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Calnexin (CANX) antibody

Details for Product No. ABIN152677
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Antigen
Synonyms zgc:63524, wu:fe06b12, clgn, clnx, cnx, CANX, CNX, IP90, P90, canx, Cnx, AI988026, D11Ertd153e, 1110069N15Rik, PP90, CG11958, Dmel\CG11958
Reactivity
Human, Mouse (Murine)
(122), (47), (45), (26), (26), (24), (10), (9), (9), (8), (8), (8), (6), (4), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1)
Host
Mouse
(109), (22), (10)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(132), (49), (44), (42), (40), (39), (32), (26), (10), (8), (1), (1)
Pubmed 3 references available
Quantity 0.1 mL
Shipping to United States (Change)
Catalog No. ABIN152677
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Immunogen Focus human hepatoma cell lysate.
Clone AF18
Isotype IgG1
Specificity Calnexin (AF18)
Purification Ascites
Alternative Name Calnexin
Background Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membraneprotein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecularchaperones and participate in the proper folding of polypeptides and their assembly intomultisubunit proteins. Studies indicate that calnexin associates with the majorhistocompatability complex (MHC) class I heavy chains, partial complexes of the T cellreceptor and B cell membrane immunoglobulin, but not with completed receptorcomplexes. It has been shown that calnexin is a chaperone that retains incompletely orimproperly folded proteins in the ER.The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at thecarboxy-terminus of soluble ER resident proteins such as GRP78 and GRP94 andprotein disulfide isomerase. (See catalog #.
Gene Symbol: CANX
Gene ID 821
Application Notes This antibody can be used in Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blot. By Western blot, this antibody detects an ~90 kDa band representing calnexin in human liver, skin, adrenal gland, kidney, heart, colon, testes and ovary extracts. Immunocytochemical staining of calnexin in Huh-7 cells yields a pattern consistent with specific endoplasmic reticulum staining. Recommended Starting Dilutions*: ICC: 1/500 WB: 1/4000 Not tested in other applications. *Optimal dilutions should be determined by the end user.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunoprecipitation 1:10-1:500, Western Blot 1:100-1:2000
Restrictions For Research Use only
Format Liquid
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
General Galvin, Krishna, Ponchel et al.: "The major histocompatibility complex class I antigen-binding protein p88 is the product of the calnexin gene." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8452-6, 1992 (PubMed).

Gething, Sambrook: "Protein folding in the cell." in: Nature, Vol. 355, Issue 6355, pp. 33-45, 1992 (PubMed).

Rajagopalan, Xu, Brenner: "Retention of unassembled components of integral membrane proteins by calnexin." in: Science (New York, N.Y.), Vol. 263, Issue 5145, pp. 387-90, 1994 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN152677
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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