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Notch1 antibody (Notch 1) (cleaved)

Details for Product anti-NOTCH1 Antibody No. ABIN152590, Supplier: Login to see New
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Synonyms notch1, NOTCH1, hn1, tan1, notch, xotch, xnotch, notch-1, xnotch1, TAN1, hN1, 9930111A19Rik, Mis6, N1, Tan1, lin-12, NOTCH, Xotch, notch1-a
cleaved, N-Term
(50), (37), (36), (17), (17), (10), (10), (7), (7), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus)
(213), (161), (62), (6), (2), (2), (2), (1), (1), (1)
(145), (139), (15), (8), (7), (2), (1)
This Notch1 antibody is un-conjugated
(26), (15), (13), (7), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Western Blotting (WB), ELISA
(190), (100), (79), (79), (52), (37), (36), (16), (15), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Quantity 0.2 mL
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Immunogen A synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
Specificity Notch-1 does not react with mouse N2, N3, or N4. It only recognizes the cleaved intracellular (activated) form (NICD).
Purification Whole antisera
Alternative Name NOTCH1 (NOTCH1 Antibody Abstract)
Background Notch is synthesized in the endoplasmic reticulum as an inactive form which isproteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi networkbefore it reaches the plasma membrane to yield an active, ligand-accessible form.Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC).Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme(TACE) to yield a membrane-associated intermediate fragment called Notchextracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependentgamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from themembrane which then translocates to the nucleus to activate transcription ofdownstream genes. Alternate Names: anti-Notch1 antibody, anti-Notch-1 antibody, anti-TAN1 antibody, anti-TranslocationAssociated Notch Homolog antibody, anti-Neurogenic locus Notch homolog protein 1antibody, anti-hN1 antibody.
Gene Symbol: NOTCH1
Gene ID 4851, 18128, 25496
Research Area Receptors, Signaling, Inflammation, Immunology
Pathways Notch Signaling
Application Notes This antibody is reactive with Notch 1 by western blotting. A strong response was also detected by ELISA against the immunizing peptide.This product was assayed by immunoblot against recombinant mouse Notch constructs in 293 cells. An 80 kDa band corresponding - Notch 1 was observed at a 1:500 dilution. This product was also assayed against the peptide immunogen in a standard capture ELISA using Peroxidase conjugated anti-Rabbit IgG [Goat] and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 - 1:60,000 is suggested for this product.
Recommended dilutions: ELISA 1:20000-1:60000, Western Blot 1:500-1:2000
Restrictions For Research Use only
Format Liquid
Buffer Whole antisera diluted in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 (total protein concentration is 80 mg/ml by Refractometry), Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Western Blotting (WB) image for anti-Notch1 antibody (Notch 1) (cleaved) (ABIN152590) Rabbit polyclonal anti-Notch 1 (ABIN152590) at 1:500, against myc-tagged transiently ...
Western Blotting (WB) image for anti-Notch1 antibody (Notch 1) (cleaved) (ABIN152590) anti-Notch 1 (NOTCH1) (N-Term), (cleaved) antibody (Image 2)
Product cited in: Milano, McKay, Dagenais et al.: "Modulation of notch processing by gamma-secretase inhibitors causes intestinal goblet cell metaplasia and induction of genes known to specify gut secretory lineage differentiation." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 82, Issue 1, pp. 341-58, 2004 (PubMed).

Background publications Gridley: "Notch signaling and inherited disease syndromes." in: Human molecular genetics, Vol. 12 Spec No 1, pp. R9-13, 2003 (PubMed).

Bertrand, Eckfeldt, Lysholm et al.: "Notch-1 and Notch-2 exhibit unique patterns of expression in human B-lineage cells." in: Leukemia, Vol. 14, Issue 12, pp. 2095-102, 2001 (PubMed).

Catalog No. ABIN152590
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