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Details for Product No. ABIN152561

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody

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G3PD, GAPD, Gapd, cb609, gapd, mg:bb02e05, wu:fb33a10, wu:ft80f05, KNC-NDS6, G3PDH, CG8893, Dmel\\CG8893, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh, Gapdh-2, Gapdh13F, BEST:GH12586, CG12055,  ... show more
»Alternatives Bird (Avian)
»Alternatives Mouse
Clonality Polyclonal
»Alternatives Un-conjugated
»Alternatives Immunofluorescence (IF), Western Blotting (WB)
Pubmed 13 references available
Catalog no. ABIN152561
Quantity 0.5 mL
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Immunogen The immunogen used to raise this particular antibody was extensively purified pigGAPDH.
Isotype IgG1
Specificity Species Reactivity: ABIN152561 reacts with GAPDH from human, cow, pig, mouse, rat and bird. SinceGAPDH is one of the most conserved proteins known, it is likely that the antibody is effective on other species also.
Cross-Reactivity Bird (Avian), Mammalian
Purification Supernatant
Alternative Name GAPDH
Background GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible forcatalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation ofglyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amountsand which is required at all times for an important house keeping functions, levels ofGAPDH mRNA are often used as standards in studies of mRNA expression.Increasingly, scientists are making use of specific antibodies to GAPDH as loadingcontrols for western blotting experiments. Apart from a role in glycolysis, GAPDH mayhave other roles such as in the activation of transcription (1). GAPDH is reported to bindto a variety of other proteins, including the amyloid precursor protein, mutations in whichcause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin,the protein product aberrant forms of which are causative of Huntington's disease (2,3).Associations with actin and tubulin have also be reported. The protein may also have arole in the regulation of apoptosis, and interestingly migrates from the cytoplasm into thenucleus when cells become apoptotic (4). Alternate Names: anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody,anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685antibody.
Gene ID 2597, 14433, 24383
Research Area Cancer, Metabolism, Alzheimer's Disease, Enzymes
Application Notes Suggested working dilutions: immunofluorescence 1:100,Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/ml
Buffer Preservative: 10 mM Sodium Azide.
Preservative Sodium azide
Storage 4 °C
Akassoglou, Malester, Xu et al.: "Brain-specific deletion of neuropathy target esterase/swisscheese results in neurodegeneration." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 14, pp. 5075-80, 2004 (PubMed).

Jia, Takimoto: "Mitogen-activated protein kinases control cardiac KChIP2 gene expression." in: Circulation research, Vol. 98, Issue 3, pp. 386-93, 2006 (PubMed).

Pisitkun, Bieniek, Tchapyjnikov et al.: "High-throughput identification of IMCD proteins using LC-MS/MS." in: Physiological genomics, Vol. 25, Issue 2, pp. 263-76, 2006 (PubMed).

Arthur, Georgi, Akassoglou et al.: "Inhibition of apoptosis by P2Y2 receptor activation: novel pathways for neuronal survival." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 14, pp. 3798-804, 2006 (PubMed).

Azfer, Niu, Rogers et al.: "Activation of endoplasmic reticulum stress response during the development of ischemic heart disease." in: American journal of physiology. Heart and circulatory physiology, Vol. 291, Issue 3, pp. H1411-20, 2006 (PubMed).

Barry, Davidsen, Limesand et al.: "Developmental changes in ovine myocardial glucose transporters and insulin signaling following hyperthermia-induced intrauterine fetal growth restriction." in: Experimental biology and medicine (Maywood, N.J.), Vol. 231, Issue 5, pp. 566-75, 2006 (PubMed).

Le Boeuf, Houle, Sussman et al.: "Phosphorylation of focal adhesion kinase (FAK) on Ser732 is induced by rho-dependent kinase and is essential for proline-rich tyrosine kinase-2-mediated phosphorylation of FAK on Tyr407 in response to vascular endothelial growth factor." in: Molecular biology of the cell, Vol. 17, Issue 8, pp. 3508-20, 2006 (PubMed).

Shibata, Gabbard, Yamashita et al.: "Heat-killed BCG induces biphasic cyclooxygenase 2+ splenic macrophage formation--role of IL-10 and bone marrow precursors." in: Journal of leukocyte biology, Vol. 80, Issue 3, pp. 590-8, 2006 (PubMed).

Kanadia, Shin, Yuan et al.: "Reversal of RNA missplicing and myotonia after muscleblind overexpression in a mouse poly(CUG) model for myotonic dystrophy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 31, pp. 11748-53, 2006 (PubMed).

Fatma, Yakubov, Anwar et al.: "Pluronic L81 enhances triacylglycerol accumulation in the cytosol and inhibits chylomicron secretion." in: Journal of lipid research, Vol. 47, Issue 11, pp. 2422-32, 2006 (PubMed).

Block, Gorin, Hoover et al.: "NAD(P)H oxidases regulate HIF-2alpha protein expression." in: The Journal of biological chemistry, Vol. 282, Issue 11, pp. 8019-26, 2007 (PubMed).

Redman, He, Hartnett et al.: "Apoptotic surge of potassium currents is mediated by p38 phosphorylation of Kv2.1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 9, pp. 3568-73, 2007 (PubMed).

Porchia, Guerra, Wang et al.: "2-amino-N-{4-[5-(2-phenanthrenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]-phenyl} acetamide (OSU-03012), a celecoxib derivative, directly targets p21-activated kinase." in: Molecular pharmacology, Vol. 72, Issue 5, pp. 1124-31, 2007 (PubMed).

Alternatives for antigen "Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)", type "Antibodies"
Hosts (251), (199), (37), (22), (19)
Reactivities (396), (246), (211), (108), (59), (39), (38), (36), (27), (26), (24), (20), (16), (14), (12), (11), (10), (9), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (446), (238), (139), (81), (79), (60), (50), (37), (17), (13), (7), (6), (5), (5), (4), (2), (2), (1), (1)
Conjugates (30), (24), (7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1)
Epitopes (79), (36), (13), (10), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)