Vimentin (VIM) antibody

Antigen: Vimentin (VIM)

Synonyms: FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime

Clonality: Polyclonal

Host: Chicken

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN152563

Additional Information

Alternative name: Vimentin

Gene ID: 7431, 22352, 81818

UniProt: P08670

Immunogen: The immunogen used to generate our antibody was recombinant human vimentinpurified from E. coli. [Swiss-prot# P08670].

Description: Vimentin is the 10nm or intermediate filament protein subunit found in many kinds ofmesenchymal and epithelia cells, in many kinds of cells in tissue culture, and indeveloping neuronal and astrocytic precursor cells in the central nervous system.Vimentin frequently forms co-polymers with other intermediate filament proteins, such asGFAP (in astrocytes), with desmin (in muscle cells) and neurofilament proteins (indeveloping neurons). Antibodies to vimentin are useful in studies of stem cells and aregenerally used to reveal the filamentous cytoskeleton. Alternate Names: anti-FLJ36605 antibody, anti-VIM antibody.

Specificity: Species Reactivity: Human, mouse, and rat. Theoretical Molecular Weight: 53.6 kDa.

Application Details

Application Notes: Suggested working dilutions: immunofluorescence 1:5000,Immunohistochemistry-Paraffin 1:150

Buffer: Preservative: 5 mM sodium azide.

Storage: Store at 4C for short term. Aliquot and store at -20C for long term. Avoid freeze-thawcycles.

Research Area: Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers

Restrictions: For Research Use only

Images

View of mixed neuron/glial cultures stained with ABIN152563 (green) and rabbit antibody to GFAP antibody ABIN152487(red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two protein assemble to produce heteropolymer filaments.

Western blot of crude extract of human embryonic kidney Hek293 cells stained with ABIN152563, showing a single strong clean band at ~50kDa.

Publications

Rankin, Tomaszewski, Haase: "Renal cyst development in mice with conditional inactivation of the von Hippel-Lindau tumor suppressor." in: Cancer research, Vol. 66, Issue 5, pp. 2576-83, 2006 (PubMed).

Johnstone, Mongroo, Rich et al.: "Parvin-beta inhibits breast cancer tumorigenicity and promotes CDK9-mediated peroxisome proliferator-activated receptor gamma 1 phosphorylation." in: Molecular and cellular biology, Vol. 28, Issue 2, pp. 687-704, 2008 (PubMed).