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ATP1A1 antibody (ATPase, Na+/K+ Transporting, alpha 1 Polypeptide)

Details for Product anti-ATP1A1 Antibody No. ABIN152492, Supplier: Log in to see
Antigen
  • atp1a1
  • atp1a1a.1
  • Atpa-1
  • BC010319
  • K-ATPase
  • Na+K+ATPase
  • Nkaa1b
Reactivity
Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Primate, Rabbit, Rat (Rattus), Xenopus laevis, Yeast
91
91
74
9
7
7
7
6
2
1
1
Host
Mouse
105
14
1
Clonality (Clone)
Monoclonal ()
Conjugate
This ATP1A1 antibody is un-conjugated
4
3
3
3
3
3
3
3
3
3
3
3
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
80
36
30
21
16
15
7
7
2
1
1
Supplier
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Immunogen Purified Sodium Potassium ATPase Alpha 1 from rabbit renal outer medulla. [UniProt# Q9N0Z6]
Clone 464-6
Isotype IgG1 kappa
Specificity This is specific for Na,K-ATPase alpha 1 subunit.
Purification Ascites
Alternative Name ATP1A1 (ATP1A1 Antibody Abstract)
Background Gene Symbol: ATP1A1
Molecular Weight Theoretical MW: 112 kDa
UniProt P05023, Q9N0Z6
Application Notes Western Blot 1:1000-1:10000, Flow Cytometry 1:50-1:200, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:50-1:1000, Immunohistochemistry-Paraffin 1:200, Immunohistochemistry-Frozen
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Sodium Potassium ATPase Alpha 1 Antibody Procedure Guide for NB 300-146 Monoclonal anti-Na-K-ATPase Antibody (464.6)I. Western Blot Procedure
1. Run ~20 µg of Triton-treated porcine proximal tubule extract protein on a 7.5 % SDS-PAGE gel.*
. Transfer protein to the membrane using a Tris-Glycine/Methanol buffer.
. Block membrane with TBST/5 % NFDM for 30 min. at room temperature (~23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with TBST.
. Incubate membrane with 1:5,000 dilution of NB300-146 (anti-Na,K-ATPase), diluted in TBST, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with TBST.
. Incubate membrane with 1:15,000 dilution of goat anti-mouse IgG-HRP [(, diluted in TBST, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with TBST.
. Detect cross-reacting proteins using Chemiluminescent substrate according to manufacturers guidelines.* NOTE: Do not boil the protein samples, as boiling causes aggregation of the Na,K-ATPase. The aggregate band will appear at ~150 kDa on Western Blots.II. Quench Endogenous Peroxidase: IHC-FFPE sections
. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.
. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.- To Prepare 200 mL of Quenching Solution:- Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.- Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.
. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Buffer Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-ATP1A1 antibody (ATPase, Na+/K+ Transporting, alpha 1 Polypeptide) (ABIN152492) Western Blot analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule prote...
Immunohistochemistry (IHC) image for anti-ATP1A1 antibody (ATPase, Na+/K+ Transporting, alpha 1 Polypeptide) (ABIN152492) Staining of enodmetrial glands within the uterus using ABIN152492. Note the absence o...
Flow Cytometry (FACS) image for anti-ATP1A1 antibody (ATPase, Na+/K+ Transporting, alpha 1 Polypeptide) (ABIN152492) Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [ABIN152492] - Detec...
Western Blotting (WB) image for anti-ATP1A1 antibody (ATPase, Na+/K+ Transporting, alpha 1 Polypeptide) (ABIN152492) Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [ABIN152492] - Detecti...
Immunofluorescence (IF) image for anti-ATP1A1 antibody (ATPase, Na+/K+ Transporting, alpha 1 Polypeptide) (ABIN152492) Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464...
Product cited in: Liu, Edin, Blom, Magnusson, Schrott-Fischer, Glueckert, Santi, Li, Laurell, Rask-Andersen: "Super-resolution structured illumination fluorescence microscopy of the lateral wall of the cochlea: the Connexin26/30 proteins are separately expressed in man." in: Cell and tissue research, Vol. 365, Issue 1, pp. 13-27, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)

Strazielle, Creidy, Malcus, Boucraut, Ghersi-Egea: "T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium." in: PLoS ONE, Vol. 11, Issue 3, pp. e0150945, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Jang, Oh, Wada, Rowe, Liu, Chan, Rhee, Hoshino, Kim, Ibrahim, Baca, Kim, Ghosh, Parikh, Jiang, Chu, Forman, Lecker, Krishnaiah, Rabinowitz, Weljie, Baur, Kasper, Arany: "A branched-chain amino acid metabolite drives vascular fatty acid transport and causes insulin resistance." in: Nature medicine, Vol. 22, Issue 4, pp. 421-6, 2016 (PubMed).

Jiang, Woosley, Sivalingam, Natarajan, Howe: "Cathepsin-B-mediated cleavage of Disabled-2 regulates TGF-β-induced autophagy." in: Nature cell biology, Vol. 18, Issue 8, pp. 851-63, 2016 (PubMed).

