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Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr75) antibody

Details for Product No. ABIN152574, Supplier: Log in to see
Antigen
  • darpp32
  • darpp-32
  • Darpp-32
  • Darpp32
  • DARPP-32
  • DARPP32
  • AU040756
  • protein phosphatase 1, regulatory (inhibitor) subunit 1B
  • protein phosphatase 1 regulatory subunit 1B-like
  • ppp1r1b
  • PPP1R1B
  • LOC100355399
  • LOC100736966
  • Ppp1r1b
Epitope
pThr75
41
39
20
13
8
7
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
161
109
107
21
19
18
7
6
6
4
3
2
Host
Rabbit
163
17
3
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
142
62
53
32
23
22
7
7
3
2
1
1
Options
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Available images

Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phosphoThr75 of rat DARPP-32.
Specificity Specific for the ~32k DARPP-32 protein phosphorylated at Thr75. Immunolabeling is blocked by lambda-phosphatase treatment.
Purification Immunogen affinity purified
Alternative Name DARPP-32 (PPP1R1B Antibody Abstract)
Background Gene Symbol: PPP1R1B
Molecular Weight Theoretical MW: 32 kDa
Gene ID 84152
UniProt Q6J4I0
Application Notes Western Blot 1:1000The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 0.15M NaCl, 0.1 mg/mL BSA and 50 % Glycerol
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr75) antibody (ABIN152574) Immunolabeling of rat caudate lysates prepared from caudate slices that had been incu...
Western Blotting (WB) image for anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr75) antibody (ABIN152574) Western Blot: DARPP32 [p Thr75] Antibody [ABIN152574] - Analysis of rat caudate lysat...
Product cited in: Cunha, Matheus, Moretti, Sampaio, Poli, Santos, Colle, Cunha, Blum-Silva, Sandjo, Reginatto, Rodrigues, Farina, Prediger: "Agmatine attenuates reserpine-induced oral dyskinesia in mice: Role of oxidative stress, nitric oxide and glutamate NMDA receptors." in: Behavioural brain research, Vol. 312, pp. 64-76, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Hamel, Bouchard, Ferrario, Hassan, Aguilar-Mahecha, Buchanan, Quenneville, Miller, Basik: "Both t-Darpp and DARPP-32 can cause resistance to trastuzumab in breast cancer cells and are frequently expressed in primary breast cancers." in: Breast cancer research and treatment, Vol. 120, Issue 1, pp. 47-57, 2010 (PubMed). (Sample species: Human). Further details: Western Blotting

Han, Han, Tiwari, Wen, Zheng: "Spatial targeting of type II protein kinase A to filopodia mediates the regulation of growth cone guidance by cAMP." in: The Journal of cell biology, Vol. 176, Issue 1, pp. 101-11, 2007 (PubMed).

Svenningsson, Tzavara, Carruthers, Rachleff, Wattler, Nehls, McKinzie, Fienberg, Nomikos, Greengard: "Diverse psychotomimetics act through a common signaling pathway." in: Science (New York, N.Y.), Vol. 302, Issue 5649, pp. 1412-5, 2003 (PubMed).

Maldve, Zhang, Ferrani-Kile, Schreiber, Lippmann, Snyder, Fienberg, Leslie, Gonzales, Morrisett: "DARPP-32 and regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens." in: Nature neuroscience, Vol. 5, Issue 7, pp. 641-8, 2002 (PubMed).

Lindskog, Svenningsson, Pozzi, Kim, Fienberg, Bibb, Fredholm, Nairn, Greengard, Fisone: "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine." in: Nature, Vol. 418, Issue 6899, pp. 774-8, 2002 (PubMed).

Greengard: "The neurobiology of slow synaptic transmission." in: Science (New York, N.Y.), Vol. 294, Issue 5544, pp. 1024-30, 2001 (PubMed).

Bibb, Snyder, Nishi, Yan, Meijer, Fienberg, Tsai, Kwon, Girault, Czernik, Huganir, Hemmings, Nairn, Greengard: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, 2000 (PubMed).

Fienberg, Hiroi, Mermelstein, Song, Snyder, Nishi, Cheramy, OCallaghan, Miller, Cole, Corbett, Haile, Cooper, Onn, Grace, Ouimet, White, Hyman, Surmeier, Girault, Nestler, Greengard: "DARPP-32: regulator of the efficacy of dopaminergic neurotransmission." in: Science (New York, N.Y.), Vol. 281, Issue 5378, pp. 838-42, 1998 (PubMed).

Ouimet, Miller, Hemmings, Walaas, Greengard: "DARPP-32, a dopamine- and adenosine 3':5'-monophosphate-regulated phosphoprotein enriched in dopamine-innervated brain regions. III. Immunocytochemical localization." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 4, Issue 1, pp. 111-24, 1984 (PubMed).