Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (Thr75) antibody

Details for Product No. ABIN152574
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms darpp32, darpp-32, Darpp-32, Darpp32, DARPP-32, DARPP32, AU040756
(50), (34), (16), (12), (7), (6), (4), (4), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Rat (Rattus)
(165), (132), (115), (19), (19), (18), (7), (6), (6), (3), (2), (1)
(173), (13), (3), (2), (1)
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Western Blotting (WB)
(143), (62), (62), (35), (30), (19), (5), (5), (4), (2), (2)
Pubmed 4 references available
Quantity 0.1 mL
Shipping to United States ( )
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 conjugated to KLH
Specificity Specific for the ~32k DARPP-32 protein phosphorylated at Thr75. Immunolabeling is blocked by lambda-phosphatase treatment.
Purification Affinity purified
Alternative Name DARPP32 (PPP1R1B Antibody Abstract)
Background DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associatedwith dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The proteininhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, whenDARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation ofdopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thoughtto play important roles in the actions of alcohol, caffeine and Prozac. Alternate Names: anti-DARPP 32 antibody, anti-DARPP32 antibody, anti-Dopamine- and cAMP-regulatedneuronal phosphoprotein antibody.
Gene Symbol: PPP1R1B
Gene ID 84152, 19049, 360616
Application Notes This antibody is useful for Western Blot 1:1000. The investigator should determine the optimal working dilution for a specific application.
Recommended dilutions: Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES [pH 7.5] contains 150 mM NaCl, 0.1 mg/ml BSA and 50 % glycerol
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (Thr75) antibody Immunolabeling of rat caudate lysates prepared from caudate slices that had been incu...
General Han, Han, Tiwari et al.: "Spatial targeting of type II protein kinase A to filopodia mediates the regulation of growth cone guidance by cAMP." in: The Journal of cell biology, Vol. 176, Issue 1, pp. 101-11, 2007 (PubMed).

Svenningsson, Tzavara, Carruthers et al.: "Diverse psychotomimetics act through a common signaling pathway." in: Science (New York, N.Y.), Vol. 302, Issue 5649, pp. 1412-5, 2003 (PubMed).

Lindskog, Svenningsson, Pozzi et al.: "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine." in: Nature, Vol. 418, Issue 6899, pp. 774-8, 2002 (PubMed).

Maldve, Zhang, Ferrani-Kile et al.: "DARPP-32 and regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens." in: Nature neuroscience, Vol. 5, Issue 7, pp. 641-8, 2002 (PubMed).

Validation Images
Catalog No. ABIN152574
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)
Did you look for something else?