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Calnexin (CANX) antibody
Alternatives Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|3 references available|
|Quantity||0.1 ml (1.3 mg/ml)|
|Price||Product not available in this region.|
|Immunogen||Focus human hepatoma cell lysate.|
|Description||Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membraneprotein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecularchaperones and participate in the proper folding of polypeptides and their assembly intomultisubunit proteins. Studies indicate that calnexin associates with the majorhistocompatability complex (MHC) class I heavy chains, partial complexes of the T cellreceptor and B cell membrane immunoglobulin, but not with completed receptorcomplexes. It has been shown that calnexin is a chaperone that retains incompletely orimproperly folded proteins in the ER.The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at thecarboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 andprotein disulfide isomerase. (See catalog #.|
|Specificity||Species Reactivity: Cross-reacts with Human. Not yet tested in other species. Localization: Endoplasmic reticulum.|
|Synonyms||CNX, P90, IP90, FLJ26570, Cnx, AI988026, D11Ertd153e, 1110069N15Rik, DmelCG11958, CG11958, canx, MGC52927, CANX, PP90, zgc:63524, wu:fe06b12, MGC166317, MGC69400, fj49d10, MGC153946, wu:fj24b04, wu:fj49d10, zgc:153946, DKFZp459P042, LOC100283947|
|Application Notes||This antibody can be used in Immunocytochemistry, Immunofluorescence,Immunoprecipitation, and Western Blot. By Western blot, this antibody detects an ~90kDa band representing calnexin in human liver, skin, adrenal gland, kidney, heart, colon,testes and ovary extracts. Immunocytochemical staining of calnexin in Huh-7 cells yieldsa pattern consistent with specific endoplasmic reticulum staining.Recommended Starting Dilutions*:ICC: 1/500WB: 1/4000|
|Buffer||PBS with 0.05% sodium azide. Preservative: 0.05% sodium azide.|
|Storage||Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.|
|Restrictions||For Research Use only|
Galvin, Krishna, Ponchel et al.: "The major histocompatibility complex class I antigen-binding protein p88 is the product of the calnexin gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8452-6, 1992 (PubMed).
Gething, Sambrook: "Protein folding in the cell." in: Nature, Vol. 355, Issue 6355, pp. 33-45, 1992 (PubMed).
Rajagopalan, Xu, Brenner: "Retention of unassembled components of integral membrane proteins by calnexin." in: Science (New York, N.Y.), Vol. 263, Issue 5145, pp. 387-90, 1994 (PubMed).