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RYR1 antibody (Ryanodine Receptor 1 (Skeletal))

Details for Product anti-RYR1 Antibody No. ABIN152702, Supplier: Log in to see
Antigen
  • ryr1
  • RYR1
  • alphaRYR
  • CCO
  • MHS
  • MHS1
  • RYDR
  • RYR
  • RYR-1
  • SKRR
  • Ryr1l
  • CRC
  • AI528790
  • Ryr
  • skrr
  • ryanodine receptor 1 (skeletal)
  • ryanodine receptor 1a (skeletal)
  • ryanodine receptor 1, skeletal muscle
  • RYR1
  • ryr1a
  • Ryr1
Alternatives
anti-Amphibian RYR1 antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Amphibian, Cow (Bovine), Dog (Canine), Ferret, Fish, Human, Mouse (Murine), Primate, Rabbit, Rat (Rattus), Sheep (Ovine)
27
6
5
3
2
2
2
2
1
1
1
Host
Mouse
24
3
Clonality (Clone)
Monoclonal ()
Conjugate
This RYR1 antibody is un-conjugated
3
3
3
Application
Blocking Peptide (BP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Neutralization (Neut), Western Blotting (WB)
21
15
10
3
3
2
2
2
2
2
Options
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Available images

Immunogen Partially purified chicken pectoral muscle ryanodine receptor.
Clone 34C
Isotype IgG1
Specificity Detects Ryanodine Receptor (RyR)-1 and RyR-2 isoforms. In chickens, this detects the alpha, beta and cardiac isoforms. This detects RyR-3 in mouse cells. In frog, this detects the alpha and beta isoforms. In fish, this detects the alpha isoform.
Purification Protein A purified
Alternative Name Ryanodine Receptor 1 (RYR1 Antibody Abstract)
Background Gene Symbol: RYR1
Molecular Weight Theoretical MW: 565 kDa
Gene ID 6261
UniProt Q90984
Pathways Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
Application Notes Western Blot 1:5000, Immunohistochemistry 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:1000, Blocking/Neutralizing 50 - 200 Molar excess, Immunofluorescence 1:10 - 1:500May be useful in IF, IHC-Fr, IP, and WB. WB: Detects an approx. 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. IHC: Staining of RyR in chicken brain results in intense staining of cerebral Purkinje neurons. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Ascites with PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-RYR1 antibody (Ryanodine Receptor 1 (Skeletal)) (ABIN152702) Immunohistochemistry-Paraffin: Ryanodine Receptor 1 Antibody (34C) [ABIN152702] - Bio...
Product cited in: Jordan, Jiang, Li, DiMario: "Inhibition of ryanodine receptor 1 in fast skeletal muscle fibers induces a fast-to-slow muscle fiber type transition." in: Journal of cell science, Vol. 117, Issue Pt 25, pp. 6175-83, 2004 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Liu, Zhang, Sharma, Li, Chen, Wagenknecht: "Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor and localization of its amino terminus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 11, pp. 6104-9, 2001 (PubMed).

Chan, Mayne, Holden, Geiger, Mattson: "Presenilin-1 mutations increase levels of ryanodine receptors and calcium release in PC12 cells and cortical neurons." in: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18195-200, 2000 (PubMed).

Yamada, Kanter, Newatia: "Long-chain acylcarnitine induces Ca2+ efflux from the sarcoplasmic reticulum." in: Journal of cardiovascular pharmacology, Vol. 36, Issue 1, pp. 14-21, 2000 (PubMed).

Protasi, Franzini-Armstrong, Allen: "Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle." in: The Journal of cell biology, Vol. 140, Issue 4, pp. 831-42, 1998 (PubMed).

Moore, Nguyen, Galceran, Pessah, Allen: "A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein and function." in: The Journal of cell biology, Vol. 140, Issue 4, pp. 843-51, 1998 (PubMed).

Liu, Pessah: "Molecular interaction between ryanodine receptor and glycoprotein triadin involves redox cycling of functionally important hyperreactive sulfhydryls." in: The Journal of biological chemistry, Vol. 269, Issue 52, pp. 33028-34, 1995 (PubMed). Further details: Blocking Peptide,Neutralization

Airey, Grinsell, Jones, Sutko, Witcher: "Three ryanodine receptor isoforms exist in avian striated muscles." in: Biochemistry, Vol. 32, Issue 22, pp. 5739-45, 1993 (PubMed).

Chen, Airey, MacLennan: "Positioning of major tryptic fragments in the Ca2+ release channel (ryanodine receptor) resulting from partial digestion of rabbit skeletal muscle sarcoplasmic reticulum." in: The Journal of biological chemistry, Vol. 268, Issue 30, pp. 22642-9, 1993 (PubMed).

Walton, Airey, Sutko, Beck, Mignery, Südhof, Deerinck, Ellisman: "Ryanodine and inositol trisphosphate receptors coexist in avian cerebellar Purkinje neurons." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1145-57, 1991 (PubMed).

Airey, Beck, Murakami, Tanksley, Deerinck, Ellisman, Sutko: "Identification and localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle." in: The Journal of biological chemistry, Vol. 265, Issue 24, pp. 14187-94, 1990 (PubMed).

Background publications Olivares, Tanksley, Airey, Beck, Ouyang, Deerinck, Ellisman, Sutko: "Nonmammalian vertebrate skeletal muscles express two triad junctional foot protein isoforms." in: Biophysical journal, Vol. 59, Issue 6, pp. 1153-63, 1991 (PubMed).