Protein tyrosine Phosphatase, Non-Receptor Type 5 (Striatum-Enriched) (PTPN5) (AA 57-74) antibody

Details for Product No. ABIN152543
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Antigen
Synonyms STEP, Step, PTPSTEP
Epitope
AA 57-74
(19), (7), (3), (2), (2), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(49), (23), (22), (1), (1), (1), (1), (1)
Host
Mouse
(40), (14)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(43), (19), (10), (9), (8), (4), (2), (2), (1), (1)
Pubmed 7 references available
Quantity 0.1 mL
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Catalog No. ABIN152543
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Immunogen A synthetic peptide of rat STEP46 (amino acids 57-74: MCTPGCNEEGFGYLVSPR)
Clone 23E5
Isotype IgG1
Specificity NB300-202 is specific for STEP61, STEP46, STEP38, and STEP20.
Purification Ascites
Alternative Name PTPN5 / STEP
Background Tyrosine phosphorylation plays a critical role in many physiological processes. There isa fine balance between phosphorylation caused by protein tyrosine kinases and actionsof protein tyrosine phosphatases, that regulates these physiological processes. Striatalenriched phosphatase (STEP) is part of a family of protein tyrosine phosphatasesenriched within the CNS and is a component of the NMDAR complex. It is expressedwithin dopaminoceptive neurons in the CNS and particularly enriched within the basalganglia and related structures.STEP61 is a membrane-associated isoform and STEP46 is a cytosolic protein. Inresponse to transient hypoxia-ischemia in the brain, STEP61 is proteolytically cleavedby calpain, thereby releasing a smaller isoform, STEP33. Alternate Names: Anti-FLJ14427 antibody, Anti-Neural specific protein tyrosine phosphatase antibody,Anti-Protein tyrosine phosphatase non receptor type 5 (striatum enriched) antibody,Anti-Protein tyrosine phosphatase non receptor type 5 antibody, Anti-Protein tyrosinephosphatase striatum enriched antibody, Anti-PTN5 antibody, Anti-PTP STEP antibody,Anti-PTPN 5 antibody, Anti-PTPSTEP antibody, Anti-Tyrosine protein phosphatase nonreceptor type 5 antibody, anti-PTPN5 antibody.
Gene Symbol: PTPN5
Gene ID 84867, 29644
Application Notes This STEP (23E5) antibody is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. By Western Blot, this STEP antibody recognizes bands at ~64-66, 46, 37, and 33 kDa, in rat brain tissues. Neuronal cells have been used for ICC/IF and frozen brain sections have been used for IHC.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunohistochemistry 1:100-1:500, Immunohistochemistry-Frozen 1:100-1:500, immunoprecipitation 1:50, Western Blot 1:1000 -1:2000
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-Protein tyrosine Phosphatase, Non-Receptor Type 5 (Striatum-Enriched) (PTPN5) (AA 57-74) antibody Western Blot analysis of STEP protein in striatal rat protein homogenates, using ABIN152543.
General Lombroso, Murdoch, Lerner: "Molecular characterization of a protein-tyrosine-phosphatase enriched in striatum." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 16, pp. 7242-6, 1991 (PubMed).

Sharma, Zhao, Bult et al.: "Identification of two alternatively spliced transcripts of STEP: a subfamily of brain-enriched protein tyrosine phosphatases." in: Brain research. Molecular brain research, Vol. 32, Issue 1, pp. 87-93, 1996 (PubMed).

Boulanger, Lombroso, Raghunathan et al.: "Cellular and molecular characterization of a brain-enriched protein tyrosine phosphatase." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 2, pp. 1532-44, 1995 (PubMed).

Lombroso, Naegele, Sharma et al.: "A protein tyrosine phosphatase expressed within dopaminoceptive neurons of the basal ganglia and related structures." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 13, Issue 7, pp. 3064-74, 1993 (PubMed).

Choi, Lin, Lee et al.: "Status epilepticus-induced somatostatinergic hilar interneuron degeneration is regulated by striatal enriched protein tyrosine phosphatase." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 11, pp. 2999-3009, 2007 (PubMed).

Zhao, Choi, Obrietan et al.: "Synaptic plasticity (and the lack thereof) in hippocampal CA2 neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 44, pp. 12025-32, 2007 (PubMed).

DiRocco, Scheiner, Sindreu et al.: "A role for calmodulin-stimulated adenylyl cyclases in cocaine sensitization." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 8, pp. 2393-403, 2009 (PubMed).

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