Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (AA 771-789) antibody

Details for Product No. ABIN152684
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Antigen
Synonyms ARNT, zgc:136664, arnt1, hif1b, tango, xArnt, hif1beta, hif-1beta, HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2, D3Ertd557e, Drnt, ESTM42, Hif1b, W08714, mKIAA4051, Arnt1
Epitope
AA 771-789
(13), (9), (5), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Guinea Pig, Human, Monkey, Mouse (Murine), Rat (Rattus)
(85), (42), (37), (21), (13), (12), (9), (9), (1), (1)
Host
Mouse
(52), (33), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), ELISA, Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(73), (28), (24), (21), (12), (10), (9), (5), (4), (1), (1)
Pubmed 6 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN152684
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Immunogen Synthetic peptide, corresponding to amino acids 771-789 of Human HIF1 beta.
Clone 2B10
Isotype IgG1
Specificity Detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT).
Cross-Reactivity (Details) Not yet tested in other species.
Purification IgG purified
Alternative Name HIF1 beta / ARNT
Background HIF1 beta (hypoxia-inducible factor 1 beta, or aryl hydrocarbon receptor nucleartransporter, ARNT) is a transcription factor that contains a basic Helix-Loop-Helix(bHLH) motif as well as two PAS domains. HIF1 beta is required for the activity of thearyl hydrocarbon (Ah) receptor, which is involved in the induction of several enzymesthat are involved in xenobiotic metabolism. Binding of ligands such as dioxin to the Ahreceptor result in the translocation of the Ah receptor from the cytosol to the nucleusthrough its association with HIF1 beta. This complex then initiates transcription of genesinvolved in the activation of PAH procarcinogens. Efficient DNA binding of HIF1 betarequires heterodimerisation with another bHLH protein, such as the Ah receptor.HIF1 beta also plays a critical role in the transcriptional regulation of the adaptiveresponse to hypoxia by forming heterodimers with HIF1 alpha and EPAS1/HIF2 alpha.Hypoxia-induced gene transcription contributes substantially to the malignant phenotypeof solid tumours by promoting characteristic tumour behaviour such as angiogenesis,invasion, metastasis, de-differentiation and enhanced glycolytic metabolism. Alternate Names: anti-HIF1 beta antibody, anti-ARNT antibody, anti-ARNT protein antibody, anti-ArylHydrocarbon Receptor Nuclear Translocator antibody, anti-Dioxin Receptor antibody,anti-Dioxin receptor nuclear translocator antibody, anti-Drnt antibody, anti-HIF 1 betaantibody, anti-HIF 1beta antibody, anti-HIF1B antibody, anti-HIF1beta antibody,anti-Hypoxia Inducible Factor 1 antibody, anti-Hypoxia inducible factor 1 beta antibody,anti-Tango antibody.
Gene Symbol: ARNT
Gene ID 405, 11863
Research Area Transcription Factors
Application Notes This antibody can be used in ELISA, Immunofluorescence, Immunoprecipitation (dilution: assay dependent), Gel Super Shift Assays (dilution: assay dependent) and Western Blot. Chromatin Immunoprecipitation was reported in scientific literature. By Western blot, this antibody detects an 87 kDa protein representing ARNT in COS cell extract. Immunofluorescence staining of ARNT in VT{2} cells (Hepa 1-C4T mutant deficient in ARNT function but stably transfected with the full length human ARNT cDNA) using NB 300-525 yields non-nucleolar, nuclear staining only. No feedback has been obtained on use of this antibody for immunohistochemsitry on tissues. Recommended Starting Dilutions*: IF: 1:1000 WB: 1:4000 *Optimal dilutions should be determined by the end user.
Recommended dilutions: Chromatin Immunoprecipitation, ELISA 1:100-1:2000, Gel Super Shift Assays 1:1-1:100, Immunocytochemistry/Immunofluorescence 1:1000, Immunoprecipitation 1:10-1:500, Western Blot 1:4000
Restrictions For Research Use only
Format Liquid
Buffer PBS with 1.0 mg/ml BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Supplier Images
anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (AA 771-789) antibody Figure 1 shows a Western blot of ARNT on COS cell extract using ABIN152684.
General Hoffman, Reyes, Chu et al.: "Cloning of a factor required for activity of the Ah (dioxin) receptor." in: Science (New York, N.Y.), Vol. 252, Issue 5008, pp. 954-8, 1991 (PubMed).

Hord, Perdew: "Physicochemical and immunocytochemical analysis of the aryl hydrocarbon receptor nuclear translocator: characterization of two monoclonal antibodies to the aryl hydrocarbon receptor nuclear translocator." in: Molecular pharmacology, Vol. 46, Issue 4, pp. 618-26, 1994 (PubMed).

Hirose, Morita, Ema et al.: "cDNA cloning and tissue-specific expression of a novel basic helix-loop-helix/PAS factor (Arnt2) with close sequence similarity to the aryl hydrocarbon receptor nuclear translocator (Arnt)." in: Molecular and cellular biology, Vol. 16, Issue 4, pp. 1706-13, 1996 (PubMed).

Vaziri, Schneider, Sherr et al.: "Expression of the aryl hydrocarbon receptor is regulated by serum and mitogenic growth factors in murine 3T3 fibroblasts." in: The Journal of biological chemistry, Vol. 271, Issue 42, pp. 25921-7, 1996 (PubMed).

Singh, Wyman, Casado et al.: "Treatment of mice with the Ah receptor agonist and human carcinogen dioxin results in altered numbers and function of hematopoietic stem cells." in: Carcinogenesis, Vol. 30, Issue 1, pp. 11-9, 2009 (PubMed).

Amir, Wang, Simons et al.: "SEPT9_v1 up-regulates hypoxia-inducible factor 1 by preventing its RACK1-mediated degradation." in: The Journal of biological chemistry, Vol. 284, Issue 17, pp. 11142-51, 2009 (PubMed).

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