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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Protein tyrosine Phosphatase, Non-Receptor Type 5 (Striatum-Enriched) (PTPN5) antibody
Human, Mouse (Murine), Rat (Rattus)
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|7 references available|
|Quantity||0.1 mL (9 mg/ml)|
|Price||Product not available in this region.|
|Alternative name||PTPN5 / STEP|
|Gene ID||84867, 29644|
|Immunogen||A synthetic peptide of STEP46 (amino acids 57-74: MCTPGCNEEGFGYLVSPR).|
|Description||Tyrosine phosphorylation plays a critical role in many physiological processes. There isa fine balance between phosphorylation caused by protein tyrosine kinases and actionsof protein tyrosine phosphatases, that regulates these physiological processes. Striatalenriched phosphatase (STEP) is part of a family of protein tyrosine phosphatasesenriched within the CNS and is a component of the NMDAR complex. It is expressedwithin dopaminoceptive neurons in the CNS and particularly enriched within the basalganglia and related structures.STEP61 is a membrane-associated isoform and STEP46 is a cytosolic protein. Inresponse to transient hypoxia-ischemia in the brain, STEP61 is proteolytically cleavedby calpain, thereby releasing a smaller isoform, STEP33. Alternate Names: Anti-FLJ14427 antibody, Anti-Neural specific protein tyrosine phosphatase antibody,Anti-Protein tyrosine phosphatase non receptor type 5 (striatum enriched) antibody,Anti-Protein tyrosine phosphatase non receptor type 5 antibody, Anti-Protein tyrosinephosphatase striatum enriched antibody, Anti-PTN5 antibody, Anti-PTP STEP antibody,Anti-PTPN 5 antibody, Anti-PTPSTEP antibody, Anti-Tyrosine protein phosphatase nonreceptor type 5 antibody, anti-PTPN5 antibody.|
|Specificity||ABIN152543 is specific for STEP61, STEP46, STEP38, and STEP20. Species Reactivity: ABIN152543 recognizes rat STEP proteins. Other species have not been tested. However, there is 100% homology between the mouse protein and the immunogenused for this antibody production. There is 94% homology between the human proteinand the immunogen used for this antibody production. Localization: Cytoplasmic.|
|Application Notes||WB 1:1000-1:2000. Neuronal cells have been used for immunocytochemistry and frozen brain sections have been used for immunohistochemistry with past lots. This lot has not yet been tested for these two applications. Positive Controls: Rat brain tissue|
|Buffer||Preservative: 0.09% sodium azide.|
|Storage||Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.|
|Restrictions||For Research Use only|
|Western Blot analysis of STEP protein in striatal rat protein homogenates, using ABIN152543.|
Lombroso, Murdoch, Lerner: "Molecular characterization of a protein-tyrosine-phosphatase enriched in striatum." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 16, pp. 7242-6, 1991 (PubMed).
Sharma, Zhao, Bult et al.: "Identification of two alternatively spliced transcripts of STEP: a subfamily of brain-enriched protein tyrosine phosphatases." in: Brain research. Molecular brain research, Vol. 32, Issue 1, pp. 87-93, 1996 (PubMed).
Boulanger, Lombroso, Raghunathan et al.: "Cellular and molecular characterization of a brain-enriched protein tyrosine phosphatase." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 2, pp. 1532-44, 1995 (PubMed).
Lombroso, Naegele, Sharma et al.: "A protein tyrosine phosphatase expressed within dopaminoceptive neurons of the basal ganglia and related structures." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 13, Issue 7, pp. 3064-74, 1993 (PubMed).
Choi, Lin, Lee et al.: "Status epilepticus-induced somatostatinergic hilar interneuron degeneration is regulated by striatal enriched protein tyrosine phosphatase." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 11, pp. 2999-3009, 2007 (PubMed).
Zhao, Choi, Obrietan et al.: "Synaptic plasticity (and the lack thereof) in hippocampal CA2 neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 44, pp. 12025-32, 2007 (PubMed).
DiRocco, Scheiner, Sindreu et al.: "A role for calmodulin-stimulated adenylyl cyclases in cocaine sensitization." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 8, pp. 2393-403, 2009 (PubMed).
|Hosts||Rabbit (8), Mouse (5)|
|Reactivities||Human (6), Rat (Rattus) (5), Mouse (Murine) (2), Chicken (1), Cow (Bovine) (1), Fruit Fly (Drosophila melanogaster) (1), Xenopus laevis (1), Zebrafish (1)|
|Applications||Western Blotting (WB) (12), ELISA (3), Immunohistochemistry (IHC) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Immunohistochemistry (Fixed) (IHC (fx)) (1)|
|Epitopes||AA 57-74 (2), N-Term (2), Internal Region (1)|