Protein tyrosine Phosphatase, Non-Receptor Type 5 (Striatum-Enriched) (PTPN5) antibody

Antigen: Protein tyrosine Phosphatase, Non-Receptor Type 5 (Striatum-Enriched) (PTPN5)

Clonality: Monoclonal (23E5)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN152543

Additional Information

Alternative name: PTPN5 / STEP

Gene ID: 84867, 29644

Immunogen: A synthetic peptide of STEP46 (amino acids 57-74: MCTPGCNEEGFGYLVSPR).

Cross-Reactivity: Rat (Rattus)

Format: Ascites

Isotype: IgG

Clone: 23E5

Description: Tyrosine phosphorylation plays a critical role in many physiological processes. There isa fine balance between phosphorylation caused by protein tyrosine kinases and actionsof protein tyrosine phosphatases, that regulates these physiological processes. Striatalenriched phosphatase (STEP) is part of a family of protein tyrosine phosphatasesenriched within the CNS and is a component of the NMDAR complex. It is expressedwithin dopaminoceptive neurons in the CNS and particularly enriched within the basalganglia and related structures.STEP61 is a membrane-associated isoform and STEP46 is a cytosolic protein. Inresponse to transient hypoxia-ischemia in the brain, STEP61 is proteolytically cleavedby calpain, thereby releasing a smaller isoform, STEP33. Alternate Names: Anti-FLJ14427 antibody, Anti-Neural specific protein tyrosine phosphatase antibody,Anti-Protein tyrosine phosphatase non receptor type 5 (striatum enriched) antibody,Anti-Protein tyrosine phosphatase non receptor type 5 antibody, Anti-Protein tyrosinephosphatase striatum enriched antibody, Anti-PTN5 antibody, Anti-PTP STEP antibody,Anti-PTPN 5 antibody, Anti-PTPSTEP antibody, Anti-Tyrosine protein phosphatase nonreceptor type 5 antibody, anti-PTPN5 antibody.

Specificity: ABIN152543 is specific for STEP61, STEP46, STEP38, and STEP20. Species Reactivity: ABIN152543 recognizes rat STEP proteins. Other species have not been tested. However, there is 100% homology between the mouse protein and the immunogenused for this antibody production. There is 94% homology between the human proteinand the immunogen used for this antibody production. Localization: Cytoplasmic.

Application Details

Application Notes: WB 1:1000-1:2000. Neuronal cells have been used for immunocytochemistry and frozen brain sections have been used for immunohistochemistry with past lots. This lot has not yet been tested for these two applications. Positive Controls: Rat brain tissue

Concentration: 9 mg/ml

Purification: Ascites

Buffer: Preservative: 0.09% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

Western Blot analysis of STEP protein in striatal rat protein homogenates, using ABIN152543.

Publications

Lombroso, Murdoch, Lerner: "Molecular characterization of a protein-tyrosine-phosphatase enriched in striatum." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 16, pp. 7242-6, 1991 (PubMed).

Sharma, Zhao, Bult et al.: "Identification of two alternatively spliced transcripts of STEP: a subfamily of brain-enriched protein tyrosine phosphatases." in: Brain research. Molecular brain research, Vol. 32, Issue 1, pp. 87-93, 1996 (PubMed).

Boulanger, Lombroso, Raghunathan et al.: "Cellular and molecular characterization of a brain-enriched protein tyrosine phosphatase." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 2, pp. 1532-44, 1995 (PubMed).

Lombroso, Naegele, Sharma et al.: "A protein tyrosine phosphatase expressed within dopaminoceptive neurons of the basal ganglia and related structures." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 13, Issue 7, pp. 3064-74, 1993 (PubMed).

Choi, Lin, Lee et al.: "Status epilepticus-induced somatostatinergic hilar interneuron degeneration is regulated by striatal enriched protein tyrosine phosphatase." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 11, pp. 2999-3009, 2007 (PubMed).

Zhao, Choi, Obrietan et al.: "Synaptic plasticity (and the lack thereof) in hippocampal CA2 neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 44, pp. 12025-32, 2007 (PubMed).

DiRocco, Scheiner, Sindreu et al.: "A role for calmodulin-stimulated adenylyl cyclases in cocaine sensitization." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 8, pp. 2393-403, 2009 (PubMed).