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ARNT antibody (Aryl Hydrocarbon Receptor Nuclear Translocator)

Details for Product anti-ARNT Antibody No. ABIN152684, Supplier: Log in to see
Antigen
  • ARNT
  • arnt1
  • Arnt1
  • bHLHe2
  • D3Ertd557e
  • Drnt
  • ESTM42
  • HIF-1-beta
  • HIF-1beta
  • hif-1beta
  • HIF1-beta
  • Hif1b
  • HIF1B
  • hif1b
  • HIF1BETA
  • hif1beta
  • mKIAA4051
  • tango
  • TANGO
  • W08714
  • xArnt
  • zgc:136664
Reactivity
Amphibian, Human, Mouse (Murine), Primate, Rat (Rattus), Zebrafish (Danio rerio)
157
64
58
29
26
26
18
3
2
2
1
Host
Mouse
75
66
22
Clonality (Clone)
Monoclonal ()
Conjugate
This ARNT antibody is un-conjugated
5
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
135
55
52
43
34
26
16
13
10
7
6
3
3
2
1
1
1
Supplier
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Immunogen Synthetic peptide, corresponding to amino acids 771-789 of Human HIF1 beta.
Clone 2B10
Isotype IgG1
Specificity Detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT).
Cross-Reactivity (Details) Not yet tested in other species.
Purification Protein A or G purified
Alternative Name ARNT/HIF-1 beta (ARNT Antibody Abstract)
Background Gene Symbol: ARNT
Gene ID 405, 11863
UniProt P27540
Application Notes Western Blot 1:4000, Chromatin Immunoprecipitation 1:10-1:500, ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:1000, Immunoprecipitation 1:10-1:500, Gel Super Shift Assays 1:1-1:100
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS with 1.0 mg/mL BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-ARNT antibody (Aryl Hydrocarbon Receptor Nuclear Translocator) (ABIN152684) Figure 1 shows a Western blot of ARNT on COS cell extract using ABIN152684.
Immunofluorescence (IF) image for anti-ARNT antibody (Aryl Hydrocarbon Receptor Nuclear Translocator) (ABIN152684) Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (2B10) [ABIN152684] ...
Immunofluorescence (IF) image for anti-ARNT antibody (Aryl Hydrocarbon Receptor Nuclear Translocator) (ABIN152684) Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (2B10) [ABIN152684] ...
Immunofluorescence (IF) image for anti-ARNT antibody (Aryl Hydrocarbon Receptor Nuclear Translocator) (ABIN152684) Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (2B10) [ABIN152684] ...
Immunofluorescence (IF) image for anti-ARNT antibody (Aryl Hydrocarbon Receptor Nuclear Translocator) (ABIN152684) Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (2B10) [ABIN152684] ...
Product cited in: Mandl, Lieberum, Depping: "A HIF-1α-driven feed-forward loop augments HIF signalling in Hep3B cells by upregulation of ARNT." in: Cell death & disease, Vol. 7, Issue 6, pp. e2284, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Mandl, Lieberum, Dunst, Depping: "The expression level of the transcription factor Aryl hydrocarbon receptor nuclear translocator (ARNT) determines cellular survival after radiation treatment." in: Radiation oncology (London, England), Vol. 10, pp. 229, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Thomas, Harding, Smith, Overdevest, Nitz, Frierson, Tomlins, Kristiansen, Theodorescu: "CD24 is an effector of HIF-1-driven primary tumor growth and metastasis." in: Cancer research, Vol. 72, Issue 21, pp. 5600-12, 2012 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Singh, Wyman, Casado, Garrett, Gasiewicz: "Treatment of mice with the Ah receptor agonist and human carcinogen dioxin results in altered numbers and function of hematopoietic stem cells." in: Carcinogenesis, Vol. 30, Issue 1, pp. 11-9, 2009 (PubMed).

Amir, Wang, Simons, Mabjeesh: "SEPT9_v1 up-regulates hypoxia-inducible factor 1 by preventing its RACK1-mediated degradation." in: The Journal of biological chemistry, Vol. 284, Issue 17, pp. 11142-51, 2009 (PubMed).

Hord, Perdew et al.: "Physicochemical and immunocytochemical analysis of the aryl hydrocarbon receptor nuclear translocator: characterization of two monoclonal antibodies to the aryl hydrocarbon receptor nuclear ..." in: Molecular pharmacology, Vol. 46, Issue 4, pp. 618-26, 1994 (PubMed).

Background publications Hirose, Morita, Ema, Mimura, Hamada, Fujii, Saijo, Gotoh, Sogawa, Fujii-Kuriyama: "cDNA cloning and tissue-specific expression of a novel basic helix-loop-helix/PAS factor (Arnt2) with close sequence similarity to the aryl hydrocarbon receptor nuclear translocator (Arnt)." in: Molecular and cellular biology, Vol. 16, Issue 4, pp. 1706-13, 1996 (PubMed).

Vaziri, Schneider, Sherr, Faller: "Expression of the aryl hydrocarbon receptor is regulated by serum and mitogenic growth factors in murine 3T3 fibroblasts." in: The Journal of biological chemistry, Vol. 271, Issue 42, pp. 25921-7, 1996 (PubMed).

Hoffman, Reyes, Chu, Sander, Conley, Brooks, Hankinson: "Cloning of a factor required for activity of the Ah (dioxin) receptor." in: Science (New York, N.Y.), Vol. 252, Issue 5008, pp. 954-8, 1991 (PubMed).