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DNMT3B antibody (DNA (Cytosine-5-)-Methyltransferase 3 beta)

Details for Product anti-DNMT3B Antibody No. ABIN152675, Supplier: Log in to see
Antigen
  • cb91
  • cb633
  • DNMT3B
  • dnmt3b
  • dnmt3bl
  • dnmt7
  • ICF
  • ICF1
  • LOC100218113
  • M.HsaIIIB
  • MmuIIIB
  • sb:cb91
  • wu:fb16h07
Reactivity
Human, Mouse (Murine)
112
49
19
2
1
1
1
1
Host
Rabbit
81
32
2
2
1
Clonality
Polyclonal
Conjugate
This DNMT3B antibody is un-conjugated
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
99
56
37
33
32
31
27
22
12
8
3
2
2
2
2
1
1
Supplier
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Immunogen Synthetic peptide: MKGDSRHLNEEEGA-C, corresponding to amino acids 1-14 of Mouse Dnmt3b.
Specificity This detects DNA methyltransferase 3b (Dnmt3b) from human and mouse tissues and cells as well as recombinant human Dnmt3b. This detects, to a lesser extent, full-length human recombinant Dnmt3a.
Purification Immunogen affinity purified
Alternative Name DNMT3B (DNMT3B Antibody Abstract)
Background Gene Symbol: DNMT3B
Gene ID 1789, 13436
UniProt O88509
Application Notes Western Blot 2 μg/mL, Simple Western 20 μg/mL, Chromatin Immunoprecipitation 1:10 - 1:500, Immunohistochemistry 1:100 - 1:1000, Immunohistochemistry-Paraffin 1:100 - 1:1000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS with 1 mg/mL BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DNMT3B antibody (DNA (Cytosine-5-)-Methyltransferase 3 beta) (ABIN152675) Immunohistochemistry-Paraffin: DNMT3B Antibody [ABIN152675] - Analysis showing positi...
Western Blotting (WB) image for anti-DNMT3B antibody (DNA (Cytosine-5-)-Methyltransferase 3 beta) (ABIN152675) Western Blot: DNMT3B Antibody [ABIN152675] - Homogenized mouse brain cells. Image fro...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DNMT3B antibody (DNA (Cytosine-5-)-Methyltransferase 3 beta) (ABIN152675) Immunohistochemistry-Paraffin: DNMT3B Antibody [ABIN152675] - Analysis showing positi...
Simple Western (SimWes) image for anti-DNMT3B antibody (DNA (Cytosine-5-)-Methyltransferase 3 beta) (ABIN152675) Simple Western: DNMT3B Antibody [ABIN152675] - Analysis of 25 ug of Hela cell lysates...
Simple Western (SimWes) image for anti-DNMT3B antibody (DNA (Cytosine-5-)-Methyltransferase 3 beta) (ABIN152675) Simple Western: DNMT3B Antibody [ABIN152675] - Simple Western lane view shows a speci...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DNMT3B antibody (DNA (Cytosine-5-)-Methyltransferase 3 beta) (ABIN152675) Immunohistochemistry-Paraffin: DNMT3B Antibody [ABIN152675] - Analysis showing positi...
Product cited in: Zhong, Men, Lu, Geng, Zhou, Mitsuhashi, Shozu, Maihle, Carmichael, Taylor, Huang: "Metformin alters DNA methylation genome-wide via the H19/SAHH axis." in: Oncogene, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Zhou, Yang, Zhong, Mueller, Men, Zhang, Xie, Giang, Chung, Sun, Lu, Carmichael, Taylor, Huang: "H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase." in: Nature communications, Vol. 6, pp. 10221, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Bhardwaj, Song, Beildeck, Kerkhofs, Castoro, Shanker, De Gendt, Suzuki, Claessens, Issa, Orgebin-Crist, Wilkinson: "DNA demethylation-dependent AR recruitment and GATA factors drive Rhox5 homeobox gene transcription in the epididymis." in: Molecular endocrinology (Baltimore, Md.), Vol. 26, Issue 4, pp. 538-49, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Chromatin Immunoprecipitation

Jin, Tao, Peng, Soo, Wu, Ying, Fields, Delmas, Liu, Qiu, Robertson: "DNA methyltransferase 3B (DNMT3B) mutations in ICF syndrome lead to altered epigenetic modifications and aberrant expression of genes regulating development, neurogenesis and immune function." in: Human molecular genetics, Vol. 17, Issue 5, pp. 690-709, 2008 (PubMed).

Myant, Stancheva: "LSH cooperates with DNA methyltransferases to repress transcription." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 215-26, 2007 (PubMed).

Background publications Okano, Bell, Haber, Li: "DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development." in: Cell, Vol. 99, Issue 3, pp. 247-57, 1999 (PubMed).