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SLC7A11 antibody (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11) (N-Term)

Details for Product anti-SLC7A11 Antibody No. ABIN152647, Supplier: Log in to see
Antigen
  • 9930009M05Rik
  • AI451155
  • CCBR1
  • DKFZp468E122
  • SLC7A11
  • sut
  • xCT
Epitope
N-Term, AA 1-50
46
29
16
13
2
1
1
Reactivity
Human
82
34
33
3
3
2
1
1
1
1
1
1
1
1
Host
Rabbit
69
19
Clonality
Polyclonal
Conjugate
This SLC7A11 antibody is un-conjugated
5
5
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
60
32
21
16
15
15
11
9
1
1
Supplier
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Immunogen A synthetic peptide made to a region within the N-terminus of the mouse xCT protein (between residues 1-50). [UniProt# Q9WTR6]
Cross-Reactivity (Details) Predicted to react with mouse,canine based on 100% sequence homology.
Purification Immunogen affinity purified
Alternative Name xCT (SLC7A11 Antibody Abstract)
Background Gene Symbol: SLC7A11
Molecular Weight Theoretical MW: 55 kDa
UniProt Q9WTR6
Application Notes Western Blot 0.5-2 μg/mL, Immunocytochemistry/Immunofluorescence 1:100-1:1000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for xCT Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 50 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS for 1 hour.
. Dilute the rabbit anti-XCT primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.Immunocytochemistry Protocol NB300-317 Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-glycine, 150 mM NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-SLC7A11 antibody (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11) (N-Term) (ABIN152647) anti-Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11) (AA 1-50), (N-Term) antibody
Immunofluorescence (IF) image for anti-SLC7A11 antibody (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11) (N-Term) (ABIN152647) Immunocytochemistry/Immunofluorescence: xCT Antibody [ABIN152647] - xCT antibody was ...
Product cited in: Linher-Melville, Haftchenary, Gunning, Singh: "Signal transducer and activator of transcription 3 and 5 regulate system Xc- and redox balance in human breast cancer cells." in: Molecular and cellular biochemistry, Vol. 405, Issue 1-2, pp. 205-21, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Pampliega, Domercq, Soria, Villoslada, Rodríguez-Antigüedad, Matute: "Increased expression of cystine/glutamate antiporter in multiple sclerosis." in: Journal of neuroinflammation, Vol. 8, pp. 63, 2011 (PubMed). Further details: Immunohistochemistry (Frozen Sections)

Liu, Blower, Pham, Fang, Dai, Wise, Green, Teitel, Ning, Ling, Lyn-Cook, Kadlubar, Sadée, Huang: "Cystine-glutamate transporter SLC7A11 mediates resistance to geldanamycin but not to 17-(allylamino)-17-demethoxygeldanamycin." in: Molecular pharmacology, Vol. 72, Issue 6, pp. 1637-46, 2007 (PubMed). (Sample species: Human). Further details: Western Blotting

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