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Ryanodine Receptor 1 (Skeletal) (RYR1) antibody

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Synonyms ryr1, RYR1, alphaRYR, CCO, MHS, MHS1, RYDR, RYR, RYR-1, SKRR, Ryr1l, CRC, AI528790, Ryr, skrr
Amphibian, Cow (Bovine), Dog (Canine), Fish, Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
(26), (5), (3), (1), (1), (1), (1)
(23), (3)
Clonality (Clone)
Monoclonal ()
(3), (3), (3)
Western Blotting (WB), Blocking Reagent (BR), Neutralization (Neut), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(20), (16), (6), (3), (3), (3), (2), (2), (1), (1)
Pubmed 11 references available
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Quantity 0.1 mL
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Immunogen Partially purified chicken pectoral muscle ryanodine receptor.
Clone 34C
Isotype IgG1
Specificity Detects Ryanodine Receptor (RyR)-1 and RyR-2 isoforms. In chickens, this detects the alpha, beta and cardiac isoforms. This detects RyR-3 in mouse cells. In frog, this detects the alpha and beta isoforms. In fish, this detects the alpha isoform.
Cross-Reactivity (Details) Not yet tested in other species.
Purification Ascites
Alternative Name Ryanodine receptor 1 / RYR1 (RYR1 Antibody Abstract)
Background The Ryanodine Receptor (RyR) is the channel responsible for calcium release frommuscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation innon-muscle cells. The RyR exists as a homotetramer and is predicted to have a shortcytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of theprotein, termed the foot region, is located in the cytoplasm between thetransverse tubule and the SR. Mammalian RyR isoforms are the product of threedifferent genes: RyR-1 is expressed predominantly in skeletal muscle and areas of thebrain, RyR-2 is expressed predominantly in heart muscle but also found in the stomach,endothelial cells and diffuse areas of the brain, and RyR-3 is found in smooth muscleand the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates,the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to themammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.
Gene Symbol: RYR1
Gene ID 396112
Application Notes This antibody can be used in Immunofluorescence, Immunohistochemistry-Frozen, Immunoprecipitation, and Western Blot. By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in chicken brain results in intense staining of cerebral Purkinje neurons. Recommended Starting Dilutions*: IHC-Fr: 1/1000 WB: 1/5000 Not tested in other applications. *Optimal dilutions should be determined by the end user.
Recommended dilutions: Blocking/Neutralizing 50-200 molar excess, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:1000, Immunohistochemistry-Paraffin, Immunoprecipitation 1:10-1:500, Western Blot 1:5000
Restrictions For Research Use only
Format Liquid
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.
Product cited in: Yamada, Kanter, Newatia: "Long-chain acylcarnitine induces Ca2+ efflux from the sarcoplasmic reticulum." in: Journal of cardiovascular pharmacology, Vol. 36, Issue 1, pp. 14-21, 2000 (PubMed).

Chan, Mayne, Holden et al.: "Presenilin-1 mutations increase levels of ryanodine receptors and calcium release in PC12 cells and cortical neurons." in: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18195-200, 2000 (PubMed).

Background publications Liu, Zhang, Sharma et al.: "Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor and localization of its amino terminus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 11, pp. 6104-9, 2001 (PubMed).

Moore, Nguyen, Galceran et al.: "A transgenic myogenic cell line lacking ryanodine receptor protein for homologous expression studies: reconstitution of Ry1R protein and function." in: The Journal of cell biology, Vol. 140, Issue 4, pp. 843-51, 1998 (PubMed).

Protasi, Franzini-Armstrong, Allen: "Role of ryanodine receptors in the assembly of calcium release units in skeletal muscle." in: The Journal of cell biology, Vol. 140, Issue 4, pp. 831-42, 1998 (PubMed).

Chen, Airey, MacLennan: "Positioning of major tryptic fragments in the Ca2+ release channel (ryanodine receptor) resulting from partial digestion of rabbit skeletal muscle sarcoplasmic reticulum." in: The Journal of biological chemistry, Vol. 268, Issue 30, pp. 22642-9, 1993 (PubMed).

Liu, Pessah: "Molecular interaction between ryanodine receptor and glycoprotein triadin involves redox cycling of functionally important hyperreactive sulfhydryls." in: The Journal of biological chemistry, Vol. 269, Issue 52, pp. 33028-34, 1995 (PubMed).

Airey, Grinsell, Jones et al.: "Three ryanodine receptor isoforms exist in avian striated muscles." in: Biochemistry, Vol. 32, Issue 22, pp. 5739-45, 1993 (PubMed).

Airey, Beck, Murakami et al.: "Identification and localization of two triad junctional foot protein isoforms in mature avian fast twitch skeletal muscle." in: The Journal of biological chemistry, Vol. 265, Issue 24, pp. 14187-94, 1990 (PubMed).

Olivares, Tanksley, Airey et al.: "Nonmammalian vertebrate skeletal muscles express two triad junctional foot protein isoforms." in: Biophysical journal, Vol. 59, Issue 6, pp. 1153-63, 1991 (PubMed).

Walton, Airey, Sutko et al.: "Ryanodine and inositol trisphosphate receptors coexist in avian cerebellar Purkinje neurons." in: The Journal of cell biology, Vol. 113, Issue 5, pp. 1145-57, 1991 (PubMed).

Catalog No. ABIN152702
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