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Protein tyrosine Phosphatase, Non-Receptor Type 5 (Striatum-Enriched) (PTPN5) antibody

Details for Product No. ABIN152543, Supplier: Log in to see
Antigen
  • STEP
  • Step
  • PTPSTEP
  • protein tyrosine phosphatase, non-receptor type 5
  • protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched)
  • ptpn5
  • PTPN5
  • Ptpn5
Reactivity
Human, Mouse (Murine), Rat (Rattus)
86
34
28
2
2
2
1
1
1
1
1
1
1
1
1
1
Host
Mouse
75
12
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
7
7
7
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
64
41
19
14
13
4
3
2
1
Options
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Immunogen A synthetic peptide of rat STEP46 (amino acids 57-74: MCTPGCNEEGFGYLVSPR)
Clone 23E5
Isotype IgG1
Specificity This antibody is specific for STEP61, STEP46, STEP38, and STEP20.
Purification Unpurified
Alternative Name STEP (PTPN5 Antibody Abstract)
Background Gene Symbol: PTPN5
Gene ID 84867
Application Notes Western Blot 1:1000 -1:2000, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunoprecipitation 1:50, Immunohistochemistry-Frozen 1:100-1:500This STEP (23E5) antibody is useful for Western blot, Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. By Western Blot, this STEP antibody recognizes bands at ~64-66, 46, 37, and 33 kDa, in rat brain tissues. Neuronal cells have been used for ICC/IF and frozen brain sections have been used for IHC.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer Ascites
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Protein tyrosine Phosphatase, Non-Receptor Type 5 (Striatum-Enriched) (PTPN5) antibody (ABIN152543) Western Blot analysis of STEP protein in striatal rat protein homogenates, using ABIN...
Product cited in: Poddar, Rajagopal, Shuttleworth, Paul: "Zn2+-dependent Activation of the Trk Signaling Pathway Induces Phosphorylation of the Brain-enriched Tyrosine Phosphatase STEP: MOLECULAR BASIS FOR ZN2+-INDUCED ERK MAPK ACTIVATION." in: The Journal of biological chemistry, Vol. 291, Issue 2, pp. 813-25, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting,Immunoprecipitation

Rajagopal, Deb, Poddar, Paul: "Aging is associated with dimerization and inactivation of the brain-enriched tyrosine phosphatase STEP." in: Neurobiology of aging, Vol. 41, pp. 25-38, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunoprecipitation

Won, Incontro, Nicoll, Roche: "PSD-95 stabilizes NMDA receptors by inducing the degradation of STEP61." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, Issue 32, pp. E4736-44, 2016 (PubMed). Further details: Western Blotting

Kim, Jang, Choi: "A single fraction from Uncaria sinensis exerts neuroprotective effects against glutamate-induced neurotoxicity in primary cultured cortical neurons." in: Anatomy & cell biology, Vol. 48, Issue 2, pp. 95-103, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Sun, Zelek-Molik, McGinty: "Short and long access to cocaine self-administration activates tyrosine phosphatase STEP and attenuates GluN expression but differentially regulates GluA expression in the prefrontal cortex." in: Psychopharmacology, Vol. 229, Issue 4, pp. 603-13, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunoprecipitation

Kim, Kim, Jang, Choi, Baek, Hong, Choi, Shin, Choi: "Neuroprotective effects of Polygonum multiflorum extract against glutamate-induced oxidative toxicity in HT22 hippocampal cells." in: Journal of ethnopharmacology, Vol. 150, Issue 1, pp. 108-15, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Deb, Manhas, Poddar, Rajagopal, Allan, Lombroso, Rosenberg, Candelario-Jalil, Paul: "Neuroprotective role of a brain-enriched tyrosine phosphatase, STEP, in focal cerebral ischemia." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 45, pp. 17814-26, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting,Immunoprecipitation

Wang, Briz, Chishti, Bi, Baudry: "Distinct roles for μ-calpain and m-calpain in synaptic NMDAR-mediated neuroprotection and extrasynaptic NMDAR-mediated neurodegeneration." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 48, pp. 18880-92, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Davis, Olausson, Greengard, Taylor, Nairn: "Regulator of calmodulin signaling knockout mice display anxiety-like behavior and motivational deficits." in: The European journal of neuroscience, Vol. 35, Issue 2, pp. 300-8, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Farooq, Kaswala, Kleiman, Kasinathan, Frederikse: "GluA2 AMPA glutamate receptor subunit exhibits codon 607 Q/R RNA editing in the lens." in: Biochemical and biophysical research communications, Vol. 418, Issue 2, pp. 273-7, 2012 (PubMed).

Shinohara, Hosoya, Yahagi, Ferecskó, Yaguchi, Sík, Itakura, Takahashi, Hirase: "Hippocampal CA3 and CA2 have distinct bilateral innervation patterns to CA1 in rodents." in: The European journal of neuroscience, Vol. 35, Issue 5, pp. 702-10, 2012 (PubMed).

Mukherjee, Poddar, Deb, Paul: "Dephosphorylation of specific sites in the kinase-specificity sequence domain leads to ubiquitin-mediated degradation of the tyrosine phosphatase STEP." in: The Biochemical journal, Vol. 440, Issue 1, pp. 115-25, 2011 (PubMed).

Zhang, Kurup, Xu, Carty, Fernandez, Nygaard, Pittenger, Greengard, Strittmatter, Nairn, Lombroso: "Genetic reduction of striatal-enriched tyrosine phosphatase (STEP) reverses cognitive and cellular deficits in an Alzheimer's disease mouse model." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19014-9, 2010 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation

Poddar, Deb, Mukherjee, Paul: "NR2B-NMDA receptor mediated modulation of the tyrosine phosphatase STEP regulates glutamate induced neuronal cell death." in: Journal of neurochemistry, Vol. 115, Issue 6, pp. 1350-62, 2010 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Kurup, Zhang, Xu, Venkitaramani, Haroutunian, Greengard, Nairn, Lombroso: "Abeta-mediated NMDA receptor endocytosis in Alzheimer's disease involves ubiquitination of the tyrosine phosphatase STEP61." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 17, pp. 5948-57, 2010 (PubMed). Further details: Western Blotting

DiRocco, Scheiner, Sindreu, Chan, Storm: "A role for calmodulin-stimulated adenylyl cyclases in cocaine sensitization." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 8, pp. 2393-403, 2009 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Goebel-Goody, Davies, Alvestad Linger, Freund, Browning: "Phospho-regulation of synaptic and extrasynaptic N-methyl-d-aspartate receptors in adult hippocampal slices." in: Neuroscience, Vol. 158, Issue 4, pp. 1446-59, 2009 (PubMed).

Zhang, Venkitaramani, Gladding, Zhang, Kurup, Molnar, Collingridge, Lombroso: "The tyrosine phosphatase STEP mediates AMPA receptor endocytosis after metabotropic glutamate receptor stimulation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 42, pp. 10561-6, 2008 (PubMed). Further details: Western Blotting,Immunohistochemistry

Choi, Lin, Lee, Kurup, Cho, Naegele, Lombroso, Obrietan: "Status epilepticus-induced somatostatinergic hilar interneuron degeneration is regulated by striatal enriched protein tyrosine phosphatase." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 11, pp. 2999-3009, 2007 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Zhao, Choi, Obrietan, Dudek: "Synaptic plasticity (and the lack thereof) in hippocampal CA2 neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 44, pp. 12025-32, 2007 (PubMed).

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