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Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr305) antibody

Details for Product No. ABIN152549
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Antigen
Synonyms CAMKA, PKCCD, PK2CDD, zgc:112538, zgc:123320, camk2, camk2b
Epitope
pThr305
(22), (20), (7), (5), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(76), (66), (45), (9), (6), (6), (6), (5), (5), (5), (3), (2), (2), (1), (1), (1), (1)
Host
Rabbit
(75), (45), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(121), (64), (38), (34), (26), (24), (23), (6), (4), (3), (2), (2), (2)
Pubmed 1 reference available
Quantity 0.1 mL
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Catalog No. ABIN152549
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr305 found in rat brain CaM Kinase II.
Specificity Specific for the ~50k alpha-CaM Kinase II and the ~60k beta-CaM Kinase II proteins phosphorylated at Thr305. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
Cross-Reactivity (Details) Expected cross reactivity from sequence similarity: bovine, chicken, canine, human, mouse, Xenopus,zebra fish.
Purification Immunogen affinity purified
Alternative Name CAMK2A
Background CaM KII is an important member of calcium/calmodulin-activated kinase, functioning inneural synaptic stimulation and T-cell receptor signaling. CaM KII has catalytic andregulatory domains. The binding of Ca++/calmodulin to its regulatory domain releasesits autoinhibitory effect and activates the kinase. The activated CaM KII furtherautophosphorylates itself at Thr286 to render the kinase constitutively active. Thethreonine phosphorylation state of CaM KII can be regulated through PP1/PKA. PP1(protein phosphatase 1) dephosphorylates phospho-CaM KII at Thr286. On the otherhand, PKA (protein kinase A) prevents this phosphorylation process through itsinhibitory effect on PP1. Alternate Names: anti-Cam KII antibody, anti-CAMKII antibody.
Gene Symbol: CAMK2A
Gene ID 815, 12325, 171140
Application Notes NB 300-208 can be used in Western blot where a band is seen at ~ 50 kD and 60 kD, representing CAM KII. Immunofluorescence has not been determined. *The investigator should determine the optimal dilution for a specific application.
Recommended dilutions: Western Blot 1:1000
Protocol WB protocol specific for CaMKII alpha/beta Antibody
1. Thoroughly sonicate cell lysates or tissue homogenates to be loaded onto gel then dilute in appropriate sample buffer and boil for 5 minutes at 100C. Let samples cool to room temperature then load onto gel.
. Run SDS-PAGE per gel apparatus manufacturer'??s instructions.
. Transfer proteins to nitrocellulose or PVDF membrane (if using PVDF, be sure to activate membrane in Methanol prior to use).
. After transfer, air-dry blot to more stably fix proteins onto membrane.
. Block non-specific sites on membrane in 3 % BSA-TTBS (Tris-
Restrictions For Research Use only
Format Liquid
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr305) antibody anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr305) antibody
General Elgersma, Fedorov, Ikonen et al.: "Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning." in: Neuron, Vol. 36, Issue 3, pp. 493-505, 2002 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN152549
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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