Luo, Thaete, Neerhof: "Endothelin Receptor A Antagonism and Fetal Growth in Endothelial Nitric Oxide Synthase Gene Knockout Maternal and Fetal Mice." in: Reproductive sciences (Thousand Oaks, Calif.), Vol. 23, Issue 8, pp. 1028-36, 2016 (PubMed). Method employed by authors: Immunofluorescence (IF)

Paparelli, Corthout, Pavie, Wakefield, Sannerud, Jovanovic-Talisman, Annaert, Munck: "Inhomogeneity Based Characterization of Distribution Patterns on the Plasma Membrane." in: PLoS computational biology, Vol. 12, Issue 9, pp. e1005095, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Sugio, Tohyama, Oku, Fujiyoshi, Yoshimura, Hikishima, Yano, Fukuda, Nakamura, Okano, Watanabe, Fukata, Ikenaka, F Tanaka: "Astrocyte-mediated infantile-onset leukoencephalopathy mouse model." in: Glia, Vol. 65, Issue 1, pp. 150-168, 2016 (PubMed).

Lobato-Álvarez, Roldán, López-Murillo, González-Ramírez, Bonilla-Delgado, Shoshani: "The Apical Localization of Na(+), K(+)-ATPase in Cultured Human Retinal Pigment Epithelial Cells Depends on Expression of the β2 Subunit." in: Frontiers in physiology, Vol. 7, pp. 450, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Sütt, Altpere, Reimets, Visnapuu, Loomets, Raud, Salum, Mahlapuu, Kairane, Zilmer, Vasar: "Wfs1-deficient animals have brain-region-specific changes of Na+, K+-ATPase activity and mRNA expression of α1 and β1 subunits." in: Journal of neuroscience research, Vol. 93, Issue 3, pp. 530-7, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Huang, Wang, Deng: "Protective effects of dexamethasone on early acute lung injury induced by oleic acid in rats." in: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 4698-709, 2015 (PubMed). (Sample species: Rat (Rattus)).

Yan, Tang, Tan, Han, Zhou, Kang, Wang: "Proteomic Analysis of Isolated Plasma Membrane Fractions from the Mammary Gland in Lactating Cows." in: Journal of agricultural and food chemistry, Vol. 63, Issue 33, pp. 7388-98, 2015 (PubMed). (Sample species: Cow (Bovine)). Further details: Western Blotting

Tokuda, Kim, Matsumoto, Muro, Hirai, Mishima, Furuse: "Effects of Hydrostatic Pressure on Carcinogenic Properties of Epithelia." in: PLoS ONE, Vol. 10, Issue 12, pp. e0145522, 2015 (PubMed).

Yoon, Lim, Piao, Song, Kim, Yang: "Statin upregulates the expression of klotho, an anti-aging gene, in experimental cyclosporine nephropathy." in: Nephron. Experimental nephrology, Vol. 120, Issue 4, pp. e123-33, 2014 (PubMed).

Wang, Schultz: "Cholera toxin enhances Na(+) absorption across MCF10A human mammary epithelia." in: American journal of physiology. Cell physiology, Vol. 306, Issue 5, pp. C471-84, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Fan, Sun, Wang, Zhang, Xia, Peng, Hou, Shen, Liu, Peng: "Midkine, a potential link between obesity and insulin resistance." in: PLoS ONE, Vol. 9, Issue 2, pp. e88299, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Ohtake, Saito, Eto, Seki: "Exendin-4 promotes the membrane trafficking of the AMPA receptor GluR1 subunit and ADAM10 in the mouse neocortex." in: Regulatory peptides, Vol. 190-191, pp. 1-11, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Lungkaphin, Arjinajarn, Pongchaidecha, Srimaroeng, Chatsudthipong, Chatsudthipong: "Impaired insulin signaling affects renal organic anion transporter 3 (Oat3) function in streptozotocin-induced diabetic rats." in: PLoS ONE, Vol. 9, Issue 5, pp. e96236, 2014 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Hatou, Higa, Inagaki, Yoshida, Kimura, Hayashi, Tsujikawa, Tsubota, Nishida, Shimmura: "Validation of Na,K-ATPase pump function of corneal endothelial cells for corneal regenerative medicine." in: Tissue engineering. Part C, Methods, Vol. 19, Issue 12, pp. 901-10, 2013 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Feduska, Garcia, Brennan, Bu, Council, Yoon: "N-glycosylation of ICAM-2 is required for ICAM-2-mediated complete suppression of metastatic potential of SK-N-AS neuroblastoma cells." in: BMC cancer, Vol. 13, pp. 261, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Raksaseri, Chatsudthipong, Muanprasat, Soodvilai: "Activation of liver X receptors reduces CFTR-mediated Cl(-) transport in kidney collecting duct cells." in: American journal of physiology. Renal physiology, Vol. 305, Issue 4, pp. F583-91, 2013 (PubMed). (Sample species: Dog (Canine)). Further details: Western Blotting