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ATP1A1 antibody (ATPase, Na+/K+ Transporting, alpha 1 Polypeptide)

Details for Product anti-ATP1A1 Antibody No. ABIN152492, Supplier: Login to see New
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Dog (Canine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine), Xenopus laevis
(93), (91), (74), (9), (7), (7), (7), (6), (1), (1), (1)
(110), (13), (1)
Clonality (Clone)
Monoclonal ()
This ATP1A1 antibody is un-conjugated
(5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(82), (37), (31), (20), (16), (15), (7), (6), (3), (2), (1)
Pubmed 16 references available
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Quantity 50 μL
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Immunogen Purified Sodium Potassium ATPase Alpha 1 from rabbit renal outer medulla.
Clone 464-6
Isotype IgG1 kappa
Specificity This is specific for Na,K-ATPase alpha 1 subunit.
Purification Ascites
Alternative Name ATP1A1 (ATP1A1 Antibody Abstract)
Background Sodium Potassium ATPase is an integral membrane protein complex that hydrolyzesATP to maintain the transmembrane gradients of Na+ and K+ found in most mammaliancells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptidehas been shown to be the catalytically active subunit, wherease the Beta-polypeptideappears to be necessary for the assembly and transport of the sodium pump to theplasma membrane.Sodium Potassium ATPase alpha-1 subunit, in brain, is expressed in both neuronal andnon-neuronal cells. Alternate Names: anti-ATP1A1 antibody, anti-Na+/K+ ATPase 1 antibody, anti-Sodium pump 1 antibody,anti-ATPase Na+/K+ transporting alpha 1 polypeptide antibody, anti-Na+/K+ ATPasealpha-1 subunit antibody, anti-Na+, K+ ATPase alpha subunit antibody, anti-alpha 1sodium potassium ATPase antibody, anti-sodium potassium ATPase alpha A catalyticpolypeptide antibody, anti-sodium pump subunit alpha-1 antibody, anti-MGC3285antibody, anti-MGC51750 antibody, anti-ATPase alpha 1 (Na/K) antibody.
Gene Symbol: ATP1A1
Gene ID 476, 25650
UniProt Q9N0Z6
Application Notes This Sodium Potassium ATPase (464.6) antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and Western Blot. By Western Blot, a distinct band at 112 kDa is seen. Do not boil the sample prior to loading on the gel for Western Blot (60 °C appears to work fine).
Recommended dilutions: Flow Cytometry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:1000, Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200, Western Blot 1:1000-1:10000
Protocol Protocol specific for Sodium Potassium ATPase Alpha 1 Antibody Procedure Guide for NB 300-146 Monoclonal anti-Na-K-ATPase Antibody (464.6)I. Western Blot Procedure
1. Run ~20 µg of Triton-treated porcine proximal tubule extract protein on a 7.5 % SDS-PAGE gel.*
. Transfer protein to the membrane using a Tris-Glycine/Methanol buffer.
. Block membrane with TBST/5 % NFDM for 30 min. at room temperature (~23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with TBST.
. Incubate membrane with 1:5,000 dilution of NB300-146 (anti-Na,K-ATPase), diluted in TBST, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with TBST.
. Incubate membrane with 1:15,000 dilution of goat anti-mouse IgG-HRP [(, diluted in TBST, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with TBST.
. Detect cross-reacting proteins using Chemiluminescent substrate according to manufacturers guidelines.* NOTE: Do not boil the protein samples, as boiling causes aggregation of the Na,K-ATPase. The aggregate band will appear at ~150 kDa on Western Blots.II. Quench Endogenous Peroxidase: IHC-FFPE sections
. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.
. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.- To Prepare 200 mL of Quenching Solution:- Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.- Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.
. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Western Blotting (WB) image for anti-ATP1A1 antibody (ATPase, Na+/K+ Transporting, alpha 1 Polypeptide) (ABIN152492) Western Blot analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule prote...
Immunohistochemistry (IHC) image for anti-ATP1A1 antibody (ATPase, Na+/K+ Transporting, alpha 1 Polypeptide) (ABIN152492) Staining of enodmetrial glands within the uterus using ABIN152492. Note the absence o...
Product cited in: Peyrottes, Navarro, Ondo-Mendez et al.: "Immunoanalysis indicates that the sodium iodide symporter is not overexpressed in intracellular compartments in thyroid and breast cancers." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 160, Issue 2, pp. 215-25, 2009 (PubMed).

Burgess, Parkinson, Racke et al.: "ABCG1 influences the brain cholesterol biosynthetic pathway but does not affect amyloid precursor protein or apolipoprotein E metabolism in vivo." in: Journal of lipid research, Vol. 49, Issue 6, pp. 1254-67, 2008 (PubMed).

Kadereit, Kumar, Wang et al.: "Evolutionarily conserved gene family important for fat storage." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 1, pp. 94-9, 2008 (PubMed).

Padilla, Cottrell, Roosterman et al.: "Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins." in: The Journal of cell biology, Vol. 179, Issue 5, pp. 981-97, 2007 (PubMed).

Jahn, Leifheit, Gooch et al.: "Lipid rafts are required for Kit survival and proliferation signals." in: Blood, Vol. 110, Issue 6, pp. 1739-47, 2007 (PubMed).

Qu, Perdomo, Su et al.: "Effects of apoA-V on HDL and VLDL metabolism in APOC3 transgenic mice." in: Journal of lipid research, Vol. 48, Issue 7, pp. 1476-87, 2007 (PubMed).

Tansley, Burgess, Bryan et al.: "The cholesterol transporter ABCG1 modulates the subcellular distribution and proteolytic processing of beta-amyloid precursor protein." in: Journal of lipid research, Vol. 48, Issue 5, pp. 1022-34, 2007 (PubMed).

Perry, Dembla-Rajpal, Hall et al.: "A three-dimensional model of human organic anion transporter 1: aromatic amino acids required for substrate transport." in: The Journal of biological chemistry, Vol. 281, Issue 49, pp. 38071-9, 2006 (PubMed).

Pessach, Shmelzer, Leto et al.: "The C-terminal flavin domain of gp91phox bound to plasma membranes of granulocyte-like X-CGD PLB-985 cells is sufficient to anchor cytosolic oxidase components and support NADPH oxidase-associated ..." in: Journal of leukocyte biology, Vol. 80, Issue 3, pp. 630-9, 2006 (PubMed).

Tarn, Skorobogatko, Taguchi et al.: "Therapeutic effect of imatinib in gastrointestinal stromal tumors: AKT signaling dependent and independent mechanisms." in: Cancer research, Vol. 66, Issue 10, pp. 5477-86, 2006 (PubMed).

Mukhopadhyay, Shah, Patel et al.: "Monocrotaline pyrrole-induced megalocytosis of lung and breast epithelial cells: Disruption of plasma membrane and Golgi dynamics and an enhanced unfolded protein response." in: Toxicology and applied pharmacology, Vol. 211, Issue 3, pp. 209-20, 2006 (PubMed).

Pohl, Ring, Korkmaz et al.: "FAT/CD36-mediated long-chain fatty acid uptake in adipocytes requires plasma membrane rafts." in: Molecular biology of the cell, Vol. 16, Issue 1, pp. 24-31, 2004 (PubMed).

Holzer, Katano, Klomp et al.: "Cisplatin rapidly down-regulates its own influx transporter hCTR1 in cultured human ovarian carcinoma cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 19, pp. 6744-9, 2004 (PubMed).

Knox, Belous, Pierce et al.: "Novel role of phospholipase C-delta1: regulation of liver mitochondrial Ca2+ uptake." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 287, Issue 3, pp. G533-40, 2004 (PubMed).

Xavier, Piek, Fujii et al.: "Amelioration of radiation-induced fibrosis: inhibition of transforming growth factor-beta signaling by halofuginone." in: The Journal of biological chemistry, Vol. 279, Issue 15, pp. 15167-76, 2004 (PubMed).

Background publications Winterstein, Trotter, Krämer-Albers: "Distinct endocytic recycling of myelin proteins promotes oligodendroglial membrane remodeling." in: Journal of cell science, Vol. 121, Issue Pt 6, pp. 834-42, 2008 (PubMed).

Catalog No. ABIN152492

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