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tyrosine Hydroxylase (TH) (pSer40) antibody

Details for Product No. ABIN152518, Supplier: Log in to see
Antigen
  • CG10118
  • DH65B
  • DTH
  • Dmel\\CG10118
  • Pale
  • Ple
  • TH
  • Th
  • VII
  • dTH1
  • dTH65B
  • pale/ple
  • th
  • 14-3-3
  • 14-3-3 epsilon
  • 14-3-3-e
  • 14-3-3-epsilon
  • 14-3-30epsilon
  • 14-3-3EPSILON
  • 14-3-3e
  • 14-3-3omicron
  • 1433epsilon
  • 143E_DROME
  • CG31196
  • CT24092
  • D14-3-3e
  • D14-3-3epsilon
  • Dmel\\CG31196
  • EK3-5
  • PAR-5
  • PAR5
  • Par-5
  • SR3-9
  • Su(Raf)3B
  • anon-WO0172774.141
  • anon-WO02059370.52
  • d14-3-3epsilon
  • eps
  • l(3)j2B10
  • par-5
  • DYT14
  • DYT5b
  • TYH
  • The
  • pale
  • tyrosine 3-monooxygenase
  • CG31196 gene product from transcript CG31196-RB
  • tyrosine hydroxylase
  • ple
  • th
  • Tsp_12013
  • 14-3-3epsilon
  • TH
  • Th
Epitope
pSer40
55
47
37
33
28
14
5
5
5
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
279
252
131
23
15
12
7
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
246
107
17
15
8
Clonality
Polyclonal
Conjugate
Un-conjugated
7
6
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
317
201
197
92
78
66
65
53
32
11
7
4
4
4
2
2
1
Options
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase.
Specificity Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.
Cross-Reactivity (Details) Reactivity assumed based on sequence identity to a wide variety of mammalian,non-mammalian species.
Purification Immunogen affinity purified
Alternative Name Tyrosine Hydroxylase (TH Antibody Abstract)
Background Gene Symbol: TH
Molecular Weight Theoretical MW: 60 kDa
Gene ID 7054
UniProt P04177
Research Area Enzymes, Amino Acids, Cancer
Application Notes Western Blot 1:1000, Immunohistochemistry 1:1000, Immunocytochemistry/Immunofluorescence 1:1000, Immunohistochemistry-Frozen 1:1000It has specifically been tested in PC12 lysates stimulated by okadaic acid. Depending upon brain region being studied, protein loads, and detection methods used, some higher bands may be detected.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer 10 mM HEPES ( pH 7.5), 0.15M NaCl, 0.1 mg/mL BSA and 50 % Glycerol
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-tyrosine Hydroxylase (TH) (pSer40) antibody (ABIN152518) anti-tyrosine Hydroxylase (TH) (pSer40) antibody
Immunohistochemistry (IHC) image for anti-tyrosine Hydroxylase (TH) (pSer40) antibody (ABIN152518) Immunohistochemistry: Tyrosine Hydroxylase [p Ser40] Antibody [ABIN152518] - Immunohi...
Western Blotting (WB) image for anti-tyrosine Hydroxylase (TH) (pSer40) antibody (ABIN152518) Western Blot: Tyrosine Hydroxylase [p Ser40] Antibody [ABIN152518] - Western blot ana...
Product cited in: Gassmann, Pfistner, Doan, Vogel, Soliz: "Impaired ventilatory acclimatization to hypoxia in female mice overexpressing erythropoietin: unexpected deleterious effect of estradiol in carotid bodies." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 299, Issue 6, pp. R1511-20, 2010 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Soliz, Joseph, Soulage, Becskei, Vogel, Pequignot, Ogunshola, Gassmann: "Erythropoietin regulates hypoxic ventilation in mice by interacting with brainstem and carotid bodies." in: The Journal of physiology, Vol. 568, Issue Pt 2, pp. 559-71, 2005 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Salvatore, Garcia-Espana, Goldstein, Deutch, Haycock: "Stoichiometry of tyrosine hydroxylase phosphorylation in the nigrostriatal and mesolimbic systems in vivo: effects of acute haloperidol and related compounds." in: Journal of neurochemistry, Vol. 75, Issue 1, pp. 225-32, 2000 (PubMed).

Witkovsky, Gabriel, Haycock, Meller: "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina." in: Journal of chemical neuroanatomy, Vol. 19, Issue 2, pp. 105-16, 2000 (PubMed). Further details: Immunohistochemistry (Frozen Sections)

Lew, Garcia-Espana, Lee, Carr, Goldstein, Haycock, Meller: "Increased site-specific phosphorylation of tyrosine hydroxylase accompanies stimulation of enzymatic activity induced by cessation of dopamine neuronal activity." in: Molecular pharmacology, Vol. 55, Issue 2, pp. 202-9, 1999 (PubMed).