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GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase)

Details for Product anti-GAPDH Antibody No. ABIN152561, Supplier: Log in to see
Antigen
  • BEST:GH12586
  • cb609
  • CG8893
  • CG12055
  • Dmel\\CG8893
  • Dmel\\CG12055
  • g3pd
  • G3PD
  • G3PDH
  • GA3PDH
  • GADPH
  • gadph
  • GAP
  • GAPC-2
  • gapd
  • GAPD
  • Gapd
  • gapdh
  • GAPDH
  • Gapdh
  • GAPDH-1
  • Gapdh-1
  • gapdh-1
  • Gapdh-2
  • GAPDH1
  • GAPDH2
  • Gapdh13F
  • Gapdh43E
  • GAPDHI
  • GAPDH I
  • GAPDH II
  • GB14798
  • gh12586
  • GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2
  • glyceraldehyde-3-phosphate dehydrogenase C2
  • KNC-NDS6
  • mg:bb02e05
  • T6J4.17
  • T6J4_17
  • wu:fb33a10
  • wu:ft80f05
Reactivity
Bird (Avian)
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271
141
105
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44
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34
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Host
Mouse
369
329
58
32
20
Clonality (Clone)
Monoclonal ()
Conjugate
This GAPDH antibody is un-conjugated
50
41
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Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
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1
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Supplier
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Immunogen Purified porcine GAPDH
Clone 1D4
Isotype IgM kappa
Cross-Reactivity (Details) Likely to react with other mammals as well. Hamster,zebrafish reactivity reported by a customer review. chicken reactivity reported in scientific literature (PMID: 24725420)
Purification Tissue culture supernatant
Alternative Name GAPDH (GAPDH Antibody Abstract)
Background GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible forcatalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation ofglyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amountsand which is required at all times for an important house keeping functions, levels ofGAPDH mRNA are often used as standards in studies of mRNA expression.Increasingly, scientists are making use of specific antibodies to GAPDH as loadingcontrols for western blotting experiments. Apart from a role in glycolysis, GAPDH mayhave other roles such as in the activation of transcription (1). GAPDH is reported to bindto a variety of other proteins, including the amyloid precursor protein, mutations in whichcause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin,the protein product aberrant forms of which are causative of Huntington's disease (2,3).Associations with actin and tubulin have also be reported. The protein may also have arole in the regulation of apoptosis, and interestingly migrates from the cytoplasm into thenucleus when cells become apoptotic (4). Alternate Names: anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody,anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685antibody.
Gene Symbol: GAPDH
Gene ID 2597, 14433, 24383
UniProt P00355
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Application Notes This GAPDH Antibody (1D4) is useful for Western blot and Immunocytochemistry/Immunofluorescence. Immunohistochemistry was reported in scientific literature. In WB a prominent band can be seen at approximately 38 kDa. This antibody makes an excellent loading control for Western blot experiments.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry, Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN152561) Sciatic nerves of mouse wild type (+/+), heterozygous (+/o) and homozygous (o/o) for ...
Immunofluorescence (IF) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN152561) Human neuroblastma line Sh-SY5Y stained with anti-GAPDH (ABIN152561) [green] and an a...
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN152561) Western Blot: GAPDH Antibody (1D4) [ABIN152561] - analysis of GAPDH in human nucleus ...
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN152561) Western Blot: GAPDH Antibody (1D4) [ABIN152561] - Zebrafish embryo lysates at differe...
Immunofluorescence (IF) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN152561) Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [ABIN152561] - SH-SY5Y c...
Western Blotting (WB) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN152561) Western Blot: GAPDH Antibody (1D4) [ABIN152561] - analysis of GAPDH in chicken pector...
Simple Western (SimWes) image for anti-GAPDH antibody (Glyceraldehyde-3-Phosphate Dehydrogenase) (ABIN152561) Simple Western: GAPDH Antibody (1D4) [ABIN152561] - Simple Western lane view shows a ...
Product cited in: Lassance, Miedl, Konya, Heinemann, Ebner, Hackl, Desoye, Hiden: "Differential response of arterial and venous endothelial cells to extracellular matrix is modulated by oxygen." in: Histochemistry and cell biology, Vol. 137, Issue 5, pp. 641-55, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Kokubun, Pankajakshan, Kim, Agrawal: "Differentiation of porcine mesenchymal stem cells into epithelial cells as a potential therapeutic application to facilitate epithelial regeneration." in: Journal of tissue engineering and regenerative medicine, Vol. 10, Issue 2, pp. E73-83, 2016 (PubMed). (Sample species: Pig (Porcine)).

Serbo, Kuo, Lewis, Lehmann, Li, Gracias, Romer: "Patterning of Fibroblast and Matrix Anisotropy within 3D Confinement is Driven by the Cytoskeleton." in: Advanced healthcare materials, Vol. 5, Issue 1, pp. 146-58, 2016 (PubMed).

Becs, Zarjou, Agarwal, Kovács, Becs, Nyitrai, Balogh, Bányai, Eaton, Arosio, Poli, Jeney, Balla, Balla: "Pharmacological induction of ferritin prevents osteoblastic transformation of smooth muscle cells." in: Journal of cellular and molecular medicine, Vol. 20, Issue 2, pp. 217-30, 2016 (PubMed).

Slocum, Skoko, Wakabayashi, Aja, Yamamoto, Kensler, Chartoumpekis: "Keap1/Nrf2 pathway activation leads to a repressed hepatic gluconeogenic and lipogenic program in mice on a high-fat diet." in: Archives of biochemistry and biophysics, Vol. 591, pp. 57-65, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Chen, Statt, Wu, Chang, Liao, Wang, Shyu, Lee: "High mobility group box 1-induced epithelial mesenchymal transition in human airway epithelial cells." in: Scientific reports, Vol. 6, pp. 18815, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Kümper, Mardakheh, McCarthy, Yeo, Stamp, Paul, Worboys, Sadok, Jørgensen, Guichard, Marshall: "Rho-associated kinase (ROCK) function is essential for cell cycle progression, senescence and tumorigenesis." in: eLife, Vol. 5, pp. e12994, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Krapp, Hotter, Gawanbacht, McLaren, Kluge, Stürzel, Mack, Reith, Engelhart, Ciuffi, Hornung, Sauter, Telenti, Kirchhoff: "Guanylate Binding Protein (GBP) 5 Is an Interferon-Inducible Inhibitor of HIV-1 Infectivity." in: Cell host & microbe, Vol. 19, Issue 4, pp. 504-14, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Ochaba, Monteys, ORourke, Reidling, Steffan, Davidson, Thompson: "PIAS1 Regulates Mutant Huntingtin Accumulation and Huntington's Disease-Associated Phenotypes In Vivo." in: Neuron, Vol. 90, Issue 3, pp. 507-20, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Drelon, Berthon, Sahut-Barnola, Mathieu, Dumontet, Rodriguez, Batisse-Lignier, Tabbal, Tauveron, Lefrançois-Martinez, Pointud, Gomez-Sanchez, Vainio, Shan, Sacco, Schedl, Stratakis, Martinez, Val: "PKA inhibits WNT signalling in adrenal cortex zonation and prevents malignant tumour development." in: Nature communications, Vol. 7, pp. 12751, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Ding, Paszkowski-Rogacz, Winzi, Chakraborty, Theis, Singh, Ciotta, Poser, Roguev, Chu, Choudhary, Mann, Stewart, Krogan, Buchholz: "Systems Analyses Reveal Shared and Diverse Attributes of Oct4 Regulation in Pluripotent Cells." in: Cell systems, Vol. 1, Issue 2, pp. 141-51, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Willi, Yoo, Wang, Trajanoski, Hennighausen: "Differential cytokine sensitivities of STAT5-dependent enhancers rely on Stat5 autoregulation." in: Nucleic acids research, Vol. 44, Issue 21, pp. 10277-10291, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Sandin, Bergkvist, Nath, Kielkopf, Janefjord, Helmfors, Zetterberg, Blennow, Li, Nilsberth, Garner, Brorsson, Kågedal: "Beneficial effects of increased lysozyme levels in Alzheimer's disease modelled in Drosophila melanogaster." in: The FEBS journal, Vol. 283, Issue 19, pp. 3508-3522, 2016 (PubMed).

Yoon, Storm, Kamimae-Lanning, Goloviznina, Kurre: "Endogenous DNA Damage Leads to p53-Independent Deficits in Replicative Fitness in Fetal Murine Fancd2(-/-) Hematopoietic Stem and Progenitor Cells." in: Stem cell reports, Vol. 7, Issue 5, pp. 840-853, 2016 (PubMed).

Zhao, Koenig, Trask, Lin, Hans, Garg, Lilly: "MicroRNA miR145 regulates TGFBR2 expression and matrix synthesis in vascular smooth muscle cells." in: Circulation research, Vol. 116, Issue 1, pp. 23-34, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Ye, Zhou, Markova, Tian, Li, Anderson, Shapiro, Risbud: "Xylosyltransferase-1 expression is refractory to inhibition by the inflammatory cytokines tumor necrosis factor α and IL-1β in nucleus pulposus cells: novel regulation by AP-1, Sp1, and Sp3." in: The American journal of pathology, Vol. 185, Issue 2, pp. 485-95, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Ikhapoh, Pelham, Agrawal: "Synergistic effect of angiotensin II on vascular endothelial growth factor-A-mediated differentiation of bone marrow-derived mesenchymal stem cells into endothelial cells." in: Stem cell research & therapy, Vol. 6, pp. 4, 2015 (PubMed).

Kutz, Greaney, Santhanam, Alexander: "Evidence for a functional vasodilatatory role for hydrogen sulphide in the human cutaneous microvasculature." in: The Journal of physiology, Vol. 593, Issue 9, pp. 2121-9, 2015 (PubMed). Further details: Western Blotting

De Boussac, Maqdasy, Trousson, Zelcer, Volle, Lobaccaro, Baron: "Enolase is regulated by Liver X Receptors." in: Steroids, Vol. 99, Issue Pt B, pp. 266-71, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Zakrzewicz, Kutlimuratov, Atanasova, Zakrzewicz, Reichert, Schmitz, Wilhelm, Lochnit, Padberg, Grau: "Attenuation of acute rat renal allograft rejection by apolipoprotein E-mimetic Peptide." in: Transplantation, Vol. 99, Issue 5, pp. 925-34, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting