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Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (Thr75) antibody

Details for Product No. ABIN152574, Supplier: Log in to see
Antigen
  • AU040756
  • DARPP-32
  • Darpp-32
  • darpp-32
  • DARPP32
  • Darpp32
  • darpp32
Epitope
Thr75
41
39
19
13
7
6
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
157
117
107
21
19
18
7
6
6
4
3
2
Host
Rabbit
161
15
3
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
139
61
51
32
23
21
7
6
3
2
1
Supplier
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Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 conjugated to KLH
Specificity Specific for the ~32k DARPP-32 protein phosphorylated at Thr75. Immunolabeling is blocked by lambda-phosphatase treatment.
Purification Affinity purified
Alternative Name DARPP-32 (PPP1R1B Antibody Abstract)
Background DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associatedwith dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The proteininhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, whenDARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation ofdopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thoughtto play important roles in the actions of alcohol, caffeine and Prozac. Alternate Names: anti-DARPP 32 antibody, anti-DARPP32 antibody, anti-Dopamine- and cAMP-regulatedneuronal phosphoprotein antibody.
Gene Symbol: PPP1R1B
Gene ID 84152, 19049, 360616
Application Notes This antibody is useful for Western Blot 1:1000. The investigator should determine the optimal working dilution for a specific application.
Recommended dilutions: Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES [pH 7.5] contains 150 mM NaCl, 0.1 mg/ml BSA and 50 % glycerol
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (Thr75) antibody (ABIN152574) Immunolabeling of rat caudate lysates prepared from caudate slices that had been incu...
Product cited in: Cunha, Matheus, Moretti, Sampaio, Poli, Santos, Colle, Cunha, Blum-Silva, Sandjo, Reginatto, Rodrigues, Farina, Prediger: "Agmatine attenuates reserpine-induced oral dyskinesia in mice: Role of oxidative stress, nitric oxide and glutamate NMDA receptors." in: Behavioural brain research, Vol. 312, pp. 64-76, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Hamel, Bouchard, Ferrario, Hassan, Aguilar-Mahecha, Buchanan, Quenneville, Miller, Basik: "Both t-Darpp and DARPP-32 can cause resistance to trastuzumab in breast cancer cells and are frequently expressed in primary breast cancers." in: Breast cancer research and treatment, Vol. 120, Issue 1, pp. 47-57, 2010 (PubMed). (Sample species: Human). Further details: Western Blotting

Han, Han, Tiwari, Wen, Zheng: "Spatial targeting of type II protein kinase A to filopodia mediates the regulation of growth cone guidance by cAMP." in: The Journal of cell biology, Vol. 176, Issue 1, pp. 101-11, 2007 (PubMed).

Svenningsson, Tzavara, Carruthers, Rachleff, Wattler, Nehls, McKinzie, Fienberg, Nomikos, Greengard: "Diverse psychotomimetics act through a common signaling pathway." in: Science (New York, N.Y.), Vol. 302, Issue 5649, pp. 1412-5, 2003 (PubMed).

Maldve, Zhang, Ferrani-Kile, Schreiber, Lippmann, Snyder, Fienberg, Leslie, Gonzales, Morrisett: "DARPP-32 and regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens." in: Nature neuroscience, Vol. 5, Issue 7, pp. 641-8, 2002 (PubMed).

Lindskog, Svenningsson, Pozzi, Kim, Fienberg, Bibb, Fredholm, Nairn, Greengard, Fisone: "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine." in: Nature, Vol. 418, Issue 6899, pp. 774-8, 2002 (PubMed).

Greengard: "The neurobiology of slow synaptic transmission." in: Science (New York, N.Y.), Vol. 294, Issue 5544, pp. 1024-30, 2001 (PubMed).

Bibb, Snyder, Nishi, Yan, Meijer, Fienberg, Tsai, Kwon, Girault, Czernik, Huganir, Hemmings, Nairn, Greengard: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, 2000 (PubMed).

Fienberg, Hiroi, Mermelstein, Song, Snyder, Nishi, Cheramy, OCallaghan, Miller, Cole, Corbett, Haile, Cooper, Onn, Grace, Ouimet, White, Hyman, Surmeier, Girault, Nestler, Greengard: "DARPP-32: regulator of the efficacy of dopaminergic neurotransmission." in: Science (New York, N.Y.), Vol. 281, Issue 5378, pp. 838-42, 1998 (PubMed).

Ouimet, Miller, Hemmings, Walaas, Greengard: "DARPP-32, a dopamine- and adenosine 3':5'-monophosphate-regulated phosphoprotein enriched in dopamine-innervated brain regions. III. Immunocytochemical localization." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 4, Issue 1, pp. 111-24, 1984 (PubMed).