Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pSer75) antibody

Antigen: Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B)

Synonyms: Darpp32, AU040756, DARPP-32, DARPP32, FLJ20940, Darpp-32, darpp32, darpp-32, PPP1R1B, MGC139723

Binding Site: pSer75

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN152574

Additional Information

Alternative name: DARPP32

Gene ID: 84152, 19049, 360616

Immunogen: Synthetic phosphopeptide corresponding to amino acids residues surrounding thephospho Thr75 of DARPP-32. The sequence of the immunogen is identical in humanand mouse.

Cross-Reactivity: Human, Mouse (Murine)

Description: DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associatedwith dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The proteininhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, whenDARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation ofdopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thoughtto play important roles in the actions of alcohol, caffeine and Prozac. Alternate Names: anti-DARPP 32 antibody, anti-DARPP32 antibody, anti-Dopamine- and cAMP-regulatedneuronal phosphoprotein antibody.

Specificity: Specific for the ~34k DARPP-32 phosphorylated at Thr75. Phosphorylated:Thr75. Species Reactivity: Reacts with human, mouse, rat, bovine, canine, chicken, and primate. Localization: Cytoplasmic.

Application Details

Application Notes: Suggested working dilutions: Western Blot 1:1000

Purification: Aff - Purified

Buffer: 0.1 ml affinity purified antibody in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mlBSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blot.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

Immunolabeling of rat caudate lysates prepared from caudate slices that had been incubated in the absence and presence of okadaic acid. The labeling by the antibody to DARPP-32 Thr75 is markedly increased by the okadaic acid treatment.

Publications

Maldve, Zhang, Ferrani-Kile et al.: "DARPP-32 and regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens." in: Nature neuroscience, Vol. 5, Issue 7, pp. 641-8, 2002 (PubMed).

Lindskog, Svenningsson, Pozzi et al.: "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine." in: Nature, Vol. 418, Issue 6899, pp. 774-8, 2002 (PubMed).

Svenningsson, Tzavara, Carruthers et al.: "Diverse psychotomimetics act through a common signaling pathway." in: Science (New York, N.Y.), Vol. 302, Issue 5649, pp. 1412-5, 2003 (PubMed).

Han, Han, Tiwari et al.: "Spatial targeting of type II protein kinase A to filopodia mediates the regulation of growth cone guidance by cAMP." in: The Journal of cell biology, Vol. 176, Issue 1, pp. 101-11, 2007 (PubMed).