Calnexin (CANX) antibody

Details for Product No. ABIN152677
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Antigen
Synonyms CNX, IP90, P90, Cnx, AI988026, D11Ertd153e, 1110069N15Rik, PP90, CG11958, Dmel\\CG11958, cnx, canx, clgn, clnx, zgc:63524, wu:fe06b12, CANX, fj49d10, wu:fj24b04, wu:fj49d10, zgc:153946, DKFZp459P042
Reactivity
Human, Mouse (Murine)
(103), (47), (45), (26), (26), (24), (10), (9), (9), (8), (8), (8), (6), (4), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1)
Host
Mouse
(90), (23), (9)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(113), (49), (39), (29), (25), (25), (21), (10), (10), (8), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN152677
Quantity 0.1 mL
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Immunogen Focus human hepatoma cell lysate.
Clone AF18
Isotype IgG1
Specificity Species Reactivity: Cross-reacts with Human. Not yet tested in other species. Localization: Endoplasmic reticulum.
Cross-Reactivity Human
Purification Ascites
Alternative Name Calnexin
Background Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membraneprotein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecularchaperones and participate in the proper folding of polypeptides and their assembly intomultisubunit proteins. Studies indicate that calnexin associates with the majorhistocompatability complex (MHC) class I heavy chains, partial complexes of the T cellreceptor and B cell membrane immunoglobulin, but not with completed receptorcomplexes. It has been shown that calnexin is a chaperone that retains incompletely orimproperly folded proteins in the ER.The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at thecarboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 andprotein disulfide isomerase. (See catalog #.
Gene ID 821
Application Notes This antibody can be used in Immunocytochemistry, Immunofluorescence,Immunoprecipitation, and Western Blot. By Western blot, this antibody detects an ~90kDa band representing calnexin in human liver, skin, adrenal gland, kidney, heart, colon,testes and ovary extracts. Immunocytochemical staining of calnexin in Huh-7 cells yieldsa pattern consistent with specific endoplasmic reticulum staining.Recommended Starting Dilutions*:ICC: 1/500WB: 1/4000
Restrictions For Research Use only
Format Liquid
Concentration 1.3 mg/ml
Buffer PBS with 0.05% sodium azide. Preservative: 0.05% sodium azide.
Preservative Sodium azide
Storage -20 °C
General Galvin, Krishna, Ponchel et al.: "The major histocompatibility complex class I antigen-binding protein p88 is the product of the calnexin gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8452-6, 1992 (PubMed).

Gething, Sambrook: "Protein folding in the cell." in: Nature, Vol. 355, Issue 6355, pp. 33-45, 1992 (PubMed).

Rajagopalan, Xu, Brenner: "Retention of unassembled components of integral membrane proteins by calnexin." in: Science (New York, N.Y.), Vol. 263, Issue 5145, pp. 387-90, 1994 (PubMed).

Hosts (90), (23), (9)
Reactivities (103), (47), (45), (26), (26), (24), (10), (9), (9), (8), (8), (8), (6), (4), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1)
Applications (113), (49), (39), (29), (25), (25), (21), (10), (10), (8), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (18), (11), (5), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1)
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