Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody

Antigen: Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT)

Synonyms: HIF1B, TANGO, bHLHe2, HIF1BETA, HIF-1beta, Drnt, Hif1b, ESTM42, W08714, KIAA4051, mKIAA4051, D3Ertd557e, Arnt1, ARNT, MGC136664, zgc:136664, arnt1, hif1b, tango, xArnt, hif1beta, hif-1beta, AHA-1

Clonality: Monoclonal (2B10)

Host: Mouse

Reactivity: Guinea Pig, Human, Monkey, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: ELISA, Electrophoretic Mobility-Shift Assay (EMSA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN152684

Additional Information

Alternative name: HIF1 beta / ARNT

Gene ID: 405, 11863

Immunogen: Synthetic peptide, corresponding to amino acids 771-789 of Human HIF1 beta.

Cross-Reactivity: Human, Monkey, Mouse (Murine)

Isotype: IgG1

Clone: 2B10

Description: HIF1 beta (hypoxia-inducible factor 1 beta, or aryl hydrocarbon receptor nucleartransporter, ARNT) is a transcription factor that contains a basic Helix-Loop-Helix(bHLH) motif as well as two PAS domains. HIF1 beta is required for the activity of thearyl hydrocarbon (Ah) receptor, which is involved in the induction of several enzymesthat are involved in xenobiotic metabolism. Binding of ligands such as dioxin to the Ahreceptor result in the translocation of the Ah receptor from the cytosol to the nucleusthrough its association with HIF1 beta. This complex then initiates transcription of genesinvolved in the activation of PAH procarcinogens. Efficient DNA binding of HIF1 betarequires heterodimerisation with another bHLH protein, such as the Ah receptor.HIF1 beta also plays a critical role in the transcriptional regulation of the adaptiveresponse to hypoxia by forming heterodimers with HIF1 alpha and EPAS1/HIF2 alpha.Hypoxia-induced gene transcription contributes substantially to the malignant phenotypeof solid tumours by promoting characteristic tumour behaviour such as angiogenesis,invasion, metastasis, de-differentiation and enhanced glycolytic metabolism. Alternate Names: anti-HIF1 beta antibody, anti-ARNT antibody, anti-ARNT protein antibody, anti-ArylHydrocarbon Receptor Nuclear Translocator antibody, anti-Dioxin Receptor antibody,anti-Dioxin receptor nuclear translocator antibody, anti-Drnt antibody, anti-HIF 1 betaantibody, anti-HIF 1beta antibody, anti-HIF1B antibody, anti-HIF1beta antibody,anti-Hypoxia Inducible Factor 1 antibody, anti-Hypoxia inducible factor 1 beta antibody,anti-Tango antibody.

Specificity: Detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT). Species Reactivity: Cross-reacts with Human, Mouse and Non Human Primates. Not yet tested in otherspecies. Localization: Nuclear.

Application Details

Application Notes: Suggested working dilutions: immunofluorescence 1:1,000,Western Blot 1:4,000,Gel Super Shift Assays Assay dependent

Concentration: 1 mg/ml

Purification: Ig Fraction

Buffer: PBS with 1 mg/ml BSA. Preservative: 0.05% Sodium Azide.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Research Area: Transcription Factors

Restrictions: For Research Use only

Images

Figure 1 shows a Western blot of ARNT on COS cell extract using ABIN152684.

Publications

Hoffman, Reyes, Chu et al.: "Cloning of a factor required for activity of the Ah (dioxin) receptor." in: Science (New York, N.Y.), Vol. 252, Issue 5008, pp. 954-8, 1991 (PubMed).

Hord, Perdew: "Physicochemical and immunocytochemical analysis of the aryl hydrocarbon receptor nuclear translocator: characterization of two monoclonal antibodies to the aryl hydrocarbon receptor nuclear translocator." in: Molecular pharmacology, Vol. 46, Issue 4, pp. 618-26, 1994 (PubMed).

Hirose, Morita, Ema et al.: "cDNA cloning and tissue-specific expression of a novel basic helix-loop-helix/PAS factor (Arnt2) with close sequence similarity to the aryl hydrocarbon receptor nuclear translocator (Arnt)." in: Molecular and cellular biology, Vol. 16, Issue 4, pp. 1706-13, 1996 (PubMed).

Vaziri, Schneider, Sherr et al.: "Expression of the aryl hydrocarbon receptor is regulated by serum and mitogenic growth factors in murine 3T3 fibroblasts." in: The Journal of biological chemistry, Vol. 271, Issue 42, pp. 25921-7, 1996 (PubMed).

Singh, Wyman, Casado et al.: "Treatment of mice with the Ah receptor agonist and human carcinogen dioxin results in altered numbers and function of hematopoietic stem cells." in: Carcinogenesis, Vol. 30, Issue 1, pp. 11-9, 2009 (PubMed).

Amir, Wang, Simons et al.: "SEPT9_v1 up-regulates hypoxia-inducible factor 1 by preventing its RACK1-mediated degradation." in: The Journal of biological chemistry, Vol. 284, Issue 17, pp. 11142-51, 2009 (PubMed).