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Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (Thr75) antibody

Details for Product No. ABIN152574
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Antigen
Synonyms darpp32, darpp-32, Darpp-32, Darpp32, DARPP-32, DARPP32, AU040756
Epitope
Thr75
(42), (31), (10), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(132), (114), (103), (51), (49), (36), (36), (12), (4), (4), (4), (1), (1), (1)
Host
Rabbit
(138), (8), (2), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB)
(113), (55), (46), (30), (22), (18), (6), (4), (2), (2), (2), (1)
Pubmed 4 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN152574
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Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 conjugated to KLH
Specificity Specific for the ~32k DARPP-32 protein phosphorylated at Thr75. Immunolabeling is blocked by lambda-phosphatase treatment.
Purification Affinity purified
Alternative Name DARPP32
Background DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associatedwith dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The proteininhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, whenDARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation ofdopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thoughtto play important roles in the actions of alcohol, caffeine and Prozac. Alternate Names: anti-DARPP 32 antibody, anti-DARPP32 antibody, anti-Dopamine- and cAMP-regulatedneuronal phosphoprotein antibody.
Gene Symbol: PPP1R1B
Gene ID 84152, 19049, 360616
Application Notes This antibody is useful for Western Blot 1:1000. The investigator should determine the optimal working dilution for a specific application.
Recommended dilutions: Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES [pH 7.5] contains 150 mM NaCl, 0.1 mg/ml BSA and 50 % glycerol
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (Thr75) antibody Immunolabeling of rat caudate lysates prepared from caudate slices that had been incubated in the absence and presence of okadaic acid. The labeling by the antibody to DARPP-32 Thr75 is markedly increased by the okadaic acid treatment.
General Maldve, Zhang, Ferrani-Kile et al.: "DARPP-32 and regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens." in: Nature neuroscience, Vol. 5, Issue 7, pp. 641-8, 2002 (PubMed).

Lindskog, Svenningsson, Pozzi et al.: "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine." in: Nature, Vol. 418, Issue 6899, pp. 774-8, 2002 (PubMed).

Svenningsson, Tzavara, Carruthers et al.: "Diverse psychotomimetics act through a common signaling pathway." in: Science (New York, N.Y.), Vol. 302, Issue 5649, pp. 1412-5, 2003 (PubMed).

Han, Han, Tiwari et al.: "Spatial targeting of type II protein kinase A to filopodia mediates the regulation of growth cone guidance by cAMP." in: The Journal of cell biology, Vol. 176, Issue 1, pp. 101-11, 2007 (PubMed).

Validation Images
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