Vimentin (VIM) antibody

Details for Product No. ABIN152563
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Antigen
Synonyms CTRCT30, cb28, VIM, Vimentin, vim, vime
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(443), (166), (154), (85), (78), (54), (44), (18), (12), (11), (10), (9), (6), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Host
Chicken
(229), (229), (25), (9), (7), (5), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(17), (15), (9), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(364), (161), (145), (144), (90), (87), (48), (44), (40), (37), (6), (3), (3), (3), (3), (2), (2), (1), (1)
Pubmed 2 references available
Catalog no. ABIN152563
Quantity 0.1 mL
Price
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Immunogen The immunogen used to generate our antibody was recombinant human vimentinpurified from E. coli. [Swiss-prot# P08670].
Specificity Species Reactivity: Human, mouse, and rat. Theoretical Molecular Weight: 53.6 kDa.
Alternative Name Vimentin
Background Vimentin is the 10nm or intermediate filament protein subunit found in many kinds ofmesenchymal and epithelia cells, in many kinds of cells in tissue culture, and indeveloping neuronal and astrocytic precursor cells in the central nervous system.Vimentin frequently forms co-polymers with other intermediate filament proteins, such asGFAP (in astrocytes), with desmin (in muscle cells) and neurofilament proteins (indeveloping neurons). Antibodies to vimentin are useful in studies of stem cells and aregenerally used to reveal the filamentous cytoskeleton. Alternate Names: anti-FLJ36605 antibody, anti-VIM antibody.
Gene ID 7431, 22352, 81818
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Application Notes Suggested working dilutions: immunofluorescence 1:5000,Immunohistochemistry-Paraffin 1:150
Restrictions For Research Use only
Format Liquid
Buffer Preservative: 5 mM sodium azide.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-Vimentin (VIM) antibody View of mixed neuron/glial cultures stained with ABIN152563 (green) and rabbit antibody to GFAP antibody ABIN152487(red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.
anti-Vimentin (VIM) antibody (2) Western blot of crude extract of human embryonic kidney Hek293 cells stained with ABIN152563, showing a single strong clean band at ~50kDa.
General Rankin, Tomaszewski, Haase: "Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor." in: Cancer research, Vol. 66, Issue 5, pp. 2576-83, 2006 (PubMed).

Johnstone, Mongroo, Rich et al.: "Parvin-beta inhibits breast cancer tumorigenicity and promotes CDK9-mediated peroxisome proliferator-activated receptor gamma 1 phosphorylation." in: Molecular and cellular biology, Vol. 28, Issue 2, pp. 687-704, 2008 (PubMed).

Hosts (229), (229), (25), (9), (7), (5), (4)
Reactivities (443), (166), (154), (85), (78), (54), (44), (18), (12), (11), (10), (9), (6), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1)
Applications (364), (161), (145), (144), (90), (87), (48), (44), (40), (37), (6), (3), (3), (3), (3), (2), (2), (1), (1)
Conjugates (17), (15), (9), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (1), (1)
Epitopes (20), (18), (16), (15), (8), (8), (6), (5), (5), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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