification of metabolic enzymes in renal cell carcinoma utilizing PROTEOMEX analyses." in: Biochimica et biophysica acta, Vol. 1646, Issue 1-2, pp. 21-31, 2003 (PubMed). (Sample species: Human). Further details: Western Blotting

Background publications Laroia, Cuesta, Brewer, Schneider: "Control of mRNA decay by heat shock-ubiquitin-proteasome pathway." in: Science (New York, N.Y.), Vol. 284, Issue 5413, pp. 499-502, 1999 (PubMed).

Sternsdorf, Puccetti, Jensen, Hoelzer, Will, Ottmann, Ruthardt: "PIC-1/SUMO-1-modified PML-retinoic acid receptor alpha mediates arsenic trioxide-induced apoptosis in acute promyelocytic leukemia." in: Molecular and cellular biology, Vol. 19, Issue 7, pp. 5170-8, 1999 (PubMed).

Marti, Wirbelauer, Scheffner, Krek: "Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation." in: Nature cell biology, Vol. 1, Issue 1, pp. 14-9, 1999 (PubMed).

Sternsdorf, Jensen, Will: "Evidence for covalent modification of the nuclear dot-associated proteins PML and Sp100 by PIC1/SUMO-1." in: The Journal of cell biology, Vol. 139, Issue 7, pp. 1621-34, 1998 (PubMed).

Cuervo, Hu, Lim, Dice: "IkappaB is a substrate for a selective pathway of lysosomal proteolysis." in: Molecular biology of the cell, Vol. 9, Issue 8, pp. 1995-2010, 1998 (PubMed).

Verdier, Chruetien, Muller, Varlet, Yoshimura, Gisselbrecht, Lacombe, Mayeux: "Proteasomes regulate erythropoietin receptor and signal transducer and activator of transcription 5 (STAT5) activation. Possible involvement of the ubiquitinated Cis protein." in: The Journal of biological chemistry, Vol. 273, Issue 43, pp. 28185-90, 1998 (PubMed).

Hirano, Kimoto, Shimoyama, Hirohashi, Takeichi: "Identification of a neural alpha-catenin as a key regulator of cadherin function and multicellular organization." in: Cell, Vol. 70, Issue 2, pp. 293-301, 1992 (PubMed).