Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody

Antigen: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

Synonyms: G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Clonality: Polyclonal

Host: Mouse

Reactivity: Bird (Avian)

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN152561

Additional Information

Alternative name: GAPDH

Gene ID: 2597, 14433, 24383

Immunogen: The immunogen used to raise this particular antibody was extensively purified pigGAPDH.

Cross-Reactivity: Bird (Avian), Mammalian

Format: Supernatant

Isotype: IgG1

Description: GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible forcatalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation ofglyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amountsand which is required at all times for an important house keeping functions, levels ofGAPDH mRNA are often used as standards in studies of mRNA expression.Increasingly, scientists are making use of specific antibodies to GAPDH as loadingcontrols for western blotting experiments. Apart from a role in glycolysis, GAPDH mayhave other roles such as in the activation of transcription (1). GAPDH is reported to bindto a variety of other proteins, including the amyloid precursor protein, mutations in whichcause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin,the protein product aberrant forms of which are causative of Huntington's disease (2,3).Associations with actin and tubulin have also be reported. The protein may also have arole in the regulation of apoptosis, and interestingly migrates from the cytoplasm into thenucleus when cells become apoptotic (4). Alternate Names: anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody,anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685antibody.

Specificity: Species Reactivity: ABIN152561 reacts with GAPDH from human, cow, pig, mouse, rat and bird. SinceGAPDH is one of the most conserved proteins known, it is likely that the antibody is effective on other species also.

Synonyms: G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Application Details

Application Notes: Suggested working dilutions: immunofluorescence 1:100,Western Blot 1:1000

Concentration: 1 mg/ml

Purification: Supernatant

Buffer: Preservative: 10 mM Sodium Azide.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Research Area: Cancer, Metabolism, Alzheimer's Disease, Enzymes

Restrictions: For Research Use only

Images

Sciatic nerves of mouse wild type (+/+), heterozygous (+/o) and homozygous (o/o) for knock out of peripheral myelin protein 21 (pmp21) were homogenized in SDS-PAGE sample buffer and run out for western blots. Blots were probed with ABIN152561 mouse monoclonal antibody to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Antibody was used at dilution of 1:1,000. Signal was revealed in a few seconds with chemiluminescence, indicating that lower antibody concentrations would also have worked well. Note the sharp clear band at 38kDa, the expected molecular weight for GAPDH.

Human neuroblastma line Sh-SY5Y stained with anti-GAPDH (ABIN152561) [green] and an anti-NF-H [red], counterstained with a fluorescent DNA probe [blue].

Publications

Akassoglou, Malester, Xu et al.: "Brain-specific deletion of neuropathy target esterase/swisscheese results in neurodegeneration." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 14, pp. 5075-80, 2004 (PubMed).

Jia, Takimoto: "Mitogen-activated protein kinases control cardiac KChIP2 gene expression." in: Circulation research, Vol. 98, Issue 3, pp. 386-93, 2006 (PubMed).

Pisitkun, Bieniek, Tchapyjnikov et al.: "High-throughput identification of IMCD proteins using LC-MS/MS." in: Physiological genomics, Vol. 25, Issue 2, pp. 263-76, 2006 (PubMed).

Arthur, Georgi, Akassoglou et al.: "Inhibition of apoptosis by P2Y2 receptor activation: novel pathways for neuronal survival." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 14, pp. 3798-804, 2006 (PubMed).

Azfer, Niu, Rogers et al.: "Activation of endoplasmic reticulum stress response during the development of ischemic heart disease." in: American journal of physiology. Heart and circulatory physiology, Vol. 291, Issue 3, pp. H1411-20, 2006 (PubMed).

Barry, Davidsen, Limesand et al.: "Developmental changes in ovine myocardial glucose transporters and insulin signaling following hyperthermia-induced intrauterine fetal growth restriction." in: Experimental biology and medicine (Maywood, N.J.), Vol. 231, Issue 5, pp. 566-75, 2006 (PubMed).

Le Boeuf, Houle, Sussman et al.: "Phosphorylation of focal adhesion kinase (FAK) on Ser732 is induced by rho-dependent kinase and is essential for proline-rich tyrosine kinase-2-mediated phosphorylation of FAK on Tyr407 in response to vascular endothelial growth factor." in: Molecular biology of the cell, Vol. 17, Issue 8, pp. 3508-20, 2006 (PubMed).

Shibata, Gabbard, Yamashita et al.: "Heat-killed BCG induces biphasic cyclooxygenase 2+ splenic macrophage formation--role of IL-10 and bone marrow precursors." in: Journal of leukocyte biology, Vol. 80, Issue 3, pp. 590-8, 2006 (PubMed).

Kanadia, Shin, Yuan et al.: "Reversal of RNA missplicing and myotonia after muscleblind overexpression in a mouse poly(CUG) model for myotonic dystrophy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 31, pp. 11748-53, 2006 (PubMed).

Fatma, Yakubov, Anwar et al.: "Pluronic L81 enhances triacylglycerol accumulation in the cytosol and inhibits chylomicron secretion." in: Journal of lipid research, Vol. 47, Issue 11, pp. 2422-32, 2006 (PubMed).

Block, Gorin, Hoover et al.: "NAD(P)H oxidases regulate HIF-2alpha protein expression." in: The Journal of biological chemistry, Vol. 282, Issue 11, pp. 8019-26, 2007 (PubMed).

Redman, He, Hartnett et al.: "Apoptotic surge of potassium currents is mediated by p38 phosphorylation of Kv2.1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 9, pp. 3568-73, 2007 (PubMed).

Porchia, Guerra, Wang et al.: "2-amino-N-{4-[5-(2-phenanthrenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]-phenyl} acetamide (OSU-03012), a celecoxib derivative, directly targets p21-activated kinase." in: Molecular pharmacology, Vol. 72, Issue 5, pp. 1124-31, 2007 (PubMed).