tyrosine Hydroxylase (TH) (pSer40) antibody

Details for Product No. ABIN152518
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Antigen
Synonyms
CG10118, DH65B, DTH, Dmel\\CG10118, Pale, Ple, TH, Th, VII, dTH1, dTH65B, pale/ple, th, 14-3-3, 14-3-3 epsilon, 14-3-3-e, 14-3-3-epsilon, 14-3-30epsilon, 14-3-3EPSILON, 14-3-3e, 14-3-3omicron, 1433eps ... show more
CG10118, DH65B, DTH, Dmel\\CG10118, Pale, Ple, TH, Th, VII, dTH1, dTH65B, pale/ple, th, 14-3-3, 14-3-3 epsilon, 14-3-3-e, 14-3-3-epsilon, 14-3-30epsilon, 14-3-3EPSILON, 14-3-3e, 14-3-3omicron, 1433epsilon, 143E_DROME, CG31196, CT24092, D14-3-3e, D14-3-3epsilon, Dmel\\CG31196, EK3-5, PAR-5, PAR5, Par-5, SR3-9, Su(Raf)3B, anon-WO0172774.141, anon-WO02059370.52, d14-3-3epsilon, eps, l(3)j2B10, par-5, DYT14, DYT5b, TYH, The show less
Epitope
pSer40
(45), (35), (34), (22), (16), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(190), (165), (125), (16), (15), (14), (8), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(228), (35), (12), (8), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))
(229), (132), (121), (72), (59), (53), (38), (30), (21), (6), (4)
Pubmed 3 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN152518
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase.
Specificity Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.
Cross-Reactivity (Details) Reactivity assumed based on sequence identity to a wide variety of mammalian,non-mammalian species.
Purification Affinity purified
Alternative Name Tyrosine 3-monooxygenase (TH)
Background Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of thecatecholamines dopamine, epinephrine and norepinephrine. Therefore, the regulationof the TH enzyme represents the central means for controlling the synthesis of theseimportant catecholamines. TH has a large molecular diversity, resulting from differentialsplicing of its mRNA, which is tissue specific and might result in long term changes inactivity of the enzyme and its availability of neurotransmitter at various synapses. The presence of different DNA sequences at the TH locus confers susceptibility tovarious disorders of the brain including manic-depression and schizophrenia. Parkinson',s disease is also considered a TH deficiency, as low dopamine levelsare a consistent neurochemical abnormality. Alternate Names: anti-TH antibody, anti-Tyrosine hydroxylase antibody, anti-Tyrosine 3-monooxygenaseantibody, anti-L-tyrosine hydroxylase antibody, anti-Tyrosine 3-hydroxylase antibody. Related Diseases: Manic depression, Schizophrenia, Parkinson',s disease
Gene Symbol: TH
Gene ID 7054, 21823, 25085
Research Area Amino Acids, Enzymes, Phospho-specific antibodies
Application Notes It has specifically been tested in PC12 lysates stimulated by okadaic acid. Depending upon brain region being studied, protein loads, and detection methods used, some higher bands may be detected.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:1000, Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer 10 mM HEPES [pH 7.5] 150 mM NaCl, 100 ug per ml BSA and 50 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-tyrosine Hydroxylase (TH) (pSer40) antibody anti-tyrosine Hydroxylase (TH) (pSer40) antibody
General Lew, Garcia-Espana, Lee et al.: "Increased site-specific phosphorylation of tyrosine hydroxylase accompanies stimulation of enzymatic activity induced by cessation of dopamine neuronal activity." in: Molecular pharmacology, Vol. 55, Issue 2, pp. 202-9, 1999 (PubMed).

Salvatore, Garcia-Espana, Goldstein et al.: "Stoichiometry of tyrosine hydroxylase phosphorylation in the nigrostriatal and mesolimbic systems in vivo: effects of acute haloperidol and related compounds." in: Journal of neurochemistry, Vol. 75, Issue 1, pp. 225-32, 2000 (PubMed).

Witkovsky, Gabriel, Haycock et al.: "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina." in: Journal of chemical neuroanatomy, Vol. 19, Issue 2, pp. 105-16, 2000 (PubMed).

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