Ryanodine Receptor 1 (Skeletal) (RYR1) antibody

Details for Product No. ABIN152702
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Synonyms CRC, RYR, RYR-1, CCO, MHS, MHS1, RYDR, SKRR, AI528790, Ryr, skrr, Ryr1l, ryr1, RYR1, alphaRYR
Amphibian, Cow (Bovine), Dog (Canine), Fish, Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
(18), (7), (6), (3), (2), (2), (2), (1), (1), (1), (1)
(17), (5)
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
(22), (16), (9), (9), (7), (6), (5), (3), (2)
Pubmed 11 references available
Catalog no. ABIN152702
Quantity 0.1 mL
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Immunogen Partially purified chicken pectoral muscle ryanodine receptor.
Clone 34C
Isotype IgG1
Specificity Detects Ryanodine Receptor (RyR)-1 and RyR-2 isoforms.In chickens, this antibodydetects the alpha, beta and cardiac isoforms. This antibody detects RyR-3 in mousecells. In frog, this antibody detects the alpha and beta isoforms. In fish, this antibodydetects the alpha isoform. Species Reactivity: Cross-reacts with Human, Rabbit, Rat, Mouse, Cow, Dog, Sheep, Fish and Amphibians. Not yet tested in other species.
Cross-Reactivity Amphibian, Cow (Bovine), Dog (Canine), Fish, Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
Purification Ascites
Alternative Name Ryanodine receptor 1 / RYR1
Background The Ryanodine Receptor (RyR) is the channel responsible for calcium release frommuscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation innon-muscle cells. The RyR exists as a homotetramer and is predicted to have a shortcytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of theprotein, termed the foot region, is located in the cytoplasm between thetransverse tubule and the SR. Mammalian RyR isoforms are the product of threedifferent genes: RyR-1 is expressed predominantly in skeletal muscle and areas of thebrain, RyR-2 is expressed predominantly in heart muscle but also found in the stomach,endothelial cells and diffuse areas of the brain, and RyR-3 is found in smooth muscleand the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates,the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to themammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.
Application Notes This antibody can be used in Immunofluorescence, Immunohistochemistry-Frozen,Immunoprecipitation, and Western Blot. By Western blot, this antibody detects a 565kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalianvertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms ofthe receptor. Immunohistochemical staining of RyR in chicken brain results in intensestaining of cerebral Purkinje neurons.Recommended Starting Dilutions*:IHC-Fr: 1/1000WB: 1/5000
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05% sodium azide. Preservative: 0.05% sodium azide.
Preservative Sodium azide
Storage -20 °C
General Walton, Airey, Sutko et al.: "Ryanodine and inositol trisphosphate receptors coexist in avian cerebellar Purkinje neurons." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1145-57, 1991 (PubMed).

Olivares, Tanksley, Airey et al.: "Nonmammalian vertebrate skeletal muscles express two triad junctional foot protein isoforms." in: Biophysical journal, Vol. 59, Issue 6, pp. 1153-63, 1991 (PubMed).

Airey, Beck, Murakami et al.: "Identification and localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle." in: The Journal of biological chemistry, Vol. 265, Issue 24, pp. 14187-94, 1990 (PubMed).

Airey, Grinsell, Jones et al.: "Three ryanodine receptor isoforms exist in avian striated muscles." in: Biochemistry, Vol. 32, Issue 22, pp. 5739-45, 1993 (PubMed).

Liu, Pessah: "Molecular interaction between ryanodine receptor and glycoprotein triadin involves redox cycling of functionally important hyperreactive sulfhydryls." in: The Journal of biological chemistry, Vol. 269, Issue 52, pp. 33028-34, 1995 (PubMed).

Chen, Airey, MacLennan: "Positioning of major tryptic fragments in the Ca2+ release channel (ryanodine receptor) resulting from partial digestion of rabbit skeletal muscle sarcoplasmic reticulum." in: The Journal of biological chemistry, Vol. 268, Issue 30, pp. 22642-9, 1993 (PubMed).

Protasi, Franzini-Armstrong, Allen: "Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle." in: The Journal of cell biology, Vol. 140, Issue 4, pp. 831-42, 1998 (PubMed).

Moore, Nguyen, Galceran et al.: "A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein and function." in: The Journal of cell biology, Vol. 140, Issue 4, pp. 843-51, 1998 (PubMed).

Chan, Mayne, Holden et al.: "Presenilin-1 mutations increase levels of ryanodine receptors and calcium release in PC12 cells and cortical neurons." in: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18195-200, 2000 (PubMed).

Yamada, Kanter, Newatia: "Long-chain acylcarnitine induces Ca2+ efflux from the sarcoplasmic reticulum." in: Journal of cardiovascular pharmacology, Vol. 36, Issue 1, pp. 14-21, 2000 (PubMed).

Liu, Zhang, Sharma et al.: "Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor and localization of its amino terminus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 11, pp. 6104-9, 2001 (PubMed).

Hosts (17), (5)
Reactivities (18), (7), (6), (3), (2), (2), (2), (1), (1), (1), (1)
Applications (22), (16), (9), (9), (7), (6), (5), (3), (2)
Epitopes (9), (1), (1)
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