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Calnexin antibody (CANX)

Details for Product anti-CANX Antibody No. ABIN152677, Supplier: Log in to see
Antigen
  • zgc:63524
  • wu:fe06b12
  • clgn
  • clnx
  • cnx
  • CANX
  • CNX
  • IP90
  • P90
  • canx
  • 1110069N15Rik
  • AI988026
  • Cnx
  • D11Ertd153e
  • PP90
  • CG11958
  • Dmel\CG11958
Alternatives
anti-Human Calnexin antibody for Immunofluorescence
Reactivity
Human, Mouse (Murine)
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60
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37
5
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1
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Host
Mouse
189
28
24
Clonality (Clone)
Monoclonal ()
Conjugate
This Calnexin antibody is un-conjugated
7
7
5
5
5
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4
1
1
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Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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135
106
89
79
69
43
38
11
9
5
3
2
1
1
Supplier
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Immunogen Focus human hepatoma cell lysate.
Clone AF18
Isotype IgG1
Specificity Calnexin (AF18)
Purification Ascites
Alternative Name Calnexin (CANX Antibody Abstract)
Background Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membraneprotein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecularchaperones and participate in the proper folding of polypeptides and their assembly intomultisubunit proteins. Studies indicate that calnexin associates with the majorhistocompatability complex (MHC) class I heavy chains, partial complexes of the T cellreceptor and B cell membrane immunoglobulin, but not with completed receptorcomplexes. It has been shown that calnexin is a chaperone that retains incompletely orimproperly folded proteins in the ER.The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at thecarboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 andprotein disulfide isomerase. (See catalog #.
Gene Symbol: CANX
Gene ID 821
Pathways MAPK Signaling
Application Notes This antibody can be used in Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blot. By Western blot, this antibody detects an ~90 kDa band representing calnexin in human liver, skin, adrenal gland, kidney, heart, colon, testes and ovary extracts. Immunocytochemical staining of calnexin in Huh-7 cells yields a pattern consistent with specific endoplasmic reticulum staining. Recommended Starting Dilutions*: ICC: 1/500 WB: 1/4000 Not tested in other applications. *Optimal dilutions should be determined by the end user.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunoprecipitation 1:10-1:500, Western Blot 1:100-1:2000
Restrictions For Research Use only
Format Liquid
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Background publications Rajagopalan, Xu, Brenner: "Retention of unassembled components of integral membrane proteins by calnexin." in: Science (New York, N.Y.), Vol. 263, Issue 5145, pp. 387-90, 1994 (PubMed).

Gething, Sambrook: "Protein folding in the cell." in: Nature, Vol. 355, Issue 6355, pp. 33-45, 1992 (PubMed).

Galvin, Krishna, Ponchel et al.: "The major histocompatibility complex class I antigen-binding protein p88 is the product of the calnexin gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8452-6, 1992 (PubMed